全文获取类型
收费全文 | 305篇 |
免费 | 30篇 |
出版年
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 12篇 |
2013年 | 11篇 |
2012年 | 13篇 |
2011年 | 11篇 |
2010年 | 8篇 |
2009年 | 8篇 |
2008年 | 16篇 |
2007年 | 11篇 |
2006年 | 8篇 |
2005年 | 16篇 |
2004年 | 14篇 |
2003年 | 6篇 |
2002年 | 12篇 |
2001年 | 15篇 |
2000年 | 7篇 |
1999年 | 9篇 |
1998年 | 10篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 7篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 9篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1986年 | 3篇 |
1984年 | 3篇 |
1981年 | 2篇 |
1977年 | 6篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1966年 | 2篇 |
1963年 | 4篇 |
1918年 | 2篇 |
1912年 | 1篇 |
1911年 | 2篇 |
排序方式: 共有335条查询结果,搜索用时 125 毫秒
321.
322.
323.
Further characterization and species specificity of leukocytic endogenous mediator (LEM) 总被引:3,自引:0,他引:3
324.
Jeffrey P Simpson Cole Wunderlich Xu Li Elizabeth Svedin Brian Dilkes Clint Chapple 《The Plant cell》2021,33(3):492
The optimal extraction of information from untargeted metabolomics analyses is a continuing challenge. Here, we describe an approach that combines stable isotope labeling, liquid chromatography– mass spectrometry (LC–MS), and a computational pipeline to automatically identify metabolites produced from a selected metabolic precursor. We identified the subset of the soluble metabolome generated from phenylalanine (Phe) in Arabidopsis thaliana, which we refer to as the Phe-derived metabolome (FDM) In addition to identifying Phe-derived metabolites present in a single wild-type reference accession, the FDM was established in nine enzymatic and regulatory mutants in the phenylpropanoid pathway. To identify genes associated with variation in Phe-derived metabolites in Arabidopsis, MS features collected by untargeted metabolite profiling of an Arabidopsis diversity panel were retrospectively annotated to the FDM and natural genetic variants responsible for differences in accumulation of FDM features were identified by genome-wide association. Large differences in Phe-derived metabolite accumulation and presence/absence variation of abundant metabolites were observed in the nine mutants as well as between accessions from the diversity panel. Many Phe-derived metabolites that accumulated in mutants also accumulated in non-Col-0 accessions and was associated to genes with known or suspected functions in the phenylpropanoid pathway as well as genes with no known functions. Overall, we show that cataloguing a biochemical pathway’s products through isotopic labeling across genetic variants can substantially contribute to the identification of metabolites and genes associated with their biosynthesis.An isotopic labeling and LC–MS pipeline to identify metabolites produced from Phe and its integration with genome-wide association identifies genes associated with the phenylpropanoid pathway. 相似文献
325.
326.
Sensilla on the antennae of eight species of noctuid moth—Agrotis ipsilon, Graphania morosa, G. mutans, G. ustistriga, Leucania unica, Persectania atristiga, P. aversa, and Pseudaletia separata—were examined with the scanning electron microscope. At least six types of sensillum described by other authors were distinguishable within this material. However, separation of all the sensilla into distinct morphological types proved difficult, and a continuous spectrum of types is inferred and discussed. 相似文献
327.
328.
Anna Block Joshua R. Widhalm Abdelhak Fatihi Rebecca E. Cahoon Yashitola Wamboldt Christian Elowsky Sally A. Mackenzie Edgar B. Cahoon Clint Chapple Natalia Dudareva Gilles J. Basset 《The Plant cell》2014,26(5):1938-1948
It is not known how plants make the benzenoid ring of ubiquinone, a vital respiratory cofactor. Here, we demonstrate that Arabidopsis thaliana uses for that purpose two separate biosynthetic branches stemming from phenylalanine and tyrosine. Gene network modeling and characterization of T-DNA mutants indicated that acyl-activating enzyme encoded by At4g19010 contributes to the biosynthesis of ubiquinone specifically from phenylalanine. CoA ligase assays verified that At4g19010 prefers para-coumarate, ferulate, and caffeate as substrates. Feeding experiments demonstrated that the at4g19010 knockout cannot use para-coumarate for ubiquinone biosynthesis and that the supply of 4-hydroxybenzoate, the side-chain shortened version of para-coumarate, can bypass this blockage. Furthermore, a trans-cinnamate 4-hydroxylase mutant, which is impaired in the conversion of trans-cinnamate into para-coumarate, displayed similar defects in ubiquinone biosynthesis to that of the at4g19010 knockout. Green fluorescent protein fusion experiments demonstrated that At4g19010 occurs in peroxisomes, resulting in an elaborate biosynthetic architecture where phenylpropanoid intermediates have to be transported from the cytosol to peroxisomes and then to mitochondria where ubiquinone is assembled. Collectively, these results demonstrate that At4g19010 activates the propyl side chain of para-coumarate for its subsequent β-oxidative shortening. Evidence is shown that the peroxisomal ABCD transporter (PXA1) plays a critical role in this branch. 相似文献
329.
330.