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101.
Kim DW Chung HK Park KC Hwang JH Jo YS Chung J Kalvakolanu DV Resta N Shong M 《Molecular endocrinology (Baltimore, Md.)》2007,21(12):3039-3049
102.
Tak H Jang E Kim SB Park J Suk J Yoon YS Ahn JK Lee JH Joe CO 《Cellular signalling》2007,19(11):2379-2387
The signal pathway by which 14-3-3epsilon inhibits cell migration induced by MAPK-activated protein kinase 5 (MK5) was investigated in cultured HeLa cells. Both in vivo and in vitro analyses have revealed that 14-3-3epsilon interacts with MK5. 14-3-3epsilon bound to MK5 inhibits the phosphorylation of HSP27, a known substrate of MK5. Disturbance of actin cytoskeleton organization by 14-3-3epsilon was shown in transfected cells transiently expressing 14-3-3epsilon as well as established cells stably expressing 14-3-3epsilon. Moreover, overexpression of 14-3-3epsilon resulted in the inhibition of cell migration induced by MK5 overexpression or TNFalpha treatment. Our results suggest that 14-3-3epsilon bound to MK5 inhibits cell migration by inhibiting the phosphorylation of HSP27 whose phosphorylation regulates F-actin polymerization, actin cytoskeleton organization and subsequent actinfilament dynamics. 相似文献
103.
Song ES Park SA Kim SH Cho YJ Ahn BY Ahn BC Lee NG 《FEMS immunology and medical microbiology》2007,51(3):496-504
CIA07 is an immunostimulatory agent composed of bacterial DNA fragments and modified lipopolysaccharide, which has antitumor activity against bladder cancer in mice. In this study, the adjuvant activity of CIA07 was evaluated using hepatitis B virus surface antigen (HBsAg) as the immunogen. Mice were immunized intramuscularly three times at 1-week intervals with HBsAg alone or in combination with alum, bacterial DNA fragments, modified lipopolysaccharide, CIA07 or CpG1826, and immune responses were assessed. At 1 week after the final injection, the HBsAg-specific total serum IgG antibody titer in CIA07-treated mice was 14 times higher than that in animals administered antigen alone, six times higher than in mice given alum or bacterial DNA fragments and twice as high as those treated with modified lipopolysaccharide or CpG1826, and remained maximal until 8 weeks postimmunization. Animals receiving antigen alone or plus alum displayed barely detectable HBsAg-specific serum IgG2a antibody responses. However, coadministration of CIA07 with antigen led to markedly enhanced serum IgG2a antibody titer and IFN-gamma(+) production in splenocytes, indicating that CIA07 effectively induces Th1-type immune responses. In addition, the number of HBsAg-specific CD8(+) T cells in peripheral blood mononuclear cells was elevated in CIA07-treated mice. These data clearly demonstrate that CIA07 is able to induce both cellular and humoral immune responses to HBsAg, and confirm its potential as an adjuvant in therapeutic vaccines for hepatitis B virus infections. 相似文献
104.
Ki-Yeol Kim Dong Hyuk Ki Ha Jin Jeong Hei-Cheul Jeung Hyun Cheol Chung Sun Young Rha 《BMC bioinformatics》2007,8(1):218
Background
With microarray technology, variability in experimental environments such as RNA sources, microarray production, or the use of different platforms, can cause bias. Such systematic differences present a substantial obstacle to the analysis of microarray data, resulting in inconsistent and unreliable information. Therefore, one of the most pressing challenges in the field of microarray technology is how to integrate results from different microarray experiments or combine data sets prior to the specific analysis. 相似文献105.
Baumler DJ Hung KF Jeong KC Kaspar CW 《Extremophiles : life under extreme conditions》2007,11(6):841-851
Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and
survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined
that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer
studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production
in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained
enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine
to produce H2S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase.
Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing
acidophile, thus identifying a new contributor to the global sulfur cycle. 相似文献
106.
Djordjevic MA Oakes M Li DX Hwang CH Hocart CH Gresshoff PM 《Journal of proteome research》2007,6(9):3771-3779
Molecular signaling interactions in the plant apoplast are important for defense and developmental responses. We examined the soybean proteome of the apoplastic conduit of root-to-shoot communication, the xylem stream, using gel electrophoresis combined with two types of tandem mass spectrometry. We examined soybeans for the presence of a Bradyrhizobium japonicum-induced, long distance developmental signal that controls autoregulation of nodulation (AON) to determine if xylem proteins (XPs) were involved directly or indirectly in AON. The xylem and apoplast fluids collected in hypocotyl, epicotyl, and stem tissue contained a highly similar set of secreted proteins. The XPs were different from those secreted from imbibing seed implying they play important basic roles in xylem function. The XPs of wild-type and nts1007 plants were indistinguishable irrespective of plant age, inoculation status, or time after inoculation suggesting that none was directly involved in AON. XPs were continuously loaded into the xylem stream, as they were present even 28 h after shoot decapitation. These results were consistent with semiquantitative RT-PCR studies that examined the expression of genes corresponding to the XPs under inoculated or uninoculated conditions. Monitoring the expression of XP genes by RT-PCR showed that four possessed root biased expression. This suggested that the corresponding protein products could be produced in roots and travel long distances to shoots. Of these, a species of lipid transfer protein is a candidate for a water-soluble, long-distance signal-carrier due to the presence of hydrophobic clefts that bind known plant signals in vitro. Two soybean XPs identified in this study, lipid transfer protein and Kunitz trypsin inhibitor (KTI), have known roles in plant signaling. 相似文献
107.
108.
Live cell imaging compatible immobilization of Chlamydomonas reinhardtii in microfluidic platform for biodiesel research 下载免费PDF全文
109.
110.
Enhancing functional expression of heterologous proteins through random substitution of genetic codes in the 5' coding region 下载免费PDF全文