全文获取类型
收费全文 | 2123篇 |
免费 | 173篇 |
国内免费 | 194篇 |
专业分类
2490篇 |
出版年
2024年 | 4篇 |
2023年 | 30篇 |
2022年 | 68篇 |
2021年 | 105篇 |
2020年 | 68篇 |
2019年 | 109篇 |
2018年 | 102篇 |
2017年 | 72篇 |
2016年 | 90篇 |
2015年 | 140篇 |
2014年 | 145篇 |
2013年 | 134篇 |
2012年 | 202篇 |
2011年 | 166篇 |
2010年 | 98篇 |
2009年 | 80篇 |
2008年 | 107篇 |
2007年 | 86篇 |
2006年 | 75篇 |
2005年 | 72篇 |
2004年 | 39篇 |
2003年 | 48篇 |
2002年 | 54篇 |
2001年 | 35篇 |
2000年 | 32篇 |
1999年 | 48篇 |
1998年 | 19篇 |
1997年 | 31篇 |
1996年 | 20篇 |
1995年 | 29篇 |
1994年 | 30篇 |
1993年 | 16篇 |
1992年 | 35篇 |
1991年 | 22篇 |
1990年 | 19篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 7篇 |
1984年 | 11篇 |
1983年 | 6篇 |
1982年 | 1篇 |
1981年 | 2篇 |
排序方式: 共有2490条查询结果,搜索用时 15 毫秒
991.
992.
993.
994.
Xiaonan Zhuang Jun Ma Sisi Xu Zhongcui Sun Rong Zhang Meng Zhang Gezhi Xu 《Journal of cellular and molecular medicine》2021,25(1):147-160
We investigated how Src-homology 2-domain phosphatase-1 (SHP-1) regulates the inflammatory response in endotoxin-induced uveitis (EIU), and the signalling pathways involved. One week after intravitreal injection of short hairpin RNA targeting SHP-1 or SHP-1 overexpression lentivirus in rats, we induced ocular inflammation with an intravitreal injection of lipopolysaccharide (LPS). We then assessed the extent of inflammation and performed full-field electroretinography. The concentrations and retinal expression of various inflammatory mediators were examined with enzyme-linked immunosorbent assays and Western blotting, respectively. SHP-1 overexpression and knockdown were induced in Müller cells to study the role of SHP-1 in the LPS-induced inflammatory response in vitro. Retinal SHP-1 expression was up-regulated by LPS. SHP-1 knockdown exacerbated LPS-induced retinal dysfunction and increased the levels of proinflammatory mediators in the retina, which was abrogated by a c-Jun N-terminal kinase (JNK) inhibitor (SP600125). SHP-1 overexpression had the opposite effects. In Müller cells, the LPS-induced inflammatory response was enhanced by SHP-1 knockdown and suppressed by SHP-1 overexpression. SHP-1 negatively regulated the activation of the transforming growth factor-β-activated kinase-1 (TAK1)/JNK pathway, but not the nuclear factor-κB pathway. These results indicate that SHP-1 represses EIU, at least in part, by inhibiting the TAK1/JNK pathway and suggest that SHP-1 is a potential therapeutic target for uveitis. 相似文献
995.
Zhengfei Shan Liqin Wei Shengqiang Yu Shanling Jiang Yue Ma Chengjun Zhang Jiantao Wang Zhenli Gao Fengchun Wan Guimin Zhuang Jitao Wu Dongfu Liu 《Journal of cellular physiology》2019,234(3):2778-2787
This study was aimed at exploring the underlying mechanisms of ketamine in the SV-40 immortalized human ureteral epithelial (SV-HUC-1) cells. The viability and apoptosis of SV-HUC-1 cells treated with 0.01, 0.1, and 1 mM ketamine were respectively detected via cell counting kit-8 (CCK-8) assay and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining. Reactive oxygen species (ROS) level was measured through ROS probe staining. Apoptosis-related proteins (B-cell lymphoma 2 [Bcl-2] and Bax) and autophagy-associated proteins (light chain 3-I [LC3-I] and LC3-II) were determined by western blot or immunofluorescent assay. Additionally, transmission electron microscopy (TEM) was used to evaluate the formation of autophagosomes. After cotreatment of 3-methyladenine (3-MA) or N-acetyl-l -cysteine (NAC), the biological functions of SV-HUC-1 cells were analyzed to determine the association of ROS with cell viability and autophagy. CCK-8 assay and TUNEL staining indicated that ketamine effectively decreased the viability of SV-HUC-1 cells and accelerated apoptosis of SV-HUC-1 cells through regulating the expression level of IKBα (phospho), nuclear factor кB (P65), Bcl-2, and Bax proteins. Enhanced ROS production was also confirmed in ketamine-treated SV-HUC-1 cells treated with ketamine. Ketamine-induced autophagosomes in SV-HUC-1 cells were observed by means of TEM, and increased levels of LC3 II/I ratio and Beclin 1 were examined through western blot and immunofluorescent assay. Furthermore, ketamine exerted effects on SV-HUC-1 cells in a dose-dependent and time-dependent manner. Additionally, cotreatment of NAC with 3-MA significantly attenuated the ROS level and suppressed the cell autophagy. Ketamine promoted SV-HUC-1 cell autophagy and impaired the cell viability of SV-HUC-1 cells by inducing ROS. 相似文献
996.
Ying Ye Yanan Song Juhua Zhuang Guoyu Wang Jing Ni Wei Xia 《Journal of cellular physiology》2019,234(2):1880-1888
Echinacoside (ECH) is a phenylethanoid glycoside extracted from a Chinese herbal medicine, Cistanches salsa. ECH possesses many biological properties, including anti-inflammation, neural protection, liver protection, and antitumor. In the current study, we aimed to explore the effects of ECH on hepatocellular carcinoma (HCC) and the underlying mechanisms. The results showed that ECH could attenuate diethylnitrosamine (DEN)-induced HCC in mice, and exerted antiproliferative and proapoptotic functions on HepG2 HCC cell line. ECH exposure in HepG2 cells dose-dependently reduced the phosphorylation of AKT (p-AKT) and enhanced the expression of p21 (a cell cycle inhibitor) and Bax (a proapoptotic protein). Furthermore, ECH significantly suppressed insulin-like growth factor-1-induced p-AKT and cell proliferation. These data indicated that phosphoinositide 3-kinase (PI3K)/AKT signaling was involved in the anti-HCC activity of ECH. Gene set enrichment analysis results revealed a positive correlation between the PI3K pathway and triggering receptors expressed on myeloid cells 2 (TREM2) expression in HCC tissues. ECH exposure significantly decreased TREM2 protein levels in HepG2 cells and DEN-induced HCC. Furthermore, ECH-mediated proliferation inhibition and AKT signaling inactivation were notably attenuated by TREM2 overexpression. In conclusion, ECH exerted its antitumor activity via decreasing TREM2 expression and PI3K/AKT signaling. 相似文献
997.
998.
999.
Long Yuming Zhuang Kai Ji Zhonghai Han Yaru Fei Yanqing Zheng Wen Song Zhi Yang Heng 《Neurochemical research》2019,44(4):994-1004
Neurochemical Research - Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-d-glucose (2-DG) has been... 相似文献
1000.