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401.
Vervet monkeys (Cercopithecus aethiops) at Amboseli, Kenya, give acoustically different alarm calls to different predators. Each alarm evokes contrasting, seemingly adaptive, responses. Animals on the ground respond to leopard alarms by running into trees, to eagle alarms by looking up, and to snake alarms by looking down. In a 14-month field study examining the semantic properties of alarm calls, we played tape-recorded alarms to vervets in the absence of actual predators and filmed the monkeys' responses. Playbacks confirmed observations and showed that (1) alarm length, amplitude and alarmist's age/sex class had little effect on response quality, and (2) context was not a systematic determinant of response. We conclude that vervet alarm calls function to designate different classes of external danger. 相似文献
402.
Pathogenesis of hemodynamic pulmonary edema in excised dog lungs 总被引:1,自引:0,他引:1
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Adhesion of phospholipid vesicles to Chinese hamster fibroblasts: Role of cell surface proteins 下载免费PDF全文
The adhesion of artificially generated lipid membrane vesicles to Chinese hamster V79 fibroblasts in suspension was used as a model system for studying membrane interactions. Below their gel-liquid crystalline phase transition temperature, vesicles comprised of dipalmitoyl lecithin (DPL) or dimyristoyl lecithin (DML) absorbed to the surfaces of EDTA- dissociated cells. These adherent vesicles could not be removed by repeated washings of the treated cells but could be released into the medium by treatment with trypsin. EM autoradiographic studies of cells treated with[(3)H]DML or [(3)H]DPL vesicles showed that most of the radioactive lipids were confined to the cell periphery. Scanning electron microscopy and fluorescence microscopy further confirmed the presence of adherent vesicles at the cell surface. Adhesion of DML or DPL vesicles to EDTA-dissociated cells modified the lactoperoxidase-catalyzed iodination pattern of the cell surface proteins; the inhibition of labeling of two proteins with an approximately 60,000- dalton mol wt was particularly evident. Incubation of cells wit h (3)H-lipid vesicles followed by sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis showed that some of the (3)H-lipid migrated preferentially with these approximately 60,000-mol wt proteins. Studies of the temperature dependence of vesicle uptake and subsequent release by trypsin showed that DML or DPL vesicle adhesion to EDTA- dissociated cells increased with decreasing temperatures. In contrast, cells trypsinized before incubation with vesicles showed practically no temperature dependence of vesicle uptake. These results suggest two pathways for adhesion of lipid vesicles to the cell surface-a temperature-sensitive one involving cell surface proteins, and a temperature-independent one. These findings are discussed in terms of current models for cell-cell interactions. 相似文献