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101.
Adherence Patterns and DNA Probe Types of Escherichia coli Isolated from Diarrheal Patients in China
Jian-Guo Xu Bo-Qun Cheng Yan-Ping Wu Li-Bao Huang Xin-He Lai Bing-Yang Liu Xing-Zu Lo Hun-Fen Li 《Microbiology and immunology》1996,40(2):89-97
One hundred and seventy-two strains of Escherichia coli isolated from diarrheal patients in Beijing, P. R. China, were analyzed for plasmid DNA profile, HEp-2 cell adherence ability and reactivity to 10 previously described DNA probes. They had not been recognized as pathogenic E. coli in China. Of the 110 strains tested, 76 (69%) contained one or multiple large plasmids. Of the 71 strains with the large plasmids 64 could adhere to HEp-2 cells. Of the 172 strains, 102 (59.3%) were hybridized with at least one of the 10 probes. Of those, seven strains hybridized with enteroaggregative E. coli (EAggEC) probe. Their serotypes were O128 (two strains), O6 (one strain), and O111 (one strain). Three strains were untypable. Six and three strains were hybridized with enteropathogenic E. coli (EPEC) attaching and effacing genes (eae) or EPEC adherence factor (EAF) probe, respectively. Two non-O157: H7 strains hybridized with enterohemorrhagic E. coli (EHEC) probe. Seventy-two strains (41.9%) hybridized with shiga-like toxin 2 or 1 (SLT2 or SLT1) probes. Among the SLT1 or SLT2 probe-positive strains, 54 hybridized with invasive (INV) plasmid probe developed for identification of enteroinvasive E. coli (EIEC) and Shigella species. The INV and SLT probe-positive strains might represent a new variety of verotoxin-producing E. coli (VTEC). 相似文献
102.
A simple and reliable method is described which combines ultrafiltration technique with atomic absorption spectrophotometry
to determine the Zn fractions in human blood plasma and seminal plasma. Ultrafiltrable, loosely bound, and firmly bound Zn
can be measured using this method in the presence or absence of ethylene-diaminetetraacetic acid (EDTA). The YMT membranes
for the ultrafiltration must be rinsed thoroughly before use. In contrast to Zn in blood plasma, a large part of Zn in the
seminal plasma was found to be ultrafiltrabe. This method can be applied to study the physiologically active part of Zn in
body fluids related to various disease states. 相似文献
103.
104.
Estimation of the position and effect of a lethal factor locus on a molecular marker linkage map 总被引:3,自引:0,他引:3
R. Cheng A. Saito Y. Takano Y. Ukai 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(4):494-502
In the mapping of DNA markers the distortion of segregation of marker genotypes is often observed, which may be caused by a lethal factor acting in filial generations derived from distant crosses. A method is presented for estimating the recombination values between a lethal factor locus and neighboring molecular markers, and the relative viability or fertilization ability of gametes or zygotes affected by the lethal factor in an F2 population using the maximum likelihood method and the expectation conditional maximization (ECM) algorithm. Three selection models of gamete or zygote were considered, and the most likely one was determined by goodness of fit of the observed frequency of the phenotypes to the expected ones under the models. The method was applied to segregation data of molecular markers of an F2 population consisting of 144 individuals derived from a cross between an Indica and a Japonica rice variety. The presence of a lethal factor locus (L) located on chromosome III that caused partial gametic selection in both the male and female sides was suggested. The locus L was tightly linked to RFLP marker number 23 of the RFLP linkage map of Saito et al. (1991a), and the fertilization chance of a male or female gamete possessing the lethal factor was, on average, 41.5% of that of the normal gamete. 相似文献
105.
Packing defects in lipid bilayer play a significant role in the biological activities of cell membranes. Time-resolved fluorescence depolarization has been used to detect and characterize the onset of packing defects in binary mixtures of dilinoleoylphosphatidylethanolamine/1-palmitoyl-2- oleoylphosphatidylcholine (PE/PC). These PE/PC mixtures exhibit mesoscopic packing defect state (D), as well as one-dimensional lambellar liquid crystalline (L alpha) and two-dimensional inverted hexagonal (HII) ordered phases. Based on previous electron microscopic investigations, this D state is characterized by the presence of interlamellar attachments and precursors of HII phase between the lipid layers. Using a rotational diffusion model for rod-shaped fluorophore in a curved matrix, rotational dynamics parameters, second rank order parameter, localized wobbling diffusion, and curvature-dependent rotational diffusion constants of dipyenylhexatriene (DPH)-labeled PC (DPH-PC) in the host PE/PC matrix were recovered from the measured fluorescence depolarization decays of DPH fluorescence. At approximately 60% PE, abrupt increases in these rotational dynamics parameters were observed, reflecting the onset of packing defects in the host PE/PC matrix. We have demonstrated that rotational dynamics parameters are very sensitive in detecting the onset of curvature-associating packing defects in lipid membranes. In addition, the presence of the D state can be characterized by the enhanced wobbling diffusional motion and order packing of lipid molecules, and by the presence of localized curvatures in the lipid layers. 相似文献
106.
Pei-Wen Chiang SuQing Wang Paul Smithivas Woo-Joo Song Saravanan Ramamoorthy Joseph Hillman Sheryl Puett Margaret L. Van Keuren Eric Crombez Arun Kumar Thomas W. Glover Diane E. Miller Chun-Hui Tsai C.Clare Blackburn Xiao-Ning Chen Zhiguang Sun Jan-Fang Cheng Julie R. Korenberg David M. Kurnit 《Genomics》1996,34(3):328
107.
本文报道了中国马兜铃属12种植物的染色体数目和核型分析,发现并确定了x=8为本属染色体基数之一。本属染色体的共同特征是:小型;以中部(m),近中部(sm)着丝点染色体为主;核型类型为1A,2A,3A,1B,2B;随体一般处在染色体的长臂上。 相似文献
108.
腐殖酸对小麦抗旱性的生理效应 总被引:14,自引:2,他引:12
在干旱气候条件下,喷施腐殖酸钠(HA)、黄腐酸钠(FA)可降低土壤水分损耗,提高小麦叶片持水能力,叶片细胞超氧物歧化酶(SOD)、过氧化氢酶(CAT)活性明显提高,丙二醛(MDA)含量和电解质渗出率明显降低,可减缓叶绿素降解,增强光合速率和光合产物积累,延缓植株衰老,小麦抗旱性增强,千粒重增加. 相似文献
109.
在自生异养条件下,旋扭山绿豆根瘤菌MXDI6菌株的氢酶诱导表达受气相、pH值、镍等因子影响:氢酶表达的最适氧浓度为4%,最适氢浓度为15%,二氧化碳没有明显影响;氢酶表达的pH值以5.0—6.0为宜;0.5μmol/LNiCl2明显促进吸氢活性,但镍浓度大于1μmol/L则抑制吸氢活性. 相似文献
110.
We describe a new immunoassay which combines an immunosorbent assay, Immunogold silver staining and epipolarization microscopy. Our new assay procedure features multiple samples on a single microscope slide, and high sensitivity of epipolarization microscope for detection of silver-enhanced colloidal gold as a final immunoassay product. We call the new immunoassay “on slide immunogold assay” (OSIGA). This new method uses biotinylated antibody and streptavidin-gold reaction with silver enhancement technique. With OSIGA it is possible to investigate 30 samples on a single microscopic slide. Our preliminary studies used 10-20 μ1 samples and detected nanogram quantities of a standardized protein solution. Unlike enzyme linked immunosorbent assay (ELISA), which has a limited time for reading the final color products, the OSIGA specimens can be dried or resin mounted for longer storage and future reference. 相似文献