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71.
A. J. Soitamo G. Zhou A. K. Clarke G. Öquist E-M. Aro P. Gustafsson 《Plant molecular biology》1994,26(2):709-721
The unicellular cyanobacterium Synechococcus sp. PCC 7942 has three psbA genes encoding two different forms of the photosystem II reaction centre protein D1 (D1:1 and D1:2). The level of expression of these psbA genes and the synthesis of D1:1 and D1:2 are strongly regulated under varying light conditions. In order to better understand the regulatory mechanisms underlying these processes, we have constructed a strain of Synechococcus sp. PCC 7942 capable of over-producing psbA mRNA and D1 protein. In this study, we describe the over-expression of D1:1 using a tac-hybrid promoter in front of the psbAI gene in combination with lacI
Q
repressor system. Over-production of D1:1 was induced by growing cells for 12 h at 50 mol photons m-2 s-1 in the presence of 40 or 80 g/ml IPTG. The amount of psbAI mRNA and that of D1:1 protein in cells grown with IPTG was three times and two times higher, respectively. A higher concentration of IPTG (i.e., 150 g/ml) did not further increase the production of the psbAI message or D1:1. The over-production of D1:1 caused a decrease in the level of D1:2 synthesised, resulting in most PSII reaction centres containing D1:1. However, the over-production of D1:1 had no effect on the pigment composition (chlorophyll a or phycocyanin/number of cells) or the light-saturated rate of photosynthesis. This and the fact that the total amounts of D1 and D2 proteins were not affected by IPTG suggest that the number of PSII centres within the membranes remained unchanged. From these results, we conclude that expression of psbAI can be regulated by using the tac promoter and lacI
Q
system. However, the accumulation of D1:1 protein into the membrane is regulated by the number of PSII centres. 相似文献
72.
The Escherichia coli arginine repressor (ArgR) is an l -arginine-dependent DNA-binding protein that controls expression of the arginine biosynthetic genes and is required as an accessory protein in Xer site-specific recombination at cer and related recombination sites in plasmids. Site-directed mutagenesis was used to isolate two mutants of E. coli ArgR that were defective in arginine binding. Results from in vivo and in vitro experiments demonstrate that these mutants still act as repressors and bind their specific DNA sequences in an arginine-independent manner. Both mutants support Xer site-specific recombination at cer. One of the mutant proteins was purified and shown to bind to its DNA target sequences in vitro with different affinity and as a different molecular species to wild-type ArgR. 相似文献
73.
Summary A novel method of lactic acid fermentation byLactobacillus casei immobilized in Ca—alginate gels is described, in which an ion—exchange resin packed column is attached to a fermentor for
separation of lactic acid from fermentative broth. The technique successfully alleviated the restriction imposed by lactic
acid on bacterial growth and product formation. As compared to the conventional batch fermentation, the new fermentation technique
enhanced the lactic acid productivity and sugar conversion rate from 0.328g/L·h and 88. 2% to 0.482g/L·h and 98.6%, respectively. 相似文献
74.
75.
麦蛾的求偶行为与区分等级的方法 总被引:5,自引:2,他引:3
求偶行为是麦蛾的本能,主要由自身生理状态决定,雌蛾全是自发行为,雄蛾多是对异性信息刺激的反应。两性求偶行为都有固定发生程序,有明显的阶段性。本文按其阶段程序特点把两性求偶行为各分为3等9级。非求偶行为概作0等0级。等级数值大小反映了性兴奋强度,统计分析级别数值,就能确定个体或蛾群求偶行为的动态。 相似文献
76.
黑龙江依兰早第三纪植物群的古气候分析 总被引:5,自引:0,他引:5
对黑龙江依兰煤矿煤层间的大量植物叶痕化石研究表明:依兰植物群有蕨类植物2 种,裸子植物10 种,被子植物58 种,分属34 科46属。植物群可分为两个植物组合:一个是下煤层上顶板的矿页岩层化石的组合,称A 段组合,时代为早始新世。植物种类丰富,含有较多常绿阔叶成分,属北亚热带的常绿阔叶和落叶阔叶、针阔叶混生林。通过植物叶相特征分析,其全缘叶比例为38% 。用气候诺模图得出其古气候为年均温13.2℃,年温差20℃;另一个植物组合是煤系地层之上,即上煤层顶部的油页岩层中的B段化石组合,时代为早渐新世。植物以落叶成分为主,属暖温带落叶阔叶林和针阔叶混生林。全缘叶比例为30% 。古气候年均温为11℃,年温差25℃。表明植物区系组成完全不同,显示出气候随时代发生了演变,而使区系逐渐发展到今日的寒温带气候和植被 相似文献
77.
Summary A method was developed for the introduction of plasmids into Clostridium botulinum by electroporation. A 4.4 kb plasmid vector, pGK12, which contains genes for resistance to erythromycin (Emr) and chloramphenicol (Cmr) was electroporated into C. botulinum type A (Hall A). The highest transformation efficiency was obtained using midlog phase cells, 10% PEG 8000 as the electroporation solution, and 2.5 kV field strength. The transformation efficiency was highest (103 transformants/g of DNA) when 1 g of plasmid DNA and 4 × 108 CFU/ml of recipient cells were used. Plasmid DNA recovered from the transformants was indistinguishable from that introduced on the basis of restriction enzyme digestion and agarose gel electrophoresis. 相似文献
78.
79.