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151.
Early toxic effects of heavy metals (HMs) Zn, Co, and Cd at concentrations from 0.01 to 100 mM on photosynthetic activity of the green algaChlorella pyrenoidosaChick S-39 were studied. The early effect of HMs was manifested as a rapid (within 0.5–2 h) reduction of photoinduced oxygen release by the algal cells. The suppressed relative yield of variable chlorophyll fluorescence (F
V
/F
m
) by HMs as well as its dynamics inC. pyrenoidosaprovided evidence for rapid inactivation of photosystem II (PS II). Analysis of the induction curve of delayed chlorophyll fluorescence inChlorellacells suggested that the early toxic effects of Zn, Co, and Cd at the above concentrations manifested itself not only in inhibited electron transport in PS II, but also in reduced energization of photosynthetic membranes. Hence, the early toxic effect of Zn, Co, and Cd was primarily related to the decreased efficiency of the light reactions of photosynthesis, which subsequently resulted in reduced productivity of the alga. 相似文献
152.
B. R. Kuluev A. V. Knyazev B. N. Postrigan A. V. Chemeris 《Russian Journal of Genetics》2014,50(1):37-44
Transgenic tobacco plants expressing the fragments of the ARGOS and NtEXPA4 genes in antisense orientation have been created. Eleven lines of transgenic plants were investigated and five of them were characterized by a decrease in the sizes of the leaves and flowers as compared to control. Stem sizes decreased when only the NtEXPA4 gene fragment was used. The organ size of the experimental plants decreased because of a reduction in the level of both cell division and cell expansion. Two lines of transgenic tobacco plants expressing the part of the ARGOS gene in antisense orientation were characterized by a reduction in the level of the NtEXPA1 and NtEXPA4 gene expression. 相似文献
153.
B. R. Kuluev A. V. Knyazev Y. M. Nikonorov A. V. Chemeris 《Russian Journal of Genetics》2014,50(5):489-497
The tobacco plant genes NtEXPA1 and NtEXPA4 encode the α-expansin proteins involved in the regulation of cell growth and extension. We examined the levels of expression of these genes in various plant organs and under the effect of exogenous phytohormones. The highest level of NtEXPA1 expression were registered in the terminal bud and in the young growing leaves and flowers. NtEXPA1 expression ceased once the leaves stopped growing. The NtEXPA4 gene showed a similar expression profile, except for higher levels of mRNA in the leaves. In young leaves located near the terminal bud, high levels of NtEXPA1 and NtEXPA4 are induced by auxins. In the lower leaves, expansin expression is differentially regulated by brassinosteroids, which inhibit NtEXPA1 and upregulate NtEXPA4. We further showed that expression of the transgenic ARGOS-LIKE protein results in upregulation of NtEXPA1 and a reduction in the NtEXPA4 mRNA. In turn, overexpression of NtEXPA1 resulted in an increased size of the leaves and stems because of the larger size of the individual cells. 相似文献
154.
Using the alkaline single cell gel electrophoresis technique (comet assay), changes in chromatin structure of peripheral blood
leukocytes and peritoneal neutrophils have been studied in mice exposed to low-intensity extremely high-frequency electromagnetic
radiation (42.2 GHz, 0.1 mW/cm2, 20 min at 1 h after induction of inflammation) against the background of the systemic inflammatory process. It was revealed
that the exposure of mice with the developing inflammation leads to a pronounced decrease in the level of DNA damage to peripheral
blood leukocytes and peritoneal neutrophils. It is supposed that the changes in the chromatin structure of lymphoid cells
have a genoprotective character in the inflammatory process and can underlie the mechanisms of realization of antiinflammatory
effects of the electromagnetic radiation. 相似文献
155.
156.
An. Kh. Baymiev R. S. Yamidanov R. T. Matniyazov D. K. Blagova Al. Kh. Baymiev A. V. Chemeris 《Molecular Biology》2011,45(6):904-910
A series of expression vectors containing TurboGFP and TurboRFP genes of fluorescent proteins under the control of the T5
phage constitutive promoter was created for a vital staining of nodule bacteria. These vectors were either obtained using
the broad host range pBBRI replicon for labeling of strains, where a marker gene was expressed from a transformed plasmid,
or they were prepared using the pRL765 gfp plasmid for labeling of strains via the introduction of genes of fluorescent proteins
into the bacterial chromosome. Transformation was shown to be the most convenient method of transfer of constructions into
cells of nodule bacteria, as there exists the possibility of spontaneous plasmid mobilization and, consequently, its transition
from cells of labeled strains into other soil bacteria if the mob locus is present in vectors needed for conjugation. Fluorescent labeled strains of Rhizobium sp., Mesorhizobium sp., Ensifer (Sinorhizobium) sp., Bradyrhizobium sp., Phyllobacterium sp., and Agrobacterium sp. were prepared using the obtained vector constructions. The suitability of the obtained strains for both in vivo and in
vitro experiments was demonstrated. 相似文献
157.
158.
Garafutdinov R. R. Sakhabutdinova A. R. Gilvanov A. R. Chemeris A. V. 《Russian Journal of Bioorganic Chemistry》2021,47(6):1172-1189
Russian Journal of Bioorganic Chemistry - Detection and quantification of biotargets are important analytical tasks, which are solved using a wide range of various methods. In recent years, methods... 相似文献
159.
160.