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101.
Gang Li Tianming Liu Ashley Tarokh Jingxin Nie Lei Guo Andrew Mara Scott Holley Stephen TC Wong 《BMC cell biology》2007,8(1):40
Background
Reliable segmentation of cell nuclei from three dimensional (3D) microscopic images is an important task in many biological studies. We present a novel, fully automated method for the segmentation of cell nuclei from 3D microscopic images. It was designed specifically to segment nuclei in images where the nuclei are closely juxtaposed or touching each other. The segmentation approach has three stages: 1) a gradient diffusion procedure, 2) gradient flow tracking and grouping, and 3) local adaptive thresholding. 相似文献102.
Background
Automated identification of cell cycle phases of individual live cells in a large population captured via automated fluorescence microscopy technique is important for cancer drug discovery and cell cycle studies. Time-lapse fluorescence microscopy images provide an important method to study the cell cycle process under different conditions of perturbation. Existing methods are limited in dealing with such time-lapse data sets while manual analysis is not feasible. This paper presents statistical data analysis and statistical pattern recognition to perform this task. 相似文献103.
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Hong Zhao Jinmin Zhu Kemi Cui Xiaoyin Xu Megan O'Brien Kelvin K Wong Santosh Kesari Weiming Xia Stephen TC Wong 《Cancer cell international》2009,9(1):15
Background
Cancer and Alzheimer's disease (AD) are two seemingly distinct diseases and rarely occur simultaneously in patients. To explore molecular determinants differentiating pathogenic routes towards AD or cancer, we investigate the role of amyloid β protein (Aβ) on multiple tumor cell lines that are stably expressing luciferase (human glioblastoma U87; human breast adenocarcinoma MDA-MB231; and mouse melanoma B16F). 相似文献108.
Isbell LA Young TP Jaffe KE Carlson AA Chancellor RL 《International journal of primatology》2009,30(1):103-124
Mortality patterns are thought to be strong selective forces on life history traits, with high adult mortality and low immature
mortality favoring early and rapid reproduction. Patas monkeys (Erythrocebus patas) have the highest potential rates of population increase for their body size of any haplorhine primate because they reproduce
both earlier and more often. We report here 10 yr of comparative demographic data on a population of patas monkeys and a sympatric
population of vervet monkeys (Cercopithecus aethiops), a closely related species differing in aspects of social system, ecology, and life history. The data reveal that 1) adult
female patas monkeys have significantly higher mortality than adult female vervets; 2) infant mortality in patas monkeys is
relatively low compared to the norm for mammals because it is not significantly different from that of adult female patas
monkeys; and 3) infant mortality is significantly higher than adult female mortality in vervets. For both species, much of
the mortality could be attributed to predation. An epidemic illness was also a major contributor to the mortality of adult
female patas monkeys whereas chronic exposure to pathogens in a cold and damp microenvironment may have contributed to the
mortality of infant vervets. Both populations experienced large fluctuations during the study period. Our results support
the prediction from demographic models of life history evolution that high adult mortality relative to immature mortality
selects for early maturation. 相似文献
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Marc C. Levesque Maurine R. Hobbs Charles W. O’Loughlin Jennifer A. Chancellor Youwei Chen Ariana N. Tkachuk Jennifer Booth Kistie B. Patch Sallie Allgood Ann R. Pole Carolyn A. Fernandez Esther D. Mwaikambo Theonest K. Mutabingwa Michal Fried Bess Sorensen Patrick E. Duffy Donald L. Granger Nicholas M. Anstey J. Brice Weinberg 《Human genetics》2010,127(2):163-182
Nitric oxide (NO) mediates host resistance to severe malaria and other infectious diseases. NO production and mononuclear cell expression of the NO producing enzyme-inducible nitric oxide synthase (NOS2) have been associated with protection from severe falciparum malaria. The purpose of this study was to identify single nucleotide polymorphisms (SNPs) and haplotypes in the NOS2 promoter, to identify associations of these haplotypes with malaria severity and to test the effects of these polymorphisms on promoter activity. We identified 34 SNPs in the proximal 7.3 kb region of the NOS2 promoter and inferred NOS2 promoter haplotypes based on genotyping 24 of these SNPs in a population of Tanzanian children with and without cerebral malaria. We identified 71 haplotypes; 24 of these haplotypes comprised 82% of the alleles. We determined whether NOS2 promoter haplotypes were associated with malaria severity in two groups of subjects from Dar es Salaam (N = 185 and N = 250) and in an inception cohort of children from Muheza-Tanga, Tanzania (N = 883). We did not find consistent associations of NOS2 promoter haplotypes with malaria severity or malarial anemia, although interpretation of these results was potentially limited by the sample size of each group. Furthermore, cytokine-induced NOS2 promoter activity determined using luciferase reporter constructs containing the proximal 7.3 kb region of the NOS2 promoter and the G-954C or C-1173T SNPs did not differ from NOS2 promoter constructs that lacked these polymorphisms. Taken together, these studies suggest that the relationship between NOS2 promoter polymorphisms and malaria severity is more complex than previously described. 相似文献