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61.
本文报道在我国广西隆林壮族中发现一个罕見的HbQ复合α,β地中海贫血家系。先证者女,18岁,贫血面容,肝脾肿大。化学结构分析确证本Hb变异体为HbQ Thailand[α74(EF3)Asp→His]。血红蛋白组成以及α和β珠蛋白基因分析结果表明,先证者的珠蛋白基因型为-α~Q/-α~T复合β°/β°(IVSI-1G→T/Codon17A→T);先证者父的基因型为-‘α~Q/-复合β~O/β~A(IVSI-1G→T/β~A);先证母的基因型为-α~T/αα复合β~O/β~A(Codon17A→T/β~A)。 相似文献
62.
Using cytochemical method,microspectrophotometry and image analysis,effects of va-soactive intestinal peptide(VIP)on activities of succinic dehydrogenase(SDH)and alkalinephosphatase(ALP)in rat hepatoma cells were studied in vitro.The results showed that thehepatoma cell expressed potent positive reactions of SDH and ALP,the positive positionswere located at the cell membranes and/or cytoplasm.Having been treated with VIP,ALPdecreased obviously in activity(P<0. 01,compared with hepatoma cells untreated by VIP).The sites of ALP activty were chiefly located at the cell membranes,particularly at the cell-cell contacts.Cultured rat hepatoma cells had intensive SDH activity in their cytoplasm.Compared with untreated eclls,there was no marked difference in the intensity of SDH activ-ity in VIP-treated hepatoma cells(P>0.05). 相似文献
63.
H. T. Huang 《Applied microbiology》1961,9(5):419-424
Two auxotrophic mutants of Escherichia coli have been shown to accumulate significant amounts of l-threonine in the culture medium. One mutant, 13071, is deficient in α,ε-diaminopimelic acid (DAP), and the other, 13070, is deficient in both DAP and methionine. 相似文献
64.
The gene (alyVI) encoding an alginate lyase of marine bacterium Vibrio sp. QY101, which was isolated from a decaying thallus of Laminaria, was cloned using a strategy of combined degenerate PCR and long range-inverse PCR (LR-IPCR), then sequenced and expressed in Escherichia coli. Gene alyVI was composed of a 1014 bp open reading frame (ORF) encoding 338 amino acid residues. The calculated molecular mass of alyVI product is 38.4 kDa, but a signal peptide is cleaved off, leaving a mature protein of 34 kDa. AlyVI was purified from culture supernatants to electrophoretic homogeneity using affinity chromatography. AlyVI was most active at pH 7.5 and 40 degrees C in the presence of 1 mM ZnCl2. A nine-amino-acid consensus region (YXRESLREM), which was only found in polyguluronate lyases, was also observed in the amino-terminal region of AlyVI. However, AlyVI could degrade both M block and G block. These results indicate that a novel alginate lyase-encoding gene has been cloned. 相似文献
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67.
国家自然科学基金评审程序剖析──1993年和1994年植物学科面上基金项目分析陈峰,朱大保(中国科学院武汉植物研究所武汉430074)(国家自然科学基金委员会生命科学部北京100083)关键词基金,评审,科研立项,学科ANALYSLSFORJUDGE... 相似文献
68.
本文利用小鼠大脑机械损伤模型及体外培养的胶质细胞,采用同位素渗入法观察了细胞介素及其抗体对胶质细胞增生的影响。结果表明:体外培养时TNF-α在浓度为10~200u/ml培养液时均能促进胶质细胞增生(P<0.05),IL1-β在浓度为5~200u/ml培养液时能促进胶质细胞增生,TNF-α+IL1-β其促进胶质细胞增生作用更强烈,TNF-α抗体能完全阻断TNF-α的促增生作用,部分阻断TNF-α+IL1-β的促增生作用。在体实验时,IL1-β及TNF-α的作用与离体时相似,IL1-β及TNF-α亦能促进胶质细胞增生,二者共同作用时促细胞增生作用更强。以上结果提示,外源性TNF-α及IL1-β能促进中枢神经损伤后胶质细胞增生且具有协同作用。 相似文献
69.
EB病毒胸苷激酶基因的扩增 总被引:1,自引:0,他引:1
EB病毒胸苷激酶基因的扩增陈尚武,陈瑞君,黄迪,朱振宇,马涧泉(中山医科大学生化教研室,广州510089)(中山医科大学肿瘤研究所,广州510060)关键词Epstein-Barr病毒,胸苷激酶,基因,聚合酶链反应鼻咽癌(nasopharyngeal... 相似文献
70.
Blackfoot disease is a peripheral vascular disease resulting in gangrene of the lower extremities. Although extensive epidemiological
study has implicated high arsenic content in artesian well water of the endemic area bears some important connection with
the disease, the etiology of the disease is still not clarified. In this study, attention is paid to chemical speciation of
arsenic in order to find out whether the concentrations of arsenic species in urine of Blackfoot disease patients are different
from those of controls. Experimental results indicate that the total arsenic, inorganic arsenic, monomethylarsonic acid, and
other forms of arsenic in the urine of patients are significantly higher than those of the contols. The possible connection
of those arsenic species with the etiology of the disease is discussed. 相似文献