全文获取类型
收费全文 | 164篇 |
免费 | 2篇 |
专业分类
166篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 4篇 |
2015年 | 2篇 |
2014年 | 5篇 |
2013年 | 7篇 |
2012年 | 6篇 |
2011年 | 9篇 |
2010年 | 7篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 18篇 |
2006年 | 6篇 |
2005年 | 7篇 |
2004年 | 8篇 |
2003年 | 5篇 |
2002年 | 9篇 |
2001年 | 7篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有166条查询结果,搜索用时 9 毫秒
81.
An. Kh. Baymiev R. S. Yamidanov R. T. Matniyazov D. K. Blagova Al. Kh. Baymiev A. V. Chemeris 《Molecular Biology》2011,45(6):904-910
A series of expression vectors containing TurboGFP and TurboRFP genes of fluorescent proteins under the control of the T5 phage constitutive promoter was created for a vital staining of nodule bacteria. These vectors were either obtained using the broad host range pBBRI replicon for labeling of strains, where a marker gene was expressed from a transformed plasmid, or they were prepared using the pRL765 gfp plasmid for labeling of strains via the introduction of genes of fluorescent proteins into the bacterial chromosome. Transformation was shown to be the most convenient method of transfer of constructions into cells of nodule bacteria, as there exists the possibility of spontaneous plasmid mobilization and, consequently, its transition from cells of labeled strains into other soil bacteria if the mob locus is present in vectors needed for conjugation. Fluorescent labeled strains of Rhizobium sp., Mesorhizobium sp., Ensifer (Sinorhizobium) sp., Bradyrhizobium sp., Phyllobacterium sp., and Agrobacterium sp. were prepared using the obtained vector constructions. The suitability of the obtained strains for both in vivo and in vitro experiments was demonstrated. 相似文献
82.
83.
A. V. Popinako M. Yu. Antonov A. S. Chemeris K. V. Shaitan O. S. Sokolova 《Biophysics》2017,62(6):885-891
The Arp2/3 complex is one of the main regulators of the actin cytoskeleton and a basic molecular machine that nucleates the branched actin filaments. In this work, we studied the interaction of the Arp2/3 complex with its inhibitor, arpin, and revealed the amino-acid residues that are responsible for complex formation. The free-energy calculation for arpin binding to the Arp2/3 complex was performed using umbrella sampling. It has been shown that the dissociation constant of the Arp2/3–arpin complex is higher on average than that of Arp2/3 complexes with other inhibitors. Two arpin binding sites with different affinities were identified on the surface of the Arp2/3 complex. The mechanism of the inhibition of the Arp2/3 complex by arpin is discussed. 相似文献
84.
Upon inducing acute local inflammation in the mouse hindpaw with zymosan, exposure to pulsed microwaves (35.27 GHz, peak power 20 kW, pulse duration 400–600 ns, repetition rate 5–500 s?1) could attenuate the exudative edema and local hyperthermia by 20%; in dynamics and scale these effects were similar to those of 3 μg/g Diclofenac. The anti-inflammatory effect increased roughly linearly with pulse duration at the same repetition rate, and showed a threshold dependence on average power flux density at fixed pulse duration. The dependence on the duration of exposure at fixed emission parameters was bell-shaped both in total irradiation and in local limb irradiation. 相似文献
85.
Administration of 1,2-dimethylhydrazine (DMH) produced 83% (15/18) of renal capsule angiosarcomas in CBA mice. Castration that preceded the DMH-treatment reduced tumor incidence to 7% (2/29). Simultaneous administration of DMH and testosterone propionate (TP) to castrated males restored the tumor frequency (100%, 24/24). Castrated males that received TP after the cessation of the DMH treatment developed tumors in 10% (3/31). Combined treatment of castrated females with DMH and TP resulted in the development of angiosarcomas in 92% animals (22/24). It is concluded that TP enhances the stage of sarcomogenesis initiation induced by DMH. 相似文献
86.
The nucleotide sequences of 280-360-bp domains of lectin genes from 20 legume species belonging to 17 genera have been determined. A computer analysis of the sequences has been performed with the LASERGENE package. Based on this analysis, we constructed the phylogenetic tree of the lectins, which reflects their phylogenetic and evolutionary relationships, and predicted the amino-acid sequences of the corresponding protein domains. Features of the structure of the hydrocarbon-binding lectin domains were elucidated in some species of legume genera from the temperate climatic zone. The domains were highly variable and contained the consensus sequence AspTrePheXxxAsxXxxXxxTrpAspProXxxXxxIns/DelArgHis bearing the bulk of amino acid replacements, insertions, and deletions. An association between legume groups (including species from different genera and tribes) symbiotic with the same rhizobium species and the similarity between the hydrocarbon-binding domains of lectins from these plants was found. 相似文献
87.
Grinevich A. A. Gharamyan B. G. Chemeris N. K. 《Doklady. Biochemistry and biophysics》2022,504(1):118-122
Doklady Biochemistry and Biophysics - Peripheral blood flow analysis is used for noninvasive assessment of the cardiovascular system. The aim was to investigate the possibility of applying... 相似文献
88.
Biophysics - Abstract—The experimentally revealed high phase coherence between low-frequency oscillations in the cutaneous blood perfusion signal at the contralateral skin sites indicates the... 相似文献
89.
Al. Kh. Baimiev An. Kh. Baimiev I. I. Gubaidullin O. L. Kulikova A. V. Chemeris 《Russian Journal of Genetics》2007,43(5):587-590
The population genetic diversity and phylogeny of the bacteria entering the symbiosis with sainfoin that grows on the Chesnokovskaya Mountain, Ufaregion, Republic of Bashkortostan, have been studied. RAPD analysis of DNA polymorphism of the microbial strains grown from the nodules of 20 plants using several random primers detected a high degree of genetic homogeneity in their population as compared with the populations of rhizobia of other leguminous plants growing at the same site. Sequencing of 16S rRNA genes of the three most different samples have demonstrated that these genes were identical and display 99.9% homology with the sequence of Phyllobacterium trifolii 16S rRNA gene. 相似文献
90.
YK Onno Teng Gillian Wheater Vanessa E Hogan Philip Stocks EW Nivine Levarht Tom WJ Huizinga Rene EM Toes Jacob M van Laar 《Arthritis research & therapy》2012,14(2):R57