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991.
Beth A Beidleman Stephen R Muza Charles S Fulco Allen Cymerman Dan T Ditzler Dean Stulz Janet E Staab Scott R Robinson Gary S Skrinar Steven F Lewis Michael N Sawka 《Journal of applied physiology》2003,95(5):1824-1832
Chronic altitude residence improves muscular performance at altitude, but the effect of intermittent altitude exposures (IAE) on muscular performance at altitude has not been defined. The purpose of this study was to determine the effects of 3 wk of IAE, in combination with rest and cycle training, on muscular performance at altitude. Six lowlanders (23 +/- 2 yr, 77 +/- 6 kg; means +/- SE) completed a cycle time trial and adductor pollicis endurance test at sea level and during a 30-h acute exposure to 4,300 m altitude equivalent (barometric pressure = 446 mmHg) once before (pre-IAE) and once after (post-IAE) a 3-wk period of IAE (4 h/day, 5 days/wk, 4,300 m). During each IAE, three subjects cycled for 45-60 min/day at 60%-70% of maximal O2 uptake and three subjects rested. Cycle training during each IAE did not appear to affect muscular performance at altitude. Thus data from all six subjects were combined. Three weeks of IAE resulted in 1) a 21 +/- 6% improvement (P < 0.05) in cycle time-trial performance (min) from pre-IAE (32.8 +/- 3.7) to post-IAE (24.8 +/- 1.2), 2) a 63 +/- 26% improvement (P < 0.05) in adductor pollicis endurance (min) from pre-IAE (9.2 +/- 2.8) to post-IAE (14.8 +/- 4.2), and 3) a 10 +/- 4% increase (P < 0.05) in resting arterial O2 saturation (%) from pre-IAE (82 +/- 2) to post-IAE (90 +/- 1). These improvements in muscular performance after IAE correlated strongly with increases in resting arterial O2 saturation and were comparable to those reported previously after chronic altitude residence. IAE may therefore be used as an alternative to chronic altitude residence to facilitate improvements in muscular performance in athletes, soldiers, mountaineers, shift workers, and others that are deployed to altitude. 相似文献
992.
This study sought to investigate land‐snail diversity, abundance and distribution in Arabuko Sokoke forest. Sampling was done using standard timed direct search and litter sample methods. In total, 25 species and 1263 specimens were recorded. The 25 species rank Arabuko Sokoke forest as the second richest coastal forest in land snails in East Africa. The 25 species, however, are not uniformly distributed across the forest. The majority of the snails were localized with low levels of abundance. Eight species were restricted to one forest type, whereas those shared among forest types tended to concentrate in one forest type. Only five species, Gulella radius, Gulella foveolata, Gonaxis kibweziensis, Pseudoglessula biovini and Opeas gracilis, were widespread. The highest snail diversity was recorded in the mixed forest, implying that special conservation of this habitat is necessary for retention of the broadest molluscan diversity. The observation that some species rare in the mixed forest were recorded in excess from Brachystegia and Cynometra forest, further suggests that conservation of the entire A. Sokoke forest is critical for efficient molluscan conservation. Species recorded in low levels of abundance are probably declining and investigation on the snails' ecology to understand factors that influence the snails' diversity is recommended. 相似文献
993.
994.
Charles J. Goodnight 《Evolution; international journal of organic evolution》1988,42(3):441-454
Models of founder events have focused on the reduction in the genetic variation following a founder event. However, recent work (Bryant et al., 1986; Goodnight, 1987) suggests that when there is epistatic genetic variance in a population, the total genetic variance within demes may actually increase following a founder event. Since the additive genetic variance is a statistical property of a population and can change with the level of inbreeding, some of the epistatic genetic variance may be converted to additive genetic variance during a founder event. The model presented here demonstrates that some of the additive-by-additive epistatic genetic variance is converted to additive genetic variance following a founder event. Furthermore, the amount of epistasis converted to additive genetic variance is a function of the recombination rate and the propagule size. For a single founder event of two individuals, as much as 75% of the epistatic variance in the ancestral population may become additive genetic variance following the founder event. For founder events involving two individuals with free recombination, the relative contribution of epistasis to the additive genetic variance following a founder event is equal to its proportion of the total genetic variance prior to the founder event. Traits closely related to fitness are expected to have relatively little additive genetic variance but may have substantial nonadditive genetic variance. Founder events may be important in the evolution of fitness traits, not because they lead to a reduction in the genetic variance, but rather because they lead to an increase in the additive genetic variance. 相似文献
995.
Gametophytic competition among pollen grains has been proposed as an important mechanism of sexual selection in plants. The purpose of this paper is to examine the contribution of pollen source on in vivo pollen tube growth in Chamaecrista fasciculata. We addressed two questions: 1) Is pollen tube growth affected by the genetic relatedness between the pollen source and the pollen recipient? 2) Is there significant phenotypic variation among pollen donors for pollen tube growth? We compared pollen tube growth by measuring the number of pollen tubes which germinated, reached quarter length of style, and reached the ovary resulting from self- and outcross-pollinations. The outcross pollinations included three interplant distance classes: near (within genetic neighborhood, ca. 1 m), far (between far neighborhoods and within subpopulation, ca. 20 m), and distant (between neighborhoods and adjacent subpopulations, ca. 50–100 m). Our results show that pollen tube growth was not affected by genetic relatedness, by differences between self and outcross, nor by differences due to phenotypic variation among pollen donors. In contrast, maternal environment had a strong impact on pollen tube growth. These results suggest a lack of gametophytic competition and indicate little opportunity for sexual selection on pollen tube growth in C. fasciculata. 相似文献
996.
Baroreceptor control of the cutaneous active vasodilator system 总被引:2,自引:0,他引:2
Crandall C. G.; Johnson J. M.; Kosiba W. A.; Kellogg D. L. Jr. 《Journal of applied physiology》1996,81(5):2192-2198
Crandall, C. G., J. M. Johnson, W. A. Kosiba, and D. L. Kellogg, Jr. Baroreceptor control of the cutaneous activevasodilator system. J. Appl. Physiol.81(5): 2192-2198, 1996.We sought to identify whether reductionsin cutaneous active vasodilation during simulated orthostasis could beassigned solely to cardiopulmonary or to carotid baroreflexes byunloading cardiopulmonary baroreceptors with low levels of lower bodynegative pressure (LBNP) or unloading carotid baroreceptors withexternal pressure applied over the carotid sinus area [carotidpressure (CP)]. Skin blood flow was measured at a site at whichadrenergic function was blocked via bretylium tosylate iontophoresisand at an unblocked site. During LBNP of 5 and10 mmHg in hyperthermia, neither heart rate (HR) nor cutaneousvascular conductance (CVC) at either site changed (P > 0.05 for both), whereas forearmvascular conductance (FVC) was reduced (5 mmHg: from 21.6 ± 4.8 to 19.8 ± 4.1 FVC units, P = 0.05; 10 mmHg: from 22.3 ± 4.0 to 19.3 ± 3.7 FVC units,P = 0.002). LBNP of 30 mmHg inhyperthermia reduced CVC at both sites (untreated: from 51.9 ± 5.7 to 43.2 ± 5.1% maximum, P = 0.02;bretylium tosylate: from 60.9 ± 5.4 to 53.2 ± 4.4% maximum, P = 0.02), reduced FVC (from 23.2 ± 3.6 to 18.1 ± 3.3 FVC units; P = 0.002), and increased HR (from 83 ± 4 to 101 ± 3 beats/min; P = 0.003). Pulsatile CP (45 mmHg) did not affect FVC or CVC during normothermia or hyperthermia (P > 0.05). However, HR and mean arterial pressure were elevated during CPin both thermal conditions (both P < 0.05). These results suggest that neither selective low levels ofcardiopulmonary baroreceptor unloading nor selective carotidbaroreceptor unloading can account for the inhibition of cutaneousactive vasodilator activity seen with simulated orthostasis. 相似文献
997.
Dreshaj Ismail A.; Haxhiu Musa A.; Potter Charles F.; Agani Faton H.; Martin Richard J. 《Journal of applied physiology》1996,81(4):1785-1791
Dreshaj, Ismail A., Musa A. Haxhiu, Charles F. Potter, FatonH. Agani, and Richard J. Martin. Maturational changes in responsesof tissue and airway resistance to histamine. J. Appl.Physiol. 81(4): 1785-1791, 1996.We determinedhow postnatal maturation affects the relative contributions of airwaysand lung parenchyma to pulmonary resistance(RL) and whether there are developmental differences in their respective responses to constrictive agents. We studied open-chest ventilated anesthetized piglets of threeages: 2-4 days, 2-3 wk, and 10 wk.RL was partitioned into tissue(Rti) and airway (Raw) resistance by means of alveolar capsules underbaseline conditions and after intravenous histamine. Postnatalmaturation was associated with a progressive decline inRL, Rti, and Raw and with anincrease in the contribution of Rti toRL from 38 ± 8% at 2-4days to 72 ± 2% at both 2-3 and 10 wk. Histamine causedRL to increase at all ages. Whenpartitioned into Rti and Raw, the percent increase in Rti significantlyexceeded that of Raw at both 2-4 days and 2-3 wk. Incontrast, the percent increase in Raw significantly exceeded that ofRti at 10 wk. Administration of atropine before histamine in pigletsaged 10 wk reduced the response of Rti and Raw to histamine.Histamine-induced responses ofRL were blocked by priorH1-receptor blockade withpyrilamine (2 mg/kg). These results indicate that1) the contribution of Rti and Rawto RL changes during maturationand that 2) contractile responses toexogenous histamine are manifest predominantly in most distal airwaysand lung parenchyma during early postnatal life; with advancingmaturation there is greater contribution of airways to the increase inRL induced by histamine. 相似文献
998.
999.
Inhibition of Salmonella typhimurium by the products of tartrate metabolism by a Veillonella species
The inhibition of the growth of Salmonella typhimurium by a Veillonella species grown on media supplemented with tartrate was examined. Growth of Salmonella typhimurium was not inhibited by the concentrations of products metabolized by Veillonella cultures on media supplemented with 0 or 50 mmol 1-1 of tartrate, but was inhibited on media supplemented with 100 or 150 mmol 1-1 of tartrate. Inhibition of Salm. typhimurium was correlated with the increased production of acetate and propionate from tartrate by the Veillonella species. 相似文献
1000.
D. H. Brown Jr I. V. Slobodkin C. A. Kumamoto 《Molecular genetics and genomics : MGG》1996,251(1):75-80
To allow the regulated expression of cloned genes inCandida albicans, a plasmid was constructed using the inducible promoter of theC. albicans MAL2 gene. To demonstrate that theMAL2 promoter could regulate cloned genes placed under its control, a fusion construct was made with the coding sequence of theC. albicans URA3 gene. This plasmid was introduced into a Ura? strain ofC. albicans using the process of restriction enzyme-mediated integration (REMI). This procedure involves the transformation of theBamHI-linearized plasmid in the presence ofBamHI enzyme. The majority of transformants generated contained insertions of the plasmid at chromosomalBamHI sites. All transformants examined were inducible forURA3 expression, which was determined by growth analysis and by measuring the level ofURA3 gene product activity. The Ura+ phenotype of the transformants was stable during growth under nonselective conditions. This system offers the advantages of stable transformation, easy recovery of integrated DNA, and inducible expression of genes inC. albicans. 相似文献