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101.
Relative impact of mate versus pollinator availability on pollen limitation and outcrossing rates in a mass‐flowering species
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C. E. L. Delmas N. Escaravage P‐O. Cheptou O. Charrier S. Ruzafa P. Winterton A. Pornon 《Plant biology (Stuttgart, Germany)》2015,17(1):209-218
Plant mating systems are driven by several pre‐pollination factors, including pollinator availability, mate availability and reproductive traits. We investigated the relative contributions of these factors to pollination and to realized outcrossing rates in the patchily distributed mass‐flowering shrub Rhododendron ferrugineum. We jointly monitored pollen limitation (comparing seed set from intact and pollen‐supplemented flowers), reproductive traits (herkogamy, flower size and autofertility) and mating patterns (progeny array analysis) in 28 natural patches varying in the level of pollinator availability (flower visitation rates) and of mate availability (patch floral display estimated as the total number of inflorescences per patch). Our results showed that patch floral display was the strongest determinant of pollination and of the realized outcrossing rates in this mass‐flowering species. We found an increase in pollen limitation and in outcrossing rates with increasing patch floral display. Reproductive traits were not significantly related to patch floral display, while autofertility was negatively correlated to outcrossing rates. These findings suggest that mate limitation, arising from high flower visitation rates in small plant patches, resulted in low pollen limitation and high selfing rates, while pollinator limitation, arising from low flower visitation rates in large plant patches, resulted in higher pollen limitation and outcrossing rates. Pollinator‐mediated selfing and geitonogamy likely alleviates pollen limitation in the case of reduced mate availability, while reduced pollinator availability (intraspecific competition for pollinator services) may result in the maintenance of high outcrossing rates despite reduced seed production. 相似文献
102.
Ana Victoria Garcia Amelie Charrier Adam Schikora Jean Bigeard Stephanie Pateyron Marie-Ludivine de Tauzia-Moreau Alexandre Evrard Axel Mithofer Marie Laure Martin-Magniette Isabelle Virlogeux-Payant Heribert Hirt 《植物生理与分子生物学学报》2014,(4):657-674
Infections with Salmonella enterica belong to the most prominent causes of food poisoning and infected fruits and vegetables represent important vectors for salmonellosis. Recent evidence indicates that plants recognize S. enterica and raise defense responses. Nonetheless, the molecular mechanisms controlling the interaction of S. enterica with plants are still largely unclear. Here, we show that flagellin from S. enterica represents a prominent pathogenassociated molecular pattern (PAMP) in Arabidopsis thaliana, which induces PAMP-triggered immunity (PTI) via the recognition of the fig22 domain by the receptor kinase FLS2. The Arabidopsis fls2 mutant shows reduced though not abolished PTI activation, indicating that plants rely also on recognition of other S. enterica PAMPs. Interestingly, the S. enterica type III secretion system (T3SS) mutant prgH- induced stronger defense gene expression than wild-type bacteria in Arabidopsis, suggesting that T3SS effectors are involved in defense suppression. Furthermore, we observe that S. enterica strains show variation in the fig22 epitope, which results in proteins with reduced PTI-inducing activity. Altogether, these results show that S. enterica activates PTI in Arabidopsis and suggest that, in order to accomplish plant colonization, S. enterica evolved strategies to avoid or suppress PTI. 相似文献
103.
Pascal C. Musoli Christian Cilas David Pot Agnes Nabaggala Saleh Nakendo James Pande André Charrier Thierry Leroy Daniel Bieysse 《Tree Genetics & Genomes》2013,9(2):351-360
Knowledge on heritability is essential for selecting varieties resistant against coffee wilt disease caused by Fusarium xylarioides, which is currently devastating coffee in East and Central Africa. Variability of the resistance against coffee wilt disease in Coffea canephora and its inheritance were investigated in three experiments corresponding to a clonal trial involving 20 clones, a 10-parent partial diallel progeny, and a half-sib progeny test. There were significant quantitative genetic variations among the clones and progenies, suggesting polygenic control of the resistance. Around 50–65 % tree mortality was the optimal disease level for calculating heritability and genetic gains. General and specific combining abilities calculated within the optimal disease range for partial diallel analysis were significant. Broad-sense heritability for the same analysis and same disease range was moderate (0.329), and corresponding narrow-sense heritability was low (0.112). Broad-sense heritability from clones in the field at the same disease range was also moderate (0.333). Narrow-sense heritability from regression of half-sib progeny means onto parent means in the field and screen house was 0.183 and 0.369, respectively. Selecting tolerant clones for improvement against the disease is possible, and genetic gains are possible by using tolerant parents in breeding programs. 相似文献
104.
Croft Darin A. Flynn John J. Wyss André R. Charrier Reynaldo Anaya Federico 《Journal of Mammalian Evolution》2021,28(4):1205-1236
Journal of Mammalian Evolution - The fossil record of chinchillid rodents (Hystricomorpha: Caviomorpha) begins in the early Miocene. However, nearly all remains have thus far been limited to the... 相似文献
105.
Florianne Marandel Grgory Charrier Jean‐Baptiste Lamy Sabrina Le Cam Pascal Lorance Verena M. Trenkel 《Ecology and evolution》2020,10(4):1929-1937
Effective population size (Ne) is a key parameter of population genetics. However, Ne remains challenging to estimate for natural populations as several factors are likely to bias estimates. These factors include sampling design, sequencing method, and data filtering. One issue inherent to the restriction site‐associated DNA sequencing (RADseq) protocol is missing data and SNP selection criteria (e.g., minimum minor allele frequency, number of SNPs). To evaluate the potential impact of SNP selection criteria on Ne estimates (Linkage Disequilibrium method) we used RADseq data for a nonmodel species, the thornback ray. In this data set, the inbreeding coefficient FIS was positively correlated with the amount of missing data, implying data were missing nonrandomly. The precision of Neestimates decreased with the number of SNPs. Mean Ne estimates (averaged across 50 random data sets with2000 SNPs) ranged between 237 and 1784. Increasing the percentage of missing data from 25% to 50% increased Ne estimates between 82% and 120%, while increasing the minor allele frequency (MAF) threshold from 0.01 to 0.1 decreased estimates between 71% and 75%. Considering these effects is important when interpreting RADseq data‐derived estimates of effective population size in empirical studies. 相似文献
106.
Charrier G Bonhomme M Lacointe A Améglio T 《International journal of biometeorology》2011,55(6):763-774
As observed for most stresses, tree frost resistance can be split into two main processes: avoidance and tolerance. Avoidance
of freezing is achieved by introducing species only in the climatic context in which the probability of freezing events is
very low for the sensitive stages of buds or stems; i.e., when good synchronism exists between the annual cycle and the critical
climatic periods. Buds become able to grow only after chilling requirements have been satisfied (endodormancy released) during
winter; they subsequently break after heat requirements have been completed (end of ecodormancy) in early spring. Actually,
this period is often subject to more or less severe freezing events. Trees are also able to adjust their freezing tolerance
by increasing their capacity of extracellular freezing and decreasing the possibility of intracellular freezing through the
process of frost acclimation. Both freezing resistance processes (avoidance and tolerance) are environmentally driven (by
photoperiod and temperature), but there are also genotypic effects among species or cultivars. Here, we evaluated the degree
to which differences in dormancy release and frost acclimation were related to environmental and genetic influences by comparing
trees growing in common garden conditions. This investigation was carried out for two winters in lowland and mountain locations
on different walnut genotypes differing significantly for budburst dates. Chilling requirement for endodormancy release and
heat requirement during ecodormancy were evaluated in all situations. In addition, frost acclimation was assessed by the electrolyte
leakage method on stems from the same trees before leaf fall through budburst. No significant differences were observed in
chilling requirements among genotypes. Moreover, frost acclimation dynamics were similar between genotypes or locations when
expressed depending on chilling units accumulated since 15 September as a time basis instead of Julian day. The only exception
was for maximal frost hardiness observed during winter with the timber-oriented being significantly more resistant than fruit-oriented
genotypes. Heat requirement was significantly different among genotypes. Thus, growth was significantly faster in fruit-oriented
than in wood-oriented genotypes. Furthermore, among wood-oriented genotypes, differences in growth rate were observed only
at cold temperatures. Frost acclimation changes differed significantly between fruit- and wood- walnuts from January through
budburst. In conclusion, from September through January, the acclimation dynamic was driven mainly by environmental factors
whereas from January through budburst a significant genotype effect was identified in both frost tolerance and avoidance processes. 相似文献
107.
Two complementary segregating plant populations of Coffea canephora were produced from the same clone. One population (DH) comprised 92 doubled haploids derived from female gametes, while the other population (TC) was a test cross consisting of 44 individuals derived from male gametes. Based on the DH population, a genetic linkage map comprising 160 loci was constructed. Eleven linkage groups that putatively correspond to the 11 gametic chromosomes of C. canephora were identified. The mapped loci included more than 40 specific sequence-tagged site markers, either single-copy RFLP probes or microsatellites, that could serve as standard landmarks in coffee-genome analyses. Furthermore, comparisons for segregation distortion and recombination frequency between the two populations were performed. Although segregation distortions were observed in both populations, the frequency of loci exhibiting a very pronounced degree of distortion was especially high in the DH population. This observation is consistent with the hypothesis of strong zygotic selection among the DH population. The recombination frequencies in both populations were found to be almost indistinguishable. These results offer evidence in favour of the lack of significant sex differences in recombination in C. canephora. 相似文献
108.
Bernard Billoud Zofia Nehr Aude Le Bail Bénédicte Charrier 《Nucleic acids research》2014,42(1):417-429
We used an in silico approach to predict microRNAs (miRNAs) genome-wide in the brown alga Ectocarpus siliculosus. As brown algae are phylogenetically distant from both animals and land plants, our approach relied on features shared by all known organisms, excluding sequence conservation, genome localization and pattern of base-pairing with the target. We predicted between 500 and 1500 miRNAs candidates, depending on the values of the energetic parameters used to filter the potential precursors. Using quantitative polymerase chain reaction assays, we confirmed the existence of 22 miRNAs among 72 candidates tested, and of 8 predicted precursors. In addition, we compared the expression of miRNAs and their precursors in two life cycle states (sporophyte, gametophyte) and under salt stress. Several miRNA precursors, Argonaute and DICER messenger RNAs were differentially expressed in these conditions. Finally, we analyzed the gene organization and the target functions of the predicted candidates. This showed that E. siliculosus miRNA genes are, like plant miRNA genes, rarely clustered and, like animal miRNA genes, often located in introns. Among the predicted targets, several widely conserved functional domains are significantly overrepresented, like kinesin, nucleotide-binding/APAF-1, R proteins and CED-4 (NB-ARC) and tetratricopeptide repeats. The combination of computational and experimental approaches thus emphasizes the originality of molecular and cellular processes in brown algae. 相似文献
109.
Holland P Willis C Kanaly S Glaccum M Warren A Charrier K Murison J Derry J Virca G Bird T Peschon J 《Current biology : CB》2002,12(16):1424-1428
The epidermis is a stratified, continually renewing epithelium dependent on a balance among cell proliferation, differentiation, and death for homeostasis. In normal epidermis, a mitotically active basal layer gives rise to terminally differentiating keratinocytes that migrate outward and are ultimately sloughed from the skin surface as enucleated squames. Although many proteins are known to function in maintaining epidermal homeostasis, the molecular coordination of these events is poorly understood. RIP4 is a novel RIP (receptor-interacting protein) family kinase with ankyrin repeats cloned from a keratinocyte cDNA library. RIP4 deficiency in mice results in perinatal lethality associated with abnormal epidermal differentiation. The phenotype of RIP4(-/-) mice in part resembles that of mice lacking IKKalpha, a component of a complex that regulates NF-kappaB. Despite the similar keratinocyte defects in RIP4- and IKKalpha-deficient mice, these kinases function in distinct pathways. RIP4 functions cell autonomously within the keratinocyte lineage. Unlike IKKalpha, RIP4-deficient skin fails to fully differentiate when grafted onto a normal host. Instead, abnormal hair follicle development and epidermal dysplasia, indicative of progression into a more pathologic state, are observed. Thus, RIP4 is a critical component of a novel pathway that controls keratinocyte differentiation. 相似文献
110.
Y Deng J Zhao D Sakurai KM Kaufman JC Edberg RP Kimberly DL Kamen GS Gilkeson CO Jacob RH Scofield CD Langefeld JA Kelly ME Alarcón-Riquelme BIOLUPUS GENLES Networks JB Harley TJ Vyse BI Freedman PM Gaffney KM Sivils JA James TB Niewold RM Cantor W Chen BH Hahn EE Brown PROFILE BP Tsao 《Arthritis research & therapy》2012,14(Z3):A5