全文获取类型
收费全文 | 13221篇 |
免费 | 1184篇 |
国内免费 | 1353篇 |
出版年
2024年 | 28篇 |
2023年 | 166篇 |
2022年 | 429篇 |
2021年 | 740篇 |
2020年 | 520篇 |
2019年 | 647篇 |
2018年 | 593篇 |
2017年 | 482篇 |
2016年 | 646篇 |
2015年 | 933篇 |
2014年 | 1085篇 |
2013年 | 1074篇 |
2012年 | 1241篇 |
2011年 | 1097篇 |
2010年 | 727篇 |
2009年 | 611篇 |
2008年 | 654篇 |
2007年 | 528篇 |
2006年 | 476篇 |
2005年 | 424篇 |
2004年 | 388篇 |
2003年 | 316篇 |
2002年 | 292篇 |
2001年 | 187篇 |
2000年 | 158篇 |
1999年 | 161篇 |
1998年 | 79篇 |
1997年 | 98篇 |
1996年 | 81篇 |
1995年 | 76篇 |
1994年 | 62篇 |
1993年 | 62篇 |
1992年 | 105篇 |
1991年 | 85篇 |
1990年 | 72篇 |
1989年 | 64篇 |
1988年 | 36篇 |
1987年 | 55篇 |
1986年 | 43篇 |
1985年 | 39篇 |
1984年 | 25篇 |
1983年 | 23篇 |
1982年 | 15篇 |
1981年 | 11篇 |
1980年 | 18篇 |
1977年 | 11篇 |
1972年 | 11篇 |
1971年 | 9篇 |
1970年 | 9篇 |
1969年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
Zelong Miao Chao Xiong Xiaolong Cao Tisheng Shan Qiao Jin Haobo Jiang 《Insect Science》2023,30(2):338-350
Serine esterases (SEs) are hydrolases that catalyze the conversion of carboxylic esters into acids and alcohols. Lipases and carboxylesterases constitute two major groups of SEs. Although over a hundred of insect genomes are known, systematic identification and classification of SEs are rarely performed, likely due to large size and complex composition of the gene family in each species. Considering their key roles in lipid metabolism and other physiological processes, we have categorized 144 M. sexta SEs and SE homologs (SEHs), 114 of which contain a motif of GXSXG. Multiple sequence alignment and phylogenetic tree analysis have revealed 39 neutral lipases (NLs), 3 neutral lipase homologs (NLHs), 11 acidic lipases (ALs), 3 acidic lipase homologs (ALHs), a lipase-3, a triglyceride lipase, a monoglyceride lipase, a hormone-sensitive lipase, and a GDSL lipase. Eighty-three carboxylesterase genes encode 29 α-esterases (AEs), 12 AEHs (e.g., SEH4-1–3), 20 feruloyl esterases (FEs), 2 FEHs, 2 β-esterases (BEs), 2 integument esterases (IEs), 1 IEH, 4 juvenile hormone esterases, 2 acetylcholinesterases, gliotactin, 6 neuroligins, neurotactin, and an uncharacteristic esterase homolog. In addition to these GXSXG proteins, we have identified 26 phospholipases and 13 thioesterases. Expression profiling of these genes in specific tissues and stages has provided insights into their functions including digestion, detoxification, hormone processing, neurotransmission, reproduction, and developmental regulation. In summary, we have established a framework of information on SEs and related proteins in M. sexta to stimulate their research in the model species and comparative investigations in agricultural pests or disease vectors. 相似文献
112.
113.
114.
115.
生物成矿作用与生物矿化作用 总被引:6,自引:0,他引:6
列举55种生物矿物,其中包括氧化物,含氧盐,水化物,羟基化物,钙化合物,指出它们通常经历非晶质、过渡、成熟和石化等4个相阶段。根据生物矿物体的元素丰度顺序与岩石圈和生物圈对比,将生矿元素分为6类。提出生矿元素保留于生物体内8种形式,即可溶无机化合物、有机络合物、生矿粒、生矿簇、骨针、骨片、骨壳和骨骼,并以磷为例阐明了生矿元素的富集过程。按照生物作用型式,建议划分生物矿床为堆积、粘结、吸附和储集4种成因类型,分别讨论它们成矿的生物、矿物和矿床,分析形成的环境、过程和阶段,并介绍中国某些著名生物矿床实例。最后总结了生物矿床的地史演化趋向:金属硫化物矿床→条带状硅铁(锰)粘结矿床→叠层状白云岩粘结矿床→磷酸盐和碳酸锰粘结矿床→铅锌和油气储集矿床→煤和油气。 相似文献
116.
117.
The plasma membrane of most cells is drawn tightly over the cytoskeleton of the cell, resulting in a significant tension being developed in the membrane. The tension in the membrane can be calculated from the force required to separate it from the cytoskeleton; and the force itself can be measured rapidly by using laser tweezers. Recent observations indicate that decreasing membrane tension stimulates endocytosis and increasing tension stimulates secretion. Thus, membrane tension provides a simple physical mechanism to control the area of the plasma membrane. Here, we speculate that tension is a global parameter that the cell uses to control physically plasma membrane dynamics, cell shape and cell motility. 相似文献
118.
动物细胞在鼓泡式生物反应器中的死亡速率 总被引:1,自引:1,他引:0
通过实验测定,证明生物反应器中细胞死亡速率与气体鼓泡速率成正比而与反应器体积成反比。实验发现气泡大小对细胞死亡速率具有两种作用,一种作用在于影响气泡表面积生成速率;另一种作用则在于影响细胞在气泡表面的吸附程度,其最佳直径为5mm左右。血清和Pluronic F68能显著降低细胞死亡速率,当Pluronic F68浓度达到0.1%时,kd趋于零。所有这些实验结果均与前文提出的生物反应器设计模型具有很好的一致性。 相似文献
119.
C4 Photosynthesis (The Effects of Leaf Development on the CO2-Concentrating Mechanism and Photorespiration in Maize) 总被引:1,自引:1,他引:0 下载免费PDF全文
The effect of O2 on photosynthesis was determined in maize (Zea mays) leaves at different developmental stages. The optimum level of O2 for maximum photosynthetic rates was lower in young and senescing tissues (2-5 kPa) than in mature tissue (9 kPa). Inhibition of photosynthesis by suboptimal levels of O2 may be due to a requirement for functional mitochondria or to cyclic/pseudocyclic photophosphorylation in chloroplasts; inhibition by supraoptimal levels of O2 is considered to be due to photorespiration. Analysis of a range of developmental stages (along the leaf blade and at different leaf ages and positions) showed that the degree of inhibition of photosynthesis by supraoptimal levels of O2 increased rapidly once the ribulose-1,5-bisphosphate carboxylase/oxygenase and chlorophyll contents were below a critical level and was similar to that of C3 plants. Tissue having a high sensitivity of photosynthesis to O2 may be less effective in concentrating CO2 in the bundle sheath cells due either to limited function of the C4 cycle or to higher bundle sheath conductance to CO2. An analysis based on the kinetic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase was used to predict the maximum CO2 level concentrated in bundle sheath cells at a given degree of inhibition of photosynthesis by supraoptimal levels of O2. 相似文献
120.
Conservation of an intact human immunodeficiency virus type 1 vif gene in vitro and in vivo. 总被引:5,自引:5,他引:0 下载免费PDF全文
P Sova M van Ranst P Gupta R Balachandran W Chao S Itescu G McKinley D J Volsky 《Journal of virology》1995,69(4):2557-2564
Replication of vif-negative human immunodeficiency virus type 1 (HIV-1) is attenuated in certain cell lines and highly impaired in peripheral blood lymphocytes in vitro. To determine whether intact vif is positively selected during natural HIV-1 infection and to determine vif sequence variability, we employed PCR amplification, cloning, and sequencing to investigate the vif region of replicating virus in short-term-passage HIV-1 primary isolates from five asymptomatic individuals and from five persons with AIDS. A total of 46 vif clones were obtained and analyzed. Recombinant proviruses were constructed from selected vif clones from one patient and found to be fully infectious. We found that 38 of the 46 clones sequenced carried open vif reading frames and that there was a low degree of heterogeneity of vif genes within isolates from the same individual and among isolates from different donors. The cysteines previously found to be essential for vif protein function were conserved in all clones. A phylogenetic tree constructed from all available vif nucleotide sequences resulted in a virus grouping similar to those of gag and env. Direct sequencing of vif amplified by PCR from uncultured lymphocytes of 15 individuals at various stages of progression toward AIDS demonstrated vif open reading frames in 13 of 15 samples tested. There was no obvious correlation between disease status and the presence of an intact vif within this sample group at the time of sample procurement. The conservation of the vif open reading frame in vitro and in vivo and its limited variability following virus transmission in vitro are consistent with a role for vif in natural HIV-1 infection. 相似文献