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大鲵肝脏组织定向cDNA文库的构建及鉴定 总被引:16,自引:0,他引:16
目前关于大鲵的胚胎发育、繁殖育种及养殖条件的优化等已有不少研究(李峰等,1 998;张育辉等,1 999) ,此外也有一些学者研究了大鲵的习性与生理生化特点(杨国华等,2 0 0 1 ;乔志刚等,2 0 0 3)。但这些研究仍停留在组织和细胞水平,加之大鲵取材来之不易,使研究具有一定的局限性。最近有学者分离了大鲵线粒体基因组全长并完成了测序工作,对于两栖类的遗传多态性研究有重要意义(Zhangetal.,2 0 0 3)。目前有关大鲵的遗传学背景资料仍然缺乏,尚未见特异的大鲵相关基因的报道,相应的遗传学工程课题更是无法开展。随着分子生物学技术的发展和人类… 相似文献
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By homologous EST searching and nested PCR a new human gene GJB5 encoding gap junction protein p-5 was identified. GJB5 was genetically mapped to human chromosome 1p33-p35 by FISH. RT-PCR revealed that it was expressed in skin, placenta and fetal skin. DMA sequencing of GJB5 was carried out in 142 patients with sensorineural hearing impairment and probands of 36 families with genetic diseases, including erythrokeratodermia (5 families), Char-cot-Marie-Tooth disease (13), ptosis (4), and retinitis pigmentosa and deafness (14). Two mis-sense mutations (686A→G, H229R; 25C→T, L9F) were detected in two sensorineural hearing impairment families. A heterologous deletion of 18 bp within intron was found in 3 families with heredity hearing impairment, and in one of the 3 families, a missense mutation (R265P) was identified also. But the deletion and missense mutation seemed not segregating with hearing impairment in the family. No abnormal mRNA or mRNA expression was detected in deletion carriers by RT-PCR anal 相似文献
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记述采自河南和湖南省的叶蜂科突瓣叶蜂亚科2新种:烟翅简栉叶蜂Trichiocampus infumatunus sp.nov.和淡足简栉叶蜂 Trichiocampus femoratinus sp.nov,.编制了简栉叶蜂属中国已知种的分种检索表.新种模式标本保存于中南林业科技大学昆虫模式标本室. 相似文献
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本文记述产于云南省、湖南省和福建省类石蛛两新种:黑色垣蛛,新种Ariadna pelios sp.nov.;敏捷垣蛛,新种Ariadna elaphros sp.nov. 。 相似文献
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Xin Zhao Jingyuan Lu Weiwei Jin a National Maize Improvement Center of China Key Laboratory of Crop Genetic Improvement Genome of Ministry of Agriculture Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing China b College of Bioscience Biotechnology Hunan Agricultural University Changsha China c Opening Lab of Genetic Improvement of Agricultural Crops of Ministry of Agriculture Sino-D... 《Acta Genetica Sinica》2011,(1)
Repetitive DNA sequences with variability in copy number or/and sequence polymorphism can be employed as useful molecular markers to study phylogenetics and identify species/chromosomes when combined with fluorescence in situ hybridization(FISH).Cucumis sativus has three variants,Cucumis sativus L.var.sativus,Cucumis sativus L.var.hardwickii and Cucumis sativus L.var.xishuangbannesis.The phylogenetics among these three variants has not been well explored using cytological landmarks.Here,we concentrate on... 相似文献
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Cadmium is a non-essential metal with a wide distribution that has severe toxic effects on aquatic animals. Changes in hematology and muscle physiology were examined in silver carp (Hypophthalmichthys molitrix) exposed to environmentally relevant levels of cadmium (0.01 mg L-1 ) for 96 h. Cadmium exposure induced significant increases in the red blood cell count, and in the plasma concentrations of cortisol, glucose, and lactate. This suggests that the dose of cadmium was sufficient to cause stress, possibly associated with impaired gas exchange at the gills. There were no changes in hemoglobin concentration or plasma protein concentration. Significant decreases in muscle energy fuels (ATP and glycogen), and increases in muscle lactate persisted until the end of the exposure period, respectively. The changes in muscle lactate and protein in silver carp differed from those observed in response to exposure of fish to cadmium and heavy metals in other studies. The study highlights the importance of selecting unpolluted release sites with suitable water conditions for the survival of newly released individuals for stock enhancement of the Xiangjiang River. 相似文献