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61.
Cheney F. W.; Bishop M. J.; Chi E. Y.; Eisenstein B. L. 《Journal of applied physiology》1987,62(4):1690-1697
We studied the effects of regional alveolar hypoxia on permeability pulmonary edema formation. Anesthetized dogs had a bronchial divider placed so that the left lower lobe (LLL) could be ventilated with a hypoxic gas mixture (HGM) while the right lung was continuously ventilated with 100% O2. Bilateral permeability edema was induced with 0.05 ml/kg oleic acid and after 4 h of LLL ventilation with an HGM (n = 9) LLL gross weight was 161 +/- 13 (SE) g compared with 204 +/- 13 (SE) g (P less than 0.05) in the right lower lobe (RLL). Bloodless lobar water and dry weight were also significantly lower in the LLL as compared with the RLL of the study animals. In seven control animals in which the LLL fractional inspired concentration of O2 (FIO2) was 1.0 during permeability edema, there were no differences in gravimetric variables between LLL and RLL. In eight additional animals, pulmonary capillary pressure (Pc), measured by simultaneous occlusion of left pulmonary artery and vein, was not significantly different between LLL FIO2 of 1.0 and 0.05 either before or after pulmonary edema. We conclude that, in the presence of permeability pulmonary edema, regional alveolar hypoxia causes reduction in edema formation. The decreased edema formation during alveolar hypoxia is not due to a reduction in Pc. 相似文献
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Cheney F. W.; Bishop M. J.; Eisenstein B. L.; Artman L. D. 《Journal of applied physiology》1987,62(2):776-780
We studied the effects of regional hypoxic pulmonary vasoconstriction (HPV) on lobar flow diversion in the presence of hydrostatic pulmonary edema. Ten anesthetized dogs with the left lower lobe (LLL) suspended in a net for continuous weighing were ventilated with a bronchial divider so the LLL could be ventilated with either 100% O2 or a hypoxic gas mixture (90% N2-5% CO2-5% O2). A balloon was inflated in the left atrium until hydrostatic pulmonary edema occurred, as evidenced by a continuous increase in LLL weight. Left lower lobe flow (QLLL) was measured by electromagnetic flow meter and cardiac output (QT) by thermal dilution. At a left atrial pressure of 30 +/- 5 mmHg, ventilation of the LLL with the hypoxic gas mixture caused QLLL/QT to decrease from 17 +/- 4 to 11 +/- 3% (P less than 0.05), pulmonary arterial pressure to increase from 35 +/- 5 to 37 +/- 6 mmHg (P less than 0.05), and no significant change in rate of LLL weight gain. Gravimetric confirmation of our results was provided by experiments in four animals where the LLL was ventilated with an hypoxic gas mixture for 2 h while the right lung was ventilated with 100% O2. In these animals there was no difference in bloodless lung water between the LLL and right lower lobe. We conclude that in the presence of left atrial pressures high enough to cause hydrostatic pulmonary edema, HPV causes significant flow diversion from an hypoxic lobe but the decrease in flow does not affect edema formation. 相似文献
63.
One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae 总被引:1,自引:0,他引:1
Peter M. Bradley Donald P. Cheney Naotsune Saga 《Plant Cell, Tissue and Organ Culture》1988,12(1):55-60
A simple and rapid method for obtaining axenic plant material has been devised where antibiotic-containing paper disks are applied to nutrient agarose or agar plates upon which small pieces of plant have been spread. By applying multiple disks to the agarose plate, susceptibility to a large number of antibiotics can be tested simultaneously. Clear zones are produced around those disks where contaminating bacteria are susceptible. Plant pieces are then removed from the clear zones and separately tested for sterility to identify the axenic pieces. The method has been successfully applied to multicellular marine algae (e.g.Enteromorpha, Porphyra, laminaria, Gracilaria andAgardhiella). Pieces fromAgardhiella plants survive better on nutrient medium solidified with agarose when -naphthaleneacetic acid and zeatin are present in the medium. 相似文献
64.
A feline large granular lymphoma and its derived cell line 总被引:2,自引:0,他引:2
Carolyn M. Cheney Jennifer L. Rojko Gary J. Kociba Maxey L. Wellman Stephen P. Di Bartola Louis J. Rezanka Lisa Forman Lawrence E. Mathes 《In vitro cellular & developmental biology. Plant》1990,26(5):455-463
Summary A lymphoma cell line (MCC) was derived from an abdominal mass from a 13-yr-old castrated male cat. The cells resemble natural
killer precursor cells, have membrane-bound granules, and are positive for chloroacetate esterase, α-naphthyl butyrate esterase,
and tartrate-resistant acid phosphatase activities. The MCC cells are negative for rearranged feline T-cell receptor genes,
negative for feline T-cytotoxic antigen, Ia, and surface μ, τ, and lambda chains and do not form E-rosettes. The MCC cell
line is negative for the feline leukemia virus (FeLV); e.g., negative for exogenous FeLV (exU3) sequences, negative for cytoplasmic
and surface FeLV major core protein of 27 000 daltons (p27) by indirect, immunofluorescence assay, negative for helper FeLV
by clone 81 assay, and negative for release of soluble FeLV p27 by enzyme-linked immunosorbent assay. Electron microscopy
reveals budding type C retrovirus particles and MCC cells react with anti-RD-114 (anti-endogenous feline retrovirus) reference
serum. After in vitro infection, MCC replicate FeLV readily, but replication is noncytopathic.
This project has been funded, at least in part, with funds from the U.S. Department of Health and Human services under grants
AI 25722, DK41939, and CA 35742 and contract AI-62525. 相似文献
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Sheldon Cheney 《Arts Education Policy Review》2013,114(3):3-4
Profound differences exist between the ways in which arts educators and artists personally value the arts and the rationales offered via arts advocacy campaigns for public arts support. The author argues that those discrepancies carry grave consequences for K-university arts education. The author describes means by which to better reconcile valuing the arts for their intrinsic qualities with intense political pressure to justify arts education in terms of its alleged ability to improve students' math and reading skills and to address concerns of social justice and economic development. This article was adapted from a keynote speech given by the author on 13 October 2006 for the 62nd annual meeting of The National Association of Schools of Art and Design. 相似文献
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