抗呼吸道合胞病毒(RSV)单克隆抗体的制备及其对RSV病毒株抗原性的分析

用呼吸道合胞病毒R6(武汉地方株)活毒滴鼻加用戍二醛固定的病毒感染的Hela细胞免疫BALB/C鼠,取脾细胞与小鼠骨髓瘤SP_2/0细胞融合,培育出分泌抗呼吸道合胞病毒单克隆抗体的杂交瘤细胞13株,这些细胞的染色体数为94至104条,其分泌的抗体分别属于鼠IgC_1、IgG_(2a)、IgG_(2b)亚类。腹水荧光抗体滴度为1:10000～1:100000。其中五株单抗有中和病毒作用,尤其是两株中和放价达1:128。应用免疫转印法证实了这些单抗分别能识别RSV6种主要结构蛋白,用7株识别不同病毒结构蛋白的单抗对12株合胞病毒进行抗原性分析,可将这些病毒区分为二个血清型,即A亚型和B亚型。     

地方性克汀病遗传方式的分析

本文通过1794人、176家系,340户的调查,检出地方性克汀病患者64例,应用分离分析和多基因阈值理论,证实本症属多基因遗传,其加权平均遗传率和标准误为78.51±7.53.     

宁夏沙区沙拐枣属和柽柳属的引选及抗逆造林

宁夏分布有大面积的流动沙地和盐碱地,因此,引进适生的植物对治沙造林、改善生态环境有重大意义。本文报道沙拐枣属(Calligonum L.)和柽柳属(Tamarix L.)的引选及抗逆性造林技术方面的结果。     

巴山松天然林生物量和生产力的研究

 经测定分析陕西南部28—37年生巴山松天然林的生物量和净初级生产量,结果表明,林分乔木层生物量为81.28t／ha,草本下木生物量为1.45t／ha,林分凋落物量为11.16t／ha;乔木层净初级生产量为6.94t／ha·yr,约45%分配到树叶。巴山松林的消光系数平均为0.33。在地上部生产结构方面,枝重主要集中在树冠中、下部,叶龄愈大,叶重在树冠下部的比例愈高。巴山松林木的根系总长度约99.4km／ha,其中细根占58%。     

重组白细胞介素-2纯化过程中影响活性的因素探讨

<正> 白细胞介素-2(Interleukin-2,IL-2)是一种重要的免疫调节剂,具有促进T细胞增殖和分化,调节NK细胞活性,诱导LAK等功能,在肿瘤治疗上有广泛的应用前景。我们的前文就重组菌中IL-2包含体的制备、性质及重组rIL-2的纯化进行了研究,本文就重组rIL-2纯化过程中影响其活性的有关因素进行了探讨。     

1,25-双羟胆钙化醇及其受体含量检测的实验研究

 建立了一种改良的血清1,25-双羟胆钙化醇(1,25-Dihydroxycholecalciferol,DHCC)超微量放射受体检测(RRA)技术。完成了灵敏度、精密度、准确度、稳定性及特异性等技术指标。报告了我国健康青年血清DHCC正常值;检测了先天性佝偻病、青春期佝偻病病人及患肾性骨病奶牛等血清DHCC水平。 根据配体与受体相互结合的定量关系,建立了DHCCR(DHCC受体)检测技术。在游离与结合配基分离方面,除建立与比较了DCC(葡聚糖包埋的活性炭)及HAP(羟基磷灰石)方法外,还首次将IEF(等电聚焦电泳)应用于DHCCR分离技术。对佝偻病鸡小肠粘膜上皮细胞受体含量进行了检测并比较了鸡小肠、输卵管壳腺及肝组织DHCCR含量。     

用亲和层析技术分离纯化天冬氨酸酶

<正> 亲和层析技术做为现代生化实验室常用的提纯方法,具有专一性强,活力损失小,分离步骤少等优点。天冬氨酸酶是重要的酶制剂,以往见报的分离纯化工艺步骤繁琐,活力损失大。本文报道的新工艺是用聚乙二醇(PEG)/磷酸钾双水相抽提;DEAE-sophadex A-50柱层析除PEG和部分杂蛋白;环氧氯丙烷活化载体,底物作配基的亲和层析技术获得了电泳纯的天冬氨酸酶。     

Increase in the number of atrial natriuretic hormone receptors in regenerating rat liver.

Forty-eight hours after partial (approximately 67%) hepatectomy the activity of the particulate guanylate cyclase was increased by 2-fold in the regenerating rat liver. This increase was not an artifact of membrane isolation procedures, and as determined by 125I-labeled Tyr-28 atrial natriuretic hormone-(1-28) ANF binding, was accompanied by a 2-fold increase in the number of ANF receptors. The Kd of the receptors in membranes of regenerating livers was not significantly different from the Kd of the receptors in livers of sham-operated rats. The linear synthetic descysteine analog of ANF, analog I, which binds only to the 66-kDa receptors, displaced approximately 40% of the specifically bound 125I-ANF in liver membranes from both hepatectomized and sham-operated (control) animals. Affinity cross-linking studies with 125I-ANF confirmed the increase in the 116-kDa ANF receptor in membranes of regenerating livers. In perfused livers derived from control and hepatectomized animals, the basal rates of cGMP production were not significantly different. However, atriopeptin II-stimulated cGMP production was twice as great in regenerating livers as compared with controls. These data demonstrate that the increase in particulate guanylate cyclase activity observed during liver regeneration is due to an increase in the 116-kDa ANF receptor-associated activity. Additionally, our data demonstrate that the regenerating rat liver may be a valuable model with which to study the role of the hepatic ANF receptor/particulate guanylate cyclase.