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971.
Ji Guo Su Xiao Ming Han Xiao Zhang Yan Xue Hou Jian Zhuo Zhu Yi Dong Wu 《Journal of biomolecular structure & dynamics》2016,34(3):560-571
Protein collective motions play a critical role in many biochemical processes. How to predict the functional motions and the related key residue interactions in proteins is important for our understanding in the mechanism of the biochemical processes. Normal mode analysis (NMA) of the elastic network model (ENM) is one of the effective approaches to investigate the structure-encoded motions in proteins. However, the motion modes revealed by the conventional NMA approach do not necessarily correspond to a specific function of protein. In the present work, a new analysis method was proposed to identify the motion modes responsible for a specific function of proteins and then predict the key residue interactions involved in the functional motions by using a perturbation approach. In our method, an internal coordinate that accounts for the specific function was introduced, and the Cartesian coordinate space was transformed into the internal/Cartesian space by using linear approximation, where the introduced internal coordinate serves as one of the axes of the coordinate space. NMA of ENM in this internal/Cartesian space was performed and the function-relevant motion modes were identified according to their contributions to the specific function of proteins. Then the key residue interactions important for the functional motions of the protein were predicted as the interactions whose perturbation largely influences the fluctuation along the internal coordinate. Using our proposed methods, the maltose transporter (MalFGK2) from E. Coli was studied. The functional motions and the key residue interactions that are related to the channel-gating function of this protein were successfully identified. 相似文献
972.
973.
974.
大鼠眼球和泪腺中睫状神经营养因子及其受体的组织定位 总被引:1,自引:0,他引:1
目的观察睫状神经营养因子(CNTF)及其受体CNTFRα在大鼠眼球、泪腺上的分布情况.方法取雄性SD大鼠两侧眼球和泪腺,作石蜡切片,用免疫组织化学ABC法染色检测眼球、泪腺中CNTF和CNTFRα的免疫阳性反应.结果 CNTF和CNTFRα的免疫阳性反应产物在眼球和泪腺的组织定位基本相同,包括角膜上皮细胞、固有层神经纤维、角膜细胞、内皮细胞,虹膜上皮细胞,睫状体上皮细胞,晶状体上皮,视网膜色素上皮细胞、苗勒细胞、节细胞层细胞,球结膜上皮细胞,泪腺腺细胞及导管上皮.结论 CNTF及其受体选择性地分布于眼球和泪腺的一些细胞中,且多数细胞与房水和泪液的产生和接触有关. 相似文献
975.
松山自然保护区各功能区植被动态及变化格局 总被引:1,自引:0,他引:1
作为全球生态保育的基石,保护区管理和设计成为研究热点。本研究旨在描述不同功能区在相应保护措施下植被的差异性变化。运用遥感和GIS技术以及景观分析手段,利用两期Landsat5TM影像提取出北京松山国家级自然保护区1987、2001年的归一化差值植被指数(NormalizedDifferenceVegetationIndex,NDVI)信息,对植被动态进行分析,并按变化率分为减少、无变化和增加3种类型,利用景观指数刻画出变化格局,就植被动态及其变化格局在不同功能区的空间分异性进行比较。得到以下主要结论:(1)保护区建立后植被大体稳定并有所改善,形成核心区>缓冲区>实验区的植被覆盖梯度。(2)在绝对保护措施下,核心区植被稳定性高并得到进一步改善。植被稳定类型集中于区域中心,增加类型聚集在区域边缘,减少类型与自然植被动态特征相符呈随机分布状态。(3)受经营、开发措施影响,实验区稳定性低,变化面积大、变幅大、增加与衰退共存,植被覆盖居全保护区最低且有退化趋势。(4)缓冲区植被动态呈现出一定的随机分布格局。 相似文献
976.
椿叶花椒叶挥发油化学成分的研究 总被引:2,自引:0,他引:2
采用气相色谱-质谱联用仪分析,研究了用水蒸气蒸馏法提取的椿叶花椒叶挥发油的化学成分。结果表明:鉴定出33种成分,占挥发油总离子流色谱峰面积的99.99%。椿叶花椒叶挥发油的主要成分为2-壬酮,芳樟醇,β-水芹烯。 相似文献
977.
采用酶切连接和重叠PCR连接两种方法将抗黑色素瘤单链抗体基因和去除N端信号肽的金黄色葡萄球菌肠毒素A基因进行融合,并将融合基因克隆于pET28a表达载体上,转化大肠杆菌BL21(DE3)。用NiNTA系统对表达产物进行分离、纯化。MTT法检测融合蛋白对黑色素瘤细胞的体外抑制率。结果表明6HisScFvSEA融合蛋白可在E.coli BL21(DE3)中稳定表达,表达量占菌体蛋白的30%,主要以包涵体的形式存在。融合蛋白可通过激活效应细胞对表达相关抗原的黑色素瘤细胞发挥抑制作用。 相似文献
978.
Wu Xinghan Liu Chuanliang Li Zihaoran Gai Chengcheng Ding Dejun Chen Weijuan Hao Fengyun Li Wentong 《Molecular and cellular biochemistry》2020,473(1-2):217-228
Molecular and Cellular Biochemistry - Ferroptosis is a newly discovered form of regulated cell death and characterized by an iron-dependent accumulation of lethal lipid reactive oxygen species... 相似文献
979.
Journal of Plant Growth Regulation - With the increasing popularity of urban landscaping, there is a greater need to address iron deficiency and chlorosis in Cinnamomum camphora. Beneficial... 相似文献
980.
Jiqiang Zhang Ziru Niu Tianhua Li Yanqiang Wu Rui Xi Yuhua Li Guanghua Li Jialin Hou 《Phyton》2020,89(1):147-166
Considering the current low level of mechanization for domestic green
onion planting and the high labor intensity of artificial planting, a 2ZYX-2 green
onion ditching and transplanting machine, which can complete ditching, ridging,
transplanting, repression, soil covering and other operations, is designed in this
study. The Central Composite test design method was carried out with the speed
of the transplanting machine, the depth of the opener and the horizontal position
of the opener as the experimental factors and with the qualification ratio of
perpendicularity, the variation coefficient of the plant spacing and the
qualification ratio of the planting depth as the test index. Through the analysis of
the model interaction and response surface, the change laws that the influence
the machine’s forward speed, the depth of the opener and the horizontal position
of the opener were studied. The regression model was optimized by
Design-Expert 8.0.6 software, and the accuracy of the predicted results was
verified by experiments. The optimal working parameters showed that the
forward speed of the machine was 0.06 m/s, the depth of the opener was 102 mm,
and the horizontal position of the opener was 29 mm. Under conditions of
optimal working parameters, the qualification rate of the verticality was 86.83%,
the coefficient of variation for the plant spacing was 2.77, and the pass rate of
planting depth was 88.26%. The research related to the thesis can provide a
reference for the mechanized planting of green onion, which is of great
significance to the cost-effectiveness of the green onion industry. 相似文献