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101.
Zilya R. Vershinina Andrei K. Baymiev Darya K. Blagova Olga V. Chubukova Aleksei K. Baymiev Aleksei V. Chemeris 《Symbiosis (Philadelphia, Pa.)》2012,56(1):25-33
Rhizobia have the ability to increase growth of non-legume plants due to the production of phytohormones and protection of
plant from diseases and pathogens. However, the practical use of these beneficial bacteria sometimes fails because of their
inability to effectively colonize rhizoplane and rhizosphere of inoculated plants. We chose the legume lectins as a factor
that allows plants to form associative symbiosis with rhizobia. To test the fact that transgenic tobacco, tomato and rape
roots with pea lectin gene may affect specific interaction with rhizobia, transgenic roots have been artificially inoculated
by fluorescently-labeled pea rhizobia R. leguminosarum and east galega rhizobia Rhizobium galega. Microscopic and microbiological tests have shown that the number of adhered R. leguminosarum onto tobacco, rape and tomato roots which transformed with pea lectin gene is higher in comparison with the control, but
no such effect through inoculation of these plants with R. galegae has been found. This confirms the interaction of R. leguminosarum with pea lectin at the surface of transformed roots. Undoubtedly, the improvement of recognition and attachment processes
by using lectins can lead to the achievement of a stable associative relationship between non-symbiotic plants and rhizobia. 相似文献
102.
103.
Baĭmiev AKh Iamidanov RS Matniiazov RT Blagova DK Baĭmiev AKh Chemeris AV 《Molekuliarnaia biologiia》2011,45(6):984-991
A series of expression vectors containing genes of fluorescent proteins TurboGFP and TurboRFP under the phage T5 constitutive promoter regulation, intended for lifetime marking of nodule bacteria is created: a series of vectors based on a broad-host-range replicon BBRI, for marking strains with an expression of reporter gene from a transformed plasmid and a series of vectors based on a plasmid pRL765gfp for marking strains by introduction genes of fluorescent proteins in a bacterial chromosome. It was shown that transformation is the most preferable method of constructions transfer in nodule bacteria cells, as in the presence of mob locus in the vectors necessary for conjugation, exists the possibility of occasional plasmid mobilization and its transition from marked strain cells in other soil bacteria. With application of the created vector constructions we obtained fluorescent tagged strains of Rhizobium sp., Mesorhizobium sp., Ensifer (Sinorhizobium) sp., Bradyrhizobium sp., Phyllobacterium sp., Agrobacterium sp. Also their suitability for experiments in vivo and in vitro is shown. 相似文献
104.
B. R. Kuluev A. V. Knyazev A. A. Iljassowa A. V. Chemeris 《Russian Journal of Plant Physiology》2011,58(3):507-515
The auxin-inducible gene ARGOS from Arabidopsis thaliana is expressed in growing tissues and controls the plant organ size by regulating cell proliferation and meristematic competence.
The promoter of the dahlia (Dahlia pinnata Cav.) mosaic virus (DMV) resembles the well-known cauliflower mosaic virus 35S promoter but shows a higher activity in transgenic
tobacco plants (Nicotiana tabacum L.). We obtained transgenic tobacco plants expressing the Arabidopsis ARGOS gene under the control of the DMV promoter. Several of the T0 generation plants exhibited an accelerated transition to flowering,
a slight increase in flower size, and a significant increase in the leaf size. The T1 transgenic plants were characterized
by faster growth, the increased leaf size, and somewhat enlarged flowers as compared with control plants. These phenotypic
traits, as well as stability and inheritance of the transgene were demonstrated also in T2 transgenic plants. 相似文献
105.
Li Tai Fang Pegah Tootoonchi Afshar Aparna Chhibber Marghoob Mohiyuddin Yu Fan John C. Mu Greg Gibeling Sharon Barr Narges Bani Asadi Mark B. Gerstein Daniel C. Koboldt Wenyi Wang Wing H. Wong Hugo YK Lam 《Genome biology》2015,16(1)
SomaticSeq is an accurate somatic mutation detection pipeline implementing a stochastic boosting algorithm to produce highly accurate somatic mutation calls for both single nucleotide variants and small insertions and deletions. The workflow currently incorporates five state-of-the-art somatic mutation callers, and extracts over 70 individual genomic and sequencing features for each candidate site. A training set is provided to an adaptively boosted decision tree learner to create a classifier for predicting mutation statuses. We validate our results with both synthetic and real data. We report that SomaticSeq is able to achieve better overall accuracy than any individual tool incorporated.
Electronic supplementary material
The online version of this article (doi:10.1186/s13059-015-0758-2) contains supplementary material, which is available to authorized users. 相似文献106.
107.
Tankanag AV Tikhonova IV Chemeris NK 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2008,94(3):260-266
Study of peripheral microhaemodynamics was carried out with laser Doppler flowmetry in healthy volunteers of different age groups. The ageing changes in the state of the skin peripheral blood flow, in the functioning of separate links and regulatory systems ofmicrovascular bed have been estimated in terms of relative entropy and fractal dimension values. The revealed significant age-dependent decrease of relative entropy values in the respiratory rhythm ranges, the neurogenic and myogenic activities yielded some evidence concerning the reduction of the microcirculation system chaotic changes within these frequency ranges during the ageing. The significant increase of fractal dimension values in the ranges of cardio-rhythm and the endothelial activity in the oldest group with the mean age of 77 years indicated that the structural complexity of the oscillations in these frequency ranges increased during ageing. 相似文献
108.
Chemeris Yu. K. Korol'kov N. S. Seifullina N. Kh. Rubin A. B. 《Russian Journal of Plant Physiology》2004,51(3):287-293
A prolonged (20–24 h) dark incubation of Chlorella pyrenoidosa algae at 37–38° did not diminish the relative yield of the variable chlorophyll fluorescence (F
v/F
m) and enhanced the relative contribution of the slow phase (sF
v) to the kinetics of F
v increase. Iodoacetamide, a nonmetabolized glucose analog, 2-deoxyglucose (2-DG), an inhibitor of protein synthesis, cycloheximide, and a decrease in the temperature of dark incubation to 18–20° prevented this sF
v increase. Both the illumination of dark-incubated cells and the addition of 2-DG in darkness restored the initial level of sF
v. In the light-grown chlorella cells, the relative contribution of sF
v reversibly declined with lowering light intensity and increased when 2 was excluded from the bubbling mixture. The authors presume that the slow phase in the kinetics of F
v increase is related to the functioning of the fraction of the photosystem II complexes with a destabilized primary quinone acceptor of electron, and the content of these complexes in the cell depends on the plastoquinone redox state. 相似文献
109.
Using experimental and theoretical methods of dosimetry, the energy absorption of extremely high-frequency electromagnetic radiation (EHF EMR) in the skin of laboratory rats was analyzed. Specific absorption rate (SAR) in the skin was determined on the basis of both microthermometric measurements of initial rates of temperature rise in rat skin induced by the exposure and microcalorimetric measurements of specific heat of the skin. Theoretical calculations of SAR in the skin were performed with consideration for dielectric parameters of rat skin obtained from the measurements of the standing wave ratio upon reflection of electromagnetic waves from the skin surface and for the effective area of stationary overheating measured by infrared thermography. A numerical method was developed to determine electromagnetic wave energy reflected, absorbed, and transmitted in the model of flat layers. The algorithm of the method was realized in a computer program and used to calculate SAR in the skin on the basis of the complex dielectric constant of rat skin. The SAR values obtained from experimental measurements, theoretical calculations and numerical analysis are in good mutual correspondence and make about 220-280 W/kg at a frequency of 42.25 GHz and a power of 20 mW at the radiator output. The results obtained can be used for dosimetric supply of biomedical experiments on studying the physicochemical mechanisms of the biological effects of EHF EMR. 相似文献
110.
Using the method of enhanced chemiluminescence in a peroxidase-luminol-p-iodophenol system, we found the formation of reactive oxygen species (in equivalent of hydrogen peroxide concentration) in 1 mM phosphate buffer under the exposure to high peak-power pulsed electromagnetic radiation of extremely high frequencies (37 GHz, peak power 20 kW, pulse width 400 ns, repetition rate 500 Hz). The results obtained show that the formation of hydrogen peroxide in aqueous solutions under the action of electromagnetic radiation is the result of the summary influence of heat and thermoacoustic waves excited in the solutions. 相似文献