The Biodiversity and Antimicrobial Activity of Bacteria Isolated from the Bottom Sediments of the Chukchi Sea

Russian Journal of Marine Biology - Two hundred and forty eight strains of heterotrophic microorganisms were isolated from bottom sediments of the Chukchi Sea. A 16S rRNA gene sequence analysis was...     

Effect of anabol on the functional activity indices of mononuclear phagocytes

In this investigation the influence of anabol, a biopolymer preparation incorporated into a complex of extracted components obtained from the surface layer of Lactobacillus bulgaricus cell wall, on some functional activity characteristics of mononuclear phagocytes has been studied. The injection of the preparation in a dose of 20 mg/kg of body weight 24 hours prior to the experiment leads to a decrease in the time necessary for the elimination of one-half of the total amount of ink from the blood stream and to an increase in the content of fibronectin in the blood plasma of rats. The activation of the metabolic activity of resident and induced macrophages, evaluated by the nitro blue tetrazolium reduction test, is observed. On the next day after the injection of anabol the sharp increase of the pool of granulocyte and microphage precursors is observed; the stimulating effect may be retained as long as 3 days. The results thus obtained indicate that anabol acts on different elements of the system of mononuclear phagocytes and can be effective in the complex therapy aimed at the increase of the body resistance to any toxic substances and bacterial infection.     

Effect of radiation, thermal or combined radiation-thermal damage to a population of progenitor cells of the granulocyte-macrophage series in mouse bone marrow

The recovery of a population of granulopoietic-monocytic colony-forming units of mouse bone marrow was only slightly inhibited during the first two weeks after exposure to a mixture of radiation (4 Gy) and heat (burn of 10 per cent of the skin). In the interval between days 14 and 28 following the effect, their number reached the control level, then it decreased again.     

Comparative study of the immunochemical properties of the 5th lactate dehydrogenase isoenzyme from rat liver and skeletal muscle. II. Isolation and characteristics of LDH-5 preparations from rat liver. General analysis of the results

The method of isolation of chromatographically homogenic LDG-5 preparation from the rat liver was elaborated. The preparations obtained in the present study and LDG-5 preparations from skeletal muscles have a common component which is testified by results of the precipitation test in agar. In the rat liver there is an enzymatically non-active antigenic component, which, according to some of its physico-chemical parameters, is very similar with chemically modified forms of LDG-5 dimers.     

Dimethyl sulfoxide as a corrector of disorders of cerebral hemodynamics, oxygen and carbohydrate metabolism of the brain and lipid peroxidation in the early period after intracerebral hemorrhage

Intraperitoneal injection of DMSO (0.7 g/kg) in rats 1h after intracerebral hemorrhage prevented brain conjugated diens and fluorosciene products increase and limited cerebral ++anti-radical activity. In experiments on cats 1h after intracerebral hemorrhage intravenous infusion of DMSO (0.3 g/kg) for 30 min improved cerebral hemodynamic, oxygen and glucose metabolism; cats mortality was decreased.     

Effect of vasopressin on the ATP-ase activity of microsomal fractions of rabbit heart and liver]

It was shown that intravenous injection of vasopressin in a dose of 5 pressor units per 1 kg of body weight led to changes in the ATP-ase activity of the heart and liver microsomes in one hour. These changes coursed in a different direction, i.e. ATP-ase activity of the heart microsomes increased, and ATP-ase activity of the liver microsomes decreased.     

Spin labels in the analysis of Ca-ATPase in the sarcoplasmic reticulum of rabbit skeletal muscles in hypercholesteremia

The method of electron paramagnetic resonance with spin-labeled maleimide was used to study variation of the structure of Ca-ATPase of the sarcoplasmic reticulum (SR) in rabbit skeletal muscles under long-term hypercholesterolemia (HC). The rate of the maleimide spin label binding with Ca-ATPase of the SR was decreased in HC, which correlated with a lesser access of spin-labeled thiol groups for potassium ferricyanide and sodium ascorbate. HC led to a considerable reduction in the lability and to enhancement of hydrophobia of the spin-labeled fragment of the enzyme. It is concluded that the disordered function of the SR Ca-pump is a consequence of structural changes in the Ca-ATPase molecule in HC.