首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   150篇
  免费   6篇
  2021年   3篇
  2020年   1篇
  2019年   1篇
  2018年   2篇
  2017年   2篇
  2016年   1篇
  2015年   4篇
  2014年   3篇
  2013年   12篇
  2012年   13篇
  2011年   10篇
  2010年   13篇
  2009年   6篇
  2008年   10篇
  2007年   3篇
  2006年   7篇
  2005年   7篇
  2004年   4篇
  2003年   2篇
  2002年   1篇
  2001年   3篇
  2000年   1篇
  1999年   1篇
  1998年   4篇
  1997年   2篇
  1993年   1篇
  1992年   2篇
  1987年   4篇
  1986年   4篇
  1985年   2篇
  1984年   2篇
  1983年   1篇
  1982年   1篇
  1981年   1篇
  1980年   2篇
  1979年   3篇
  1978年   3篇
  1977年   4篇
  1975年   1篇
  1973年   2篇
  1972年   1篇
  1971年   1篇
  1970年   1篇
  1962年   1篇
  1961年   1篇
  1955年   1篇
  1948年   1篇
排序方式: 共有156条查询结果,搜索用时 296 毫秒
41.
42.
43.
44.

Background  

The neighbor-joining method by Saitou and Nei is a widely used method for constructing phylogenetic trees. The formulation of the method gives rise to a canonical Θ(n 3) algorithm upon which all existing implementations are based.  相似文献   
45.
A consistent, if not invariant, feature of cancer cells is the acquired ability to evade apoptosis. The pioneering work of Dr. Stan Korsmeyer was invaluable in characterizing the molecular foundations of cell death signaling mechanisms during normal development and during multistep carcinogenesis. This foundation now forms the basis for the rational design of therapeutic strategies to selectively activate cell death in cancer cell populations. These strategies are currently being evaluated in an increasing number of clinical trials targeting diverse tumor types.  相似文献   
46.
47.
264W94 was designed to inhibit the ileal bile acid transporter (IBAT). Evaluated in vitro, 264W94 dose-dependently inhibited sodium-dependent uptake of 10 micro M [(3)H]taurocholic acid (TC) by rat and monkey brush border membrane vesicles with IC(50)s of 0.24 micro M and 0.41 micro M, and had a competitive profile with K(i) of 0.2 micro M against TC in Chinese hamster ovary cells expressing human IBAT. In distal ileum in situ, 1-10 micro M of 264W94 rapidly decreased uptake of 3mM TC by 24-39%, with corresponding decreases in biliary recovery. In rats and mice in vivo, oral 264W94 decreased absorption of TC analog, 23,25-(75)Se-homocholic acid taurine ((75)SeHCAT; quantitated in feces), with ED(30) of 0.02 mg/kg bid. (75)SeHCAT traced through the GI-tract revealed that peak (97%) inhibition of (75)SeHCAT absorption by the distal quarter of small intestine occurred at 4 h after single dose of 264W94 (0.1 mg/kg). Inhibition of IBAT by 264W94 in rats was associated with compensatory, same-day, 4-fold induction of hepatic cholesterol 7alpha-hydroxylase (CYP7A1) activity, exhibiting normal diurnal fluctuation for 3 days of dosing. In diet induced hypercholesterolemic rats, 264W94 (0.03-1.0 mg/kg bid) dose-dependently reduced serum LDL+VLDL cholesterol up to 61%. In conclusion, 264W94 is a potent new cholesterol lowering agent that acts through inhibition of IBAT and exhibits activity in a human model.  相似文献   
48.
cis-Acting Sequences Required for Simian Foamy Virus Type 1 Vectors   总被引:1,自引:0,他引:1       下载免费PDF全文
We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of a deletion mutation in the leader region between the 5′ long terminal repeat and the start of the gag gene at position 1659 to 1694 completely abrogated gene transfer by the SFV-1 vector. Deletion of 39 nucleotides from position 1692 to 1731 in the leader region resulted in a significant reduction in the transducing-particle titer. Furthermore, we have identified a second cis-acting element located at the 3′ end of the pol gene between position 6486 and 6975 to be critical for SFV-1 vector transduction. These results identify the two important cis-acting elements required for SFV-1 vector construction, and the finding of a cis-acting element in the pol gene is unique among retroviruses.  相似文献   
49.
Synthesis of (+)-11-hydroxyhexadecanoic acid-O-(4-O-α-L-rhamnopyranosyl)-α-L- rhamnopyranoside showed that muricatin B from Ipomoea muricata does not contain the proposed β- but an α-linked dirhamnoside moiety. This is the first reported synthesis of a naturally occurring O-glycoside of a long chain hydroxy-fatty acid.  相似文献   
50.
The use of drug-antibody conjugates affords a method for the targeted delivery of anticancer drugs specifically to cancer cells. Monoclonal antibodies alone usually do not possess high therapeutic efficacy, however, they are capable of targeting tumor markers selectively. We have prepared taxoids with significantly higher cytotoxicity than paclitaxel and docetaxel. These taxoids now meet the high potency required for use in a targeted-delivery approach using monoclonal antibodies. The synthesis and biological evaluation of these taxoids are reported.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号