珍稀濒危物种堇叶紫金牛高效快繁体系的建立

筛选堇叶紫金牛(Ardisia violacea)野生优株,以其当年新发带休眠腋芽茎段为外植体,通过启动培养、丛生芽诱导增殖、壮苗培养、生根培养和炼苗移栽等过程建立其组培快繁技术体系。研究结果表明,最佳启动培养基为MS+0.80 mg·L~(–1)KT+0.10 mg·L~(–1) NAA+0.10 mg·L~(–1) IBA,腋芽萌发率达92.60%;最佳丛生芽诱导增殖培养基为MS1+0.50 mg·L~(–1) TDZ+0.10mg·L~(–1) NAA,平均增殖系数达8.60;最佳壮苗培养基为MS+1.00 mg·L~(–1) KT+0.50 mg·L~(–1) NAA;最佳生根培养基为1/2MS+2.00 mg·L~(–1) IBA+1.00 mg·L~(–1) NAA+1.00 mg·L~(–1) AC,平均生根率达98.70%;采用松鳞和泥炭(2:1,v/v)作为炼苗基质,炼苗成活率可达85.30%。实验成功建立了堇叶紫金牛高效组培快繁技术体系,经验证该体系能够满足规模化生产的需求。     

Ruthenium ammine complexes as electron acceptors for growth stimulation by plasma membrane electron transport

Ammineruthenium(III) complexes have been found to act as electron acceptors for the transplasmalemma electron transport system of animal cells. The active complexes hexaammineruthenium(III), pyridine pentaammineruthenium(III), and chloropentaammineruthenium(III) range in redox potential (E ₀) from 305 to –42 mV. These compounds also act as electron acceptors for the NADH dehydrogenase of isolated plasma membranes. Stimulation of HeLa cell growth, in the absence of calf serum, by these compounds provides evidence that growth stimulation by the transplasma membrane electron transport system is not entirely based on reduction and uptake of iron.     

黄芪有效成分对氧自由基清除作用的ESR研究

用电子自旋共振技术研究了黄芪总黄酮(TFA)、黄芪总皂甙(TSA)和黄芪总多糖(TPA)对次黄嘌呤／黄嘌呤氧化酶体系产生的超氧阴离子自由基和H₂O₂－Fe²⁺体系产生的羟自由基的清除作用．结果表明,这3种成分均有清除氧自由基的作用;对超氧阴离子自由基的清除效能大于对羟自由基的清除作用;其作用强度依次为TFA＞TSA＞TPA．结果提示清除氧自由基可能是黄芪抗衰老的主要机理之一,TFA和TSA是黄芪抗氧化作用的主要药理活性成分．     

日本落叶松人工林种群自然稀疏规律的研究

一、前言日本落叶松(Larix kaempferi)原产日本。种群地理分布范围为北纬35°20′—38°10′,东经136°45′—140°30′,海拔高度为1000—2800m。1900年引入我国,以其较强的适应性“定居”下来,而且生长速度在抚顺地区远比长白落叶松(L.olgensis var.changpaiensis)、兴安落叶松(L.gmelinii)为快;我国黑龙江、吉林、辽宁、山东、山西、陕西、北京、河南、河北、江西、湖北、四川以及新疆等地都有人工栽培,而以吉林、辽宁东部栽培面积较大,在年平均气温2.5—12.0℃、年降水量500—1400mm 的水热条件下能正常生长。     

松嫩平原盐碱化草地治理方法的比较研究

采用生物、化学和物理方法对盐碱化草地治理进行了比较研究．生物方法采用枯草处理;化学方法采用石膏处理;物理方法采用铺沙处理,种植羊草、野大麦和碱茅３种牧草．结果表明,３种方法均能降低土壤ｐＨ值、电导率和增加土壤含水率．在生物治理中,羊草和野大麦在枯草量达１５００ｇ·ｍ－２,碱茅在枯草量达１０００ｇ·ｍ－２时,便可良好生长．在化学治理中,石膏施用量达１．２５ｋｇ·ｍ－２,即可达到改良效果．在物理方法治理中,羊草和野大麦在沙层厚度为１３ｃｍ,碱茅在１１ｃｍ时即可正常生长发育．     

污染土壤中多环芳烃生物降解的调控研究

选用温度、湿度、表面活性剂ＴＷ８０和ＣＮＰ比４个因素为调控因子,采用正交法进行周期为１５０天的实验研究．结果表明,３０天后,土壤中ＰＡＨｓ的降解率可达４４．５～７４．６％,６０天后,达７０．４～９３．７％,降解率的不同与调控条件显著相关．在此期间,降解最佳条件为４０℃,湿度２５％,ＣＮＰ比为１２０１０１,ＴＷ８０分别为２００～５００ｍｇ·ｋｇ－１．实验结束时,土壤中ＰＡＨｓ的降解率达９１．２～９９．８％．降解的最佳条件是４０℃,湿度１５％．经Ｒ值判别表明,不同时期各因子对ＰＡＨｓ降解影响有所不同．温度对ＰＡＨｓ降解影响较大,表面活性剂对土壤中ＰＡＨｓ的生物降解有调控作用．     

新型戊型肝炎诊断试剂盒的研制及其应用

用ＨＥＶＯＲＦ３合成肽及ＯＲＦ２重组抗原研制成新型ＨＥＶＥＩＡ诊断试剂盒。与ＧｅｎｌａｂｓＨＥＶＥＩＡ检测比较,灵敏度和特异性均达１００％（６０／６０）。三批试剂精密性测定均＜１０％。该试剂盒置４℃８个月或３７℃４ｄ保持稳定。检测不同肝炎患者ＨＥＶ抗体,发现急性非甲非乙非丙肝炎中有６３．２％,甲肝有１３．４％,乙肝有８．３％,丙肝有６．６％,正常人群为２．９％。所研制的戊型肝炎诊断试剂盒,灵敏度高,特异性强,精密性好,稳定性合格。适用于戊型肝炎诊断及戊肝病毒感染的流行病学调查     

林可链霉菌黑色素生物合成基因的克隆与表达

以pIJ702的melCl－C2基因为探针杂交林可链霉菌（Streptomyceslincolnensis）78－11染色体DNA,呈现出3．2kb的BamHI片段和2．6kb的SphI片段等一系列阳性条带。构建了含3．0～3．5kbBamHI片段的林可链霉菌78-11基因文库,从中分离克隆了黑色素生物合成基因melCl和melC2,并测定了含有mel基因的重组子pRSB336插入片段的全部DNA顺序。3152bpBamHI片段含有5个开放阅读框架,其中melCl和melC2与链霉菌属三个种的相应基因具有较高的同源性。此外,林可链霉菌78－11的melC2基因产物与人和鼠的酪氨酸酶轻微同源,分别为17．3％和24．5％。种种迹象表明,melCl、melC2和orf3组成黑色素生物合成操纵子结构。为了进一步鉴定上述克隆的林可链霉菌78-11黑色素生物合成基因,构建了分别含有新霉素抗性基因启动子和正反方向mel基因的重组质粒pPZ518和pPZ519,并转化变铅青链霉菌TK23。随机挑选的12个pPZ518转化子在R2YE培养基上均能分泌淡褐色色素,而所有的pPZ519和pES1转化子则都呈白色。     

The structure analysis and antigenicity study of the N protein of SARS-CoV

The Coronaviridae family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with o     

Differential effects of two growth inhibitory K vitamin analogs on cell cycle regulating proteins in human hepatoma cells

A comparison was made between two K vitamin analogs. Growth in vitro of Hep G2 hepatoma cells was inhibited both by Compound 5 (Cpd 5), a recently synthesized thioalkyl analog of vitamin K or 2-(2-mercaptoethanol)-3-methyl-1, 4-naphthoquinone, as well as by synthetic vitamin K3 (menadione). Using synchronized Hep G2 hepatoma cells, the actions of both Cpd 5 and vitamin K3 on cell cycle regulating proteins were examined. Cpd 5 decreased the levels of cyclin D1, Cdk4, p16, p21 and cyclin B1. By contrast, VK3 only decreased the level of cyclin D1, but had no effect on the levels of Cdk4, p16 or p21. Interestingly, both VK3 and VK2 increased the levels of p21. The naturally occurring K vitamins had little effect on cell growth and none on the cyclins or Cdks. Amounts and activity of the G1/S phase controlling Cdc25A were measured. We found that Cpd 5 directly inhibited both Cdc25A activity and its protein expression, whereas VK3 did not. Thus, the main effects of Cpd 5 were on G1 and S phase proteins, especially Cdk4 and Cdc25A amounts in contrast to VK3. Computer docking studies of Cpd 5 and VK3 to Cdc25A phosphatase showed three binding sites. In the best conformation, Cpd 5 was found to be closer to the enzyme active site than VK3. These findings show that Cpd 5 represents a new class of anticancer agent, being a protein tyrosine phosphatase (PTP) antagonist, that binds to Cdc25A with suppression of its activity. Tumors expressing high levels of oncogenic Cdc25A phosphatase may thus be susceptible to the growth inhibitory activities of this class of compound.