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111.
Takai R Kaneda T Isogai A Takayama S Che FS 《Bioscience, biotechnology, and biochemistry》2007,71(2):590-593
We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms. 相似文献
112.
Che FY Zhang X Berezniuk I Callaway M Lim J Fricker LD 《Journal of proteome research》2007,6(12):4667-4676
Sample preparation for neuropeptidomic studies is a critical issue since protein degradation can produce high levels of peptides that obscure the endogenous neuropeptides. We compared different extraction conditions for the recovery of neuropeptides and the formation of protein breakdown fragments from mouse hypothalami. Sonication and heating in water (70 degrees C for 20 min) followed by cold acid and centrifugation enabled the efficient extraction of many neuropeptides without the formation of protein degradation fragments seen with hot acid extractions. The hot water/cold acid extraction procedure resulted in the reproducible recovery of many hypothalamic peptides, including several novel peptides. 相似文献
113.
Wang Linwen Wang Hui Wang Yuncai Che Yue Ge Zhiwei Mao Lingfeng 《Biodiversity and Conservation》2022,31(7):1771-1796
Biodiversity and Conservation - As a form of green infrastructure, green roofs can enhance urban biodiversity by providing complex vegetation structures, supplying increased foraging and roosting... 相似文献
114.
115.
Markus Schueler Daniela?A. Braun Gayathri Chandrasekar Heon?Yung Gee Timothy?D. Klasson Jan Halbritter Andrea Bieder Jonathan?D. Porath Rannar Airik Weibin Zhou Joseph?J. LoTurco Alicia Che Edgar?A. Otto Detlef B?ckenhauer Neil?J. Sebire Tomas Honzik Peter?C. Harris Sarah?J. Koon Meral Gunay-Aygun Sophie Saunier Klaus Zerres Nadina?Ortiz Bruechle Joost?P.H. Drenth Laurence Pelletier Isabel Tapia-Páez Richard?P. Lifton Rachel?H. Giles Juha Kere Friedhelm Hildebrandt 《American journal of human genetics》2015,96(1):81-92
Nephronophthisis-related ciliopathies (NPHP-RC) are recessive diseases characterized by renal dysplasia or degeneration. We here identify mutations of DCDC2 as causing a renal-hepatic ciliopathy. DCDC2 localizes to the ciliary axoneme and to mitotic spindle fibers in a cell-cycle-dependent manner. Knockdown of Dcdc2 in IMCD3 cells disrupts ciliogenesis, which is rescued by wild-type (WT) human DCDC2, but not by constructs that reflect human mutations. We show that DCDC2 interacts with DVL and DCDC2 overexpression inhibits β-catenin-dependent Wnt signaling in an effect additive to Wnt inhibitors. Mutations detected in human NPHP-RC lack these effects. A Wnt inhibitor likewise restores ciliogenesis in 3D IMCD3 cultures, emphasizing the importance of Wnt signaling for renal tubulogenesis. Knockdown of dcdc2 in zebrafish recapitulates NPHP-RC phenotypes, including renal cysts and hydrocephalus, which is rescued by a Wnt inhibitor and by WT, but not by mutant, DCDC2. We thus demonstrate a central role of Wnt signaling in the pathogenesis of NPHP-RC, suggesting an avenue for potential treatment of NPHP-RC. 相似文献
116.
Shengjian Li Yu-He Liang Jennifer Mariano Meredith B. Metzger Daniel K. Stringer Ventzislava A. Hristova Jess Li Paul A. Randazzo Yien Che Tsai Xinhua Ji Allan M. Weissman 《The Journal of biological chemistry》2015,290(51):30225-30239
RING proteins constitute the largest class of E3 ubiquitin ligases. Unlike most RINGs, AO7 (RNF25) binds the E2 ubiquitin-conjugating enzyme, UbcH5B (UBE2D2), with strikingly high affinity. We have defined, by co-crystallization, the distinctive means by which AO7 binds UbcH5B. AO7 contains a structurally unique UbcH5B binding region (U5BR) that is connected by an 11-amino acid linker to its RING domain, forming a clamp surrounding the E2. The U5BR interacts extensively with a region of UbcH5B that is distinct from both the active site and the RING-interacting region, referred to as the backside of the E2. An apparent paradox is that the high-affinity binding of the AO7 clamp to UbcH5B, which is dependent on the U5BR, decreases the rate of ubiquitination. We establish that this is a consequence of blocking the stimulatory, non-covalent, binding of ubiquitin to the backside of UbcH5B. Interestingly, when non-covalent backside ubiquitin binding cannot occur, the AO7 clamp now enhances the rate of ubiquitination. The high-affinity binding of the AO7 clamp to UbcH5B has also allowed for the co-crystallization of previously described and functionally important RING mutants at the RING-E2 interface. We show that mutations having marked effects on function only minimally affect the intermolecular interactions between the AO7 RING and UbcH5B, establishing a high degree of complexity in activation through the RING-E2 interface. 相似文献
117.
Derivative of plant phenolic compound inhibits the type III secretion system of Dickeya dadantii via HrpX/HrpY two‐component signal transduction and Rsm systems
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118.
Xiuna Wang Xiaoling Zhang Ling Liu Meichun Xiang Wenzhao Wang Xiang Sun Yongsheng Che Liangdong Guo Gang Liu Liyun Guo Chengshu Wang Wen-Bing Yin Marc Stadler Xinyu Zhang Xingzhong Liu 《BMC genomics》2015,16(1)
Background
In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics.Results
Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion.Conclusion
The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-014-1190-9) contains supplementary material, which is available to authorized users. 相似文献119.
Wen-liang Xiang Hua-zhong Liang Sen Liu Fan Luo Jie Tang Ming-yuan Li Zhen-ming Che 《World journal of microbiology & biotechnology》2011,27(11):2629-2637
The use of halotolerant phosphate solubilizing bacteria as inoculants to convert insoluble phosphorus of salt-affected soils
to an accessible form is a promising strategy to improve the phosphorus ingestion of plants in salt-affected agriculture.
A total of four aerobic isolates with biggest clear halos on the 10% NaCl NBRIP medium plate containing tricalcium phosphate
were isolated from the rhizospheric soils of native plants growing on the wall of Dagong Ancinet Brine Well, located in Sichuan
of China. And these four isolates were classified to the same strain, named QW10-11, and closely related to Bacillus
megatherium var. phosphaticum DSM 3228 and B. megaterium ATCC 14581 according to their phenotype and 16S rRNA. However, the Molecular evolutionary evidences of 16S-23S rRNA ISR further
suggested that QW10-11, DSM 3228 and ATCC 14581 have respectively fall into the different sub-divisions in intra specific
phylogeny. Strain QW10-11 has significantly better ability of tricalcium phosphate solubilization than that of lecithin solubilization.
When it grows under pH 4.8–8.0, 24–33°C and 5–10% NaCl, it can exhibit the higher values of solubilized tricalcium phosphate
between 59.3 and 71.4 μg ml−1. Furthermore, its tricalcium phosphate solubilizing activity was associated with the release of organic acids. Taken together,
our results indicted that QW10-11 from the rhizospheric soils of halobiot of Dagong Ancinet Brine Well is attractive as efficient
phosphate solubilizing candidates in the salt-affected agriculture. 相似文献
120.