Fragile X expression in thymidine-prototrophic and auxotrophic human-mouse somatic cell hybrids under low and high thymidylate stress conditions

Expression of the fragile X site fra(X)(q27.3) was studied in thymidine-prototrophic and auxotrophic human-mouse somatic cell hybrids. In these cells, low thymidylate stress, achieved by 5-fluoro-2'-deoxyuridine (FdU) treatment and by limiting the exogenous supply of thymidine (dT), induced fragile X expression. High thymidylate stress, produced by supplying excess amounts of dT, was also effective in inducing fragile X expression, even in a hybrid clone that retained a fragile X chromosome as the only human chromosome; addition of deoxycytidine (dC) completely abolished this effect. In contrast, 5-bromo-2'-deoxyuridine (BrdU) did not induce fragile X expression. Cell-cycle analysis of BrdU-deprived thymidine-auxotrophic hybrid cells indicated that one round of DNA replication under thymidylate stress conditions is sufficient for fragile X expression. Our results suggest that the expression is an intrinsic property of the fragile site itself, which is believed to be composed of replicon clusters with pyrimidine-rich DNA sequence(s).     

Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa

Summary H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head and the mid-piece region of the flagellum. In addition, caput spermatozoa also exhibit intense immunoperoxidase staining of the cytoplasmic droplet which is situated on the flagellum at the base of the head. Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a malespecific protein with an estimated molecular weight of 25,000–27,000.     

Avian thrombocyte thrombospondin

A high molecular weight glycoprotein (450,000) was obtained from thrombin-treated duck thrombocytes by barium citrate adsorption technique followed by heparin-agarose affinity chromatography. Amino acid composition (high number of acidic amino acids and cystine) as well as carbohydrate contents (1.3 per cent hexosamine, 0.9 per cent sialic acid and 1.5 per cent hexose) showed similarity to mammalian platelet thrombospondin.     

Sexual dimorphism in scales of marbled flounder Pseudopleuronectes yokohamae (Pleuronectiformes: Pleuronectidae), with comments on the relevance to their spawning behaviour

Variation of scales on the blind side of Pseudopleuronectes yokohamae in relation to sex, maturity and body size was examined. Immature males often have cycloid scales, while mature males have mostly ctenoid scales. Large females also often have ctenoid scales (but with fewer spines compared with males), and small females have mostly cycloid scales. The number of spines (ctenii) on the blind‐side scale increases with body size in both sexes, indicating an ontogenetic change in scale morphology. As P. yokohamae spawn demersal eggs with males positioning themselves above the females on the ocular side, it is hypothesized that ctenoid scales on the blind side in mature males function for maintaining contact with females during spawning.     

NUTRITION OF TRIDACHIA CRISPATA (MORCH) (SACOGLOSSA)

Autotrophic nutrition plays an important role in adult Tridachiacrispata in the western Atlantic Ocean and results from an endosymbioticassociation between the sacoglossan and the kleptoplastids ofChlorophyta. A series of living specimens 7-80 mm in length was studied inthe laboratory. After a body length of 10 mm has been attained,the parapodial lobes of the two sides meet and fuse. Microscopicexamination of faecal strings of specimens undergoing this transitionrevealed that algal remains were produced only by juvenilesless than 13 mm long. Larger specimens defaecated only rarelyand the diminutive faecal strings contained no recognizablealgal debris. This transition probably parallels the physiologicaltransition between juvenile heterotrophic nutrition and adultautotrophic nutrition. Microscopic examination of the radulae of transitional specimensshowed no diminution of the size, number or morphology of theteeth. This indicates that in an emergency heterotrophic nutritionmay be resumed. Growth rates of Tridachia were measured in the laboratory, withand without light and the provision of algal foods. Unfed animalslost weight rather rapidly in captivity, whereas those fed uponsuitable algae (Halimeda discoidea, Caulerpa verticillata, C.racemosa and Chaetomorpha sp) did not.Caulerpa sertularioideswas suitable for food for a limited time, but proved toxic afterabout a week. Of the other species tested, Caulerpa verticillatawas the least suitable, while Halimeda, Chaetomorpha and Caulerparacemosa were the best. Ironically, C. verticillata was theonly one of the algal species tested which is known to be takenin the natural diet in Floridean waters. These experiments confirmedthat heterotrophic feeding will be resumed in Tridachia if theculture-conditions do not favour autotrophic nutrition.     

Ultrastructural study of enzymes in reactive astrocytes: Clarification of astrocytic activity

Summary Enzymes in reactive astrocytes of the corebral cortex were examined at the ultrastructural level in an attempt to resolve some conflicting aspects of astrocytic activity. Correlations between morphological and enzyme changes after injury established that the apparent increase in oxidative enzyme activity was exclusively mitochondrial and not an artefactual reaction product resulting from anoxic cellular damage. Pronounced glucose-6-phosphatase activity within cisternae of an increased amount of the granular endoplasnie reticulum was related to increased glycogen. Further evidence from acid phosphatase activity indieated that astrocytes played a minimal role in phagocytosis.     

Partial and total iodide organification defect in different sibships in a kindred.

Identical twins with goiter but without intellectual retardation and with slightly delayed bone age were found to have defective iodide organification as demonstrated by incomplete perchlorate discharge tests. They are grandnieces of a normal member of a sibship which included four children with severe retardation and complete thyroid iodide organification defect. The parents and grandparents are not consanguine. Possible explanations are considered for the problem of why the disorder is manifest completely in one sibship and only partially in the other.