全文获取类型
收费全文 | 280篇 |
免费 | 35篇 |
国内免费 | 32篇 |
专业分类
347篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 9篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 13篇 |
2012年 | 18篇 |
2011年 | 21篇 |
2010年 | 9篇 |
2009年 | 12篇 |
2008年 | 10篇 |
2007年 | 12篇 |
2006年 | 6篇 |
2005年 | 10篇 |
2004年 | 12篇 |
2003年 | 11篇 |
2002年 | 10篇 |
2001年 | 5篇 |
2000年 | 12篇 |
1999年 | 18篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 7篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1987年 | 2篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 9篇 |
1982年 | 3篇 |
1979年 | 2篇 |
1978年 | 7篇 |
1973年 | 3篇 |
1972年 | 6篇 |
1971年 | 3篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1944年 | 1篇 |
1938年 | 2篇 |
排序方式: 共有347条查询结果,搜索用时 8 毫秒
11.
Development of a technology for commercial phytoextraction of nickel: economic and technical considerations 总被引:10,自引:2,他引:10
Li Yin-M. Chaney Rufus Brewer Eric Roseberg Richard Angle J. Scott Baker Alan Reeves Roger Nelkin Jay 《Plant and Soil》2003,249(1):107-115
In recent R&D work, we have made progress in developing a commercial technology using hyperaccumulator plant species to phytoextract nickel (Ni) from contaminated and/or Ni-rich soils. An on-going program is being carried out to develop a genetically improved phytoextraction plant that combines favorable agronomic and Ni accumulation characteristics. Genetically diverse Ni hyperaccumulator species and ecotypes of Alyssum were collected and then evaluated in both greenhouse and field using serpentine and Ni-refinery contaminated soils. Large genetic variation was found in those studies. Mean shoot Ni concentrations in field-grown plants ranged from 4200 to 20400 mg kg–1. We have been studying several soil management practices that may affect the efficiency of Ni phytoextraction. Soil pH is an important factor affecting absorption of metals by plants. An unexpected result of both greenhouse and field experiments was that Ni uptake by two Alyssum species was reduced at lower soil pH and increased at higher soil pH. At higher pH, plant yield was improved also. In soil fertility management studies, we found that N application significantly increased plant biomass, but did not affect plant shoot Ni concentration. These findings indicate that soil management will be important for commercial phytoextraction. A number of field trials have been carried out to study planting methods, population density, weed control practices, harvest schedule and methods, pollination control, and seed processing. Such crop management studies have improved phytoextraction efficiency and provide a tool for farmers to conduct commercial production. We have done some work to develop efficient and cost-effective methods of Ni recovery. Recovery of energy by biomass burning or pyrolysis could help make phytoextraction more cost-effective. The progress made in our recent studies will enable us to apply this technology commercially in the near future. 相似文献
12.
目的:探讨MR弥散加权成像(DWI)鉴别诊断良恶性椎体压缩性骨折的临床价值。方法:对57例经临床或病理证实的椎体良恶性压缩性骨折患者行矢状位T1M、T2WI、T2WI/FS及DWI扫描,研究其在常规序列和DWI序列上的表现,将常规MR序列和DWI序列检出率进行比较,测量正常椎体及病变椎体的表观弥散系数(ADC)值,并进行统计学分析。结果:(1)MR常规序列和DWI序列(b=500s/mm2)表现:良性椎体压缩性骨折呈长T1长或等T2改变,T2WI/FS呈高信号,DWI可以呈高信号、等信号及低信号;恶性椎体压缩性骨折呈长T1长T2信号,大部分病灶T2WUFS及DWI呈高信号,少数变现为低信号;(2)MR常规序列和DWI序列(b=500s/mm2)病灶检出率的比较:T1WI、T2WI/FS及DWI序列病灶检出率均高于T2WI序列,其间的差别有显著性意义(P〈0.01),T1WI、T2WI/FS及DWI序列病灶检出率之间无显著性差异(P〉0.01);(3)ADC值比较:在DWI(b=500s/mm2)上,良性组ADC值为(2.03±0.83)×10^3mm^2/s,恶性组ADC值为(1.37±0.75)×10^-3mm^2/s,正常组ADC值为(0.36±0.21)×10^-3mm^2/s,成像条件相同时,良性组高于恶性组,两组间有明显的统计学意义(P〈0.05)。结论:DWI可较好的反映椎体的弥散特征,ADC值作为量化指标可对良恶性椎体压缩性骨折进行可靠鉴别。 相似文献
13.
S K Mauldin F A Richard M Plescia S D Wyrick A Sancar S G Chaney 《Analytical biochemistry》1986,157(1):129-143
Platinum drugs with the 1,2-diaminocyclohexane (dach) carrier ligand have shown great promise in cancer chemotherapy, but little is known about their metabolism in the body. Since it is possible to radiolabel the dach ligand, it should be possible to quantitate the biotransformation products of these drugs, provided a method were available to separate the biotransformation products. In this paper we describe a two-column high-performance liquid chromatography system which can be used to separate many likely dach-platinum biotransformation products from the parent compounds, and allow their identification. An initial separation on a reverse-phase Partisil ODS-3 column allowed resolution of the uncharged species. The peak fractions from this column were concentrated 10-fold and reinjected onto a cation exchange Partisil 10 SCX column to allow resolution of the positively charged species. This system allowed resolution of two prototype dach-platinum drugs, (cis-1,2-diaminocyclohexane)dichloroplatinum(II) and (cis-1,2-diaminocyclohexane)malonatoplatinum(II), the aquated species likely to form from these drugs, and the complexes formed when these compounds react with glutathione, metallothionein, and amino acids. By using cation exchange chromatography at pH 2.3 as well as pH 4 and by using 14C-labeled amino acids to determine stoichiometry, it was also possible to determine the most likely structures for some of the amino acid complexes. Most importantly, this system allowed clear separation of many of the likely biotransformation products tested from the biologically important aquated species. This system should prove useful for separating and identifying the biotransformation products of dach-platinum drugs in blood and urine, in tissue culture media, and inside the cell. 相似文献
14.
Regulation of mutation rates is critical for maintaining genome stability and controlling cancer risk. A special challenge to this regulation is the presence of multiple mutagenic DNA polymerases in mammals. These polymerases function in translesion DNA synthesis (TLS), an error-prone DNA repair process that involves DNA synthesis across DNA lesions. We found that in mammalian cells TLS is controlled by the tumor suppressor p53, and by the cell cycle inhibitor p21 via its PCNA-interacting domain, to maintain a low mutagenic load at the price of reduced repair efficiency. This regulation may be mediated by binding of p21 to PCNA and via DNA damage-induced ubiquitination of PCNA, which is stimulated by p53 and p21. Loss of this regulation by inactivation of p53 or p21 causes an out of control lesion-bypass activity, which increases the mutational load and might therefore play a role in pathogenic processes caused by genetic instability. 相似文献
15.
16.
17.
Corzo Remigio Amelia Chaney Rufus L. Baker Alan J. M. Edraki Mansour Erskine Peter D. Echevarria Guillaume van der Ent Antony 《Plant and Soil》2020,449(1-2):11-37
Plant and Soil - Phytoextraction is an in situ technique that can be applied to minerals and mining wastes using hyperaccumulator plants to purposely bio-concentrate high levels of metals or... 相似文献
18.
19.
P. Verburg J. Horrox E. Chaney J. C. Rutherford J. M. Quinn R. J. Wilcock C. W. Howard-Williams 《Hydrobiologia》2013,718(1):119-130
Nutrient ratios have been related to nutrient limitation of algal growth in lakes. Retention of nutrients in lakes, by sedimentation and by denitrification, reduces the nutrient concentrations in the water column, thereby enhancing nutrient limitation. Differential retention of nitrogen and phosphorus alters their ratios in lakes and thereby contributes to determine whether nitrogen or phosphorus limits algal growth. We examined the relationships between differential nutrient retention, nutrient ratios, and nutrient limitation in Lake Brunner, a deep oligotrophic lake. The observed retention of nitrogen (20%) and phosphorus (47%) agreed with predictions by empirical equations from literature. As a result of differential retention with a much larger proportion of phosphorus retained than that of nitrogen, the nitrogen:phosphorus ratio was higher in the lake (69) than in the inflows (46). While the mean ratio in the inflows suggested no or only moderate phosphorus limitation, the lake appeared to be severely phosphorus limited. Combining empirical equations from literature that predict nitrogen and phosphorus retention suggests that the nitrogen:phosphorus ratio is enhanced by greater retention of phosphorus compared to nitrogen only in deep lakes with relatively short residence times, such as Lake Brunner. In contrast, in most lakes differential retention is expected to result in lower nitrogen:phosphorus ratios. 相似文献
20.