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Akara Sasaki Kou Ikejima Shigeru Aoki Nobuyuki Azuma Noboru Kashimura Minoru Wada 《Environmental Biology of Fishes》2003,66(4):307-311
Luminescence of the pony fish, Leiognathus elongatus, was observed in the natural environment during nighttime diving. The light was emitted from the lateroventral portion of the body, as bright rectangular-shaped luminescence patches turned on and off periodically. Luminescent fish had a distinct clear patch on the flank through which light was emitted, whereas non-luminous fish did not have such a clear patch. Both luminous and non-luminous fish were found within a shoal, where non-luminous individuals were chased by luminous ones. From previous morphological studies, the luminous and non-luminous individuals are likely to be male and female, respectively. Our observations provide field evidence that the luminescence functions as intraspecific communications in L. elongatus. 相似文献
874.
S Y Wang S N Chen G H Kou 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1987,11(3):245-252
A cell line, derived from top-minnow (Gambusia patruelis), has been transferred for 114 times in Leibovitz L-15 medium plus 10% FCS (GM-L-15) at a temperature of 31 degrees C and designated as TM. The results of a chromosome analysis of 100 cells at the metaphase stage, show a bimodal distribution of the chromosome number ranged from 21 to 68 with a modal number of 38. At a low seeding density, the plating efficiency of TM cells was 11-12.4%. TM cells are susceptible to EVE, EVA, EVEX, IPNV and LV-1 at 18 degrees C. The result of a sterility test showed that the TM cell line was free of bacterial, fungal and mycoplasmic contamination. The presence of numerous microvilli on the surface of TM cells in the micrographs of normal TM cell. The microvilli are replaced by blebs at the late stage of cytokinesis of the cells. 相似文献
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Chun-Mei Zhang Min Zhang Jin-Gai Zhu Chen-Bo Ji Chun Zhu Chun-Zhao Kou Da-Ni Qin Mei-Ling Tong Xi-Rong Guo 《Journal of bioenergetics and biomembranes》2010,42(3):255-259
Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus
far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except
UCP1) in the pathogenesis of obesity. Because of the known cross-reactivity of the antibodies presently available for the
detection of UCP proteins, this study systematically analyzed the differential tissue expression profiles of the 5 UCP isoforms
in lean control mice and ob/ob mice by using real-time polymerase chain reaction (PCR) analysis. The results show that the
tissue-specific expression patterns of individual isoforms in normal and ob/ob mice are considerably different; this will
provide new insights into the functions of UCPs in the pathogenesis of genetic obesity. 相似文献
879.
Airway hyperresponsiveness to bronchoconstrictor challenge after wood smoke exposure in guinea pigs. 总被引:1,自引:0,他引:1
Prior airway exposure to wood smoke induces an increase in airway responsiveness to subsequent smoke inhalation in guinea pigs (Life Sci. 63: 1513, 1998; 66: 971, 2000). To further characterize this airway hyperreactivity, we investigated and compared the airway responsiveness to bronchoconstrictor challenge before and 30 min after sham air exposure or wood smoke exposure in anesthetized and artificially ventilated guinea pigs. Various doses of substance P (0.8-6.4 microg/kg), capsaicin (0.2-3.2 microg/kg), prostaglandin F2alpha (30-3000 microg/kg), histamine (1-8 microg/kg), or acetylcholine (5-20 microg/kg) were intravenously injected at 2-min intervals in successively increasing doses to obtain the dose required to provoke a 200% increase in baseline total lung resistance (ED200). Wood smoke exposure significantly lowered the ED200 of substance P, capsaicin, and prostaglandin F2alpha whereas sham air exposure failed to do so. Furthermore, wood smoke exposure did not significantly alter the ED200 of histamine or acetylcholine. Pretreatment with phosphoramidon (2 mg/kg), an inhibitor of the neutral endopeptidase (the major degradation enzyme of substance P), before smoke exposure did not significantly affect the smoke-induced reduction in ED200 of substance P. Sectioning both cervical vagi before smoke exposure did not significantly alter the smoke-induced reduction in ED200 of capsaicin or prostaglandin F2alpha. These results suggest that airway exposure to wood smoke acutely produces airway hyperresponsiveness to substance P, capsaicin, and prostaglandin F2alpha, but not to histamine or acetylcholine. Since the combination of phosphoramidon and wood smoke exposure did not result in an additive potentiation of smoke-induced airway hyperresponsiveness to substance P, it is suggested that an inhibition of the degradation enzyme of substance P may contribute to this increase in airway reactivity. Furthermore, vagally-mediated bronchoconstriction does not play a vital role in enhanced airway responsiveness to capsaicin or prostaglandin F2alpha. 相似文献