全文获取类型
收费全文 | 764篇 |
免费 | 58篇 |
国内免费 | 71篇 |
专业分类
893篇 |
出版年
2024年 | 1篇 |
2023年 | 13篇 |
2022年 | 29篇 |
2021年 | 53篇 |
2020年 | 25篇 |
2019年 | 22篇 |
2018年 | 26篇 |
2017年 | 32篇 |
2016年 | 38篇 |
2015年 | 34篇 |
2014年 | 58篇 |
2013年 | 56篇 |
2012年 | 55篇 |
2011年 | 68篇 |
2010年 | 40篇 |
2009年 | 28篇 |
2008年 | 37篇 |
2007年 | 33篇 |
2006年 | 22篇 |
2005年 | 27篇 |
2004年 | 17篇 |
2003年 | 19篇 |
2002年 | 34篇 |
2001年 | 9篇 |
2000年 | 19篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1987年 | 5篇 |
1986年 | 1篇 |
1985年 | 5篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 4篇 |
1980年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有893条查询结果,搜索用时 15 毫秒
881.
882.
883.
884.
Rapid and efficient selection of recombinant site-directed mutants of Bradyrhizobium japonicum by colony hybridization 总被引:4,自引:0,他引:4
Abstract Due to the high incidence of spontaneous antibiotic resistance and slow growth of Bradyrhizobium japonicum strains, screening for site-directed mutants is cumbersome and time-consuming. A rapid method for selection of recombinant site-directed mutants of B. japonicum was developed. A kanamycin (Km) and a spectinomycin (Sp) cassette were each used to replace DNA fragments in the chromosome by homologous recombination. The primary new features of this method involve a simple plate selection for the antibiotic (Km or Sp) resistant mutants, then colony streaking, and lysis for DNA hybridization on a nitrocellulose filter enabling direct identification of the recombinant site-directed mutants. This method has permitted us to quickly and easily identify a large number of positive recombinant mutants from a large number of indicidual colonies. The procedure eliminates the need to first isolate genomic DNA from each mutant for Southern hybridization. All of the tested site-directed mutants from this method were confirmed to exhibit the expected mutant phenotype. 相似文献
885.
886.
Ping-Chung Liu Kuo-Kau Lee Kah-Ching Yii Guang-Hsiung Kou Shiu-Nan Chen 《Current microbiology》1996,33(2):129-132
Outbreaks of high mortality among the cultured kuruma prawn Penaeus japonicus without overt gross signs occurred during August and December of 1994 in I-Lan, Taiwan. Eleven luminous bacterial strains
were isolated from the hepatopancreas of moribund prawns from five different farms by use of tryptic soy agar (TSA, supplemented
with 2% NaCl) and/or thiosulfate citrate bile salt sucrose (TCBS) agar. These strains, together with our two previously unpublished
isolates, were characterized and identified to be Vibrio harveyi in comparison with two ATCC Type strains and one strain previously isolated from the tiger prawn, P. monodon.
Received: 22 November 1995 / Accepted: 24 February 1996 相似文献
887.
888.
Akara Sasaki Kou Ikejima Shigeru Aoki Nobuyuki Azuma Noboru Kashimura Minoru Wada 《Environmental Biology of Fishes》2003,66(4):307-311
Luminescence of the pony fish, Leiognathus elongatus, was observed in the natural environment during nighttime diving. The light was emitted from the lateroventral portion of the body, as bright rectangular-shaped luminescence patches turned on and off periodically. Luminescent fish had a distinct clear patch on the flank through which light was emitted, whereas non-luminous fish did not have such a clear patch. Both luminous and non-luminous fish were found within a shoal, where non-luminous individuals were chased by luminous ones. From previous morphological studies, the luminous and non-luminous individuals are likely to be male and female, respectively. Our observations provide field evidence that the luminescence functions as intraspecific communications in L. elongatus. 相似文献
889.
S Y Wang S N Chen G H Kou 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1987,11(3):245-252
A cell line, derived from top-minnow (Gambusia patruelis), has been transferred for 114 times in Leibovitz L-15 medium plus 10% FCS (GM-L-15) at a temperature of 31 degrees C and designated as TM. The results of a chromosome analysis of 100 cells at the metaphase stage, show a bimodal distribution of the chromosome number ranged from 21 to 68 with a modal number of 38. At a low seeding density, the plating efficiency of TM cells was 11-12.4%. TM cells are susceptible to EVE, EVA, EVEX, IPNV and LV-1 at 18 degrees C. The result of a sterility test showed that the TM cell line was free of bacterial, fungal and mycoplasmic contamination. The presence of numerous microvilli on the surface of TM cells in the micrographs of normal TM cell. The microvilli are replaced by blebs at the late stage of cytokinesis of the cells. 相似文献
890.
Chun-Mei Zhang Min Zhang Jin-Gai Zhu Chen-Bo Ji Chun Zhu Chun-Zhao Kou Da-Ni Qin Mei-Ling Tong Xi-Rong Guo 《Journal of bioenergetics and biomembranes》2010,42(3):255-259
Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus
far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except
UCP1) in the pathogenesis of obesity. Because of the known cross-reactivity of the antibodies presently available for the
detection of UCP proteins, this study systematically analyzed the differential tissue expression profiles of the 5 UCP isoforms
in lean control mice and ob/ob mice by using real-time polymerase chain reaction (PCR) analysis. The results show that the
tissue-specific expression patterns of individual isoforms in normal and ob/ob mice are considerably different; this will
provide new insights into the functions of UCPs in the pathogenesis of genetic obesity. 相似文献