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51.
Kalinichenko EN Podkopaeva TL Mikhaĭlopulo IA Kelve M Saarma M van den Boogaart J Altona C 《Bioorganicheskaia khimiia》1999,25(10):763-767
Mouse antibodies to (2'-5')oligoadenylates were obtained by the immunization of animals with the (2'-5')oligoadenylic acid trimer conjugated with bovine serum albumin through a 2',3'-levulinic acid residue. Using radioimmunoassay, the reactivity of mouse polyclonal antibodies to the (2'-5')oligoadenylic acid trimer was studied for the trimer analogues containing 9-(3-deoxy-3-fluro-beta-D- xylofuranosyl)adenine and 3'-deoxy-3'-fluoro-adenosine in various positions of the chain. It was found that (a) the three-dimensional structure of short oligonucleotides is an important factor in the antibody recognition; (b) antibodies are more sensitive to modifications of the 5'-terminal and central ribose fragments of the (2'-5')oligoadenylic acid trimer; (c) the 3'-hydroxyl group plays a secondary role in the formation of the antigen determinant. 相似文献
52.
Kovalenko OP Petrushenko ZM Kriklivyĭ IA Iaremchuk AD Tukalo MA 《Bioorganicheskaia khimiia》1999,25(10):768-773
The reactivity of phosphates in the Thermus thermophilus tRNA(Ser) (GCU) and tRNA(Leu) (CAG) was studied using the ethylnitrosourea modification. It was shown that phosphates of nucleotides 58-60 (T loop), 20-22 (D loop), and 48 (at the junction of the variable and T stems) were poorly modified in both tRNAs. The most pronounced differences in the reactivity were observed for phosphates at the junctions of the variable stem with T-stem (47q, 49) and anticodon stem (45). This indicates differences in orientations of the long variable arm relative to the backbone in the tRNAs studied. 相似文献
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The research shows some biochemical indices in ontogenesis in broiler hen skin tissues and also its reaction to inner introduction of different doses of radioactive 137Cs. Namely, the irradiation ionizing irradiation influences actively to the indices of nuclein changes with 1 day and 1 week aged chickens. The quantity of phosphorus of nuclein acids is less with the chickens of the third group (500 Bk pr day) than with the second group (3000 Bk/day). The quantity of soluble protein, activity of the estimated fragments, the quantity of inorganic phosphorus, the concentration of ions of magnesium with chickens of the controlled group is larger than in the studied ones. It proves the influence of radionucleid 137Cs on indices which characterize the protein-nuclein change. 相似文献
55.
Antoniak HL Snityns'kyĭ VV Babych NO Iskra RIa Buchko OM 《Ukrainski? biokhimicheski? zhurnal》1999,71(3):44-50
The age changes of enzymes of activity catalyzing several links of energy metabolism (hexokinase, phosphofructokinase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, NADP-isocitrate dehydrogenase, cytochrome c-oxidase) and antioxidant system (superoxide dismutase and glutathione reductase) in bone marrow myeloid cells and blood leukocytes of pig in the 10-day period after birth were investigated. The bone marrow cells and leukocytes of the new born piglets were characterized by low intensity of oxidative steps of energy metabolism as well as by low activity of antioxidant enzymes. In the period of neonatal adaptation reorganization of energy metabolism, particularly, intensification of oxidative processes in the investigated cells occurred. It included the pentose phosphate way and cytochrome c-oxidase activation. During the neonatal period of development the functional activity of antioxidant enzymes in the investigated cells of piglets increased. 相似文献
56.
Starodub VM Fedorenko LL Sisets'kyĭ AP Kurs'kyĭ MD 《Ukrainski? biokhimicheski? zhurnal》1999,71(3):68-72
For the first time, the photoluminescence of porous silicon was used to create an immune sensor capable of detection of low concentrations of myoglobin important for the diagnostics of heart failure diseases which in both model solutions and human serum, being. It is shown that the sensitivity of the sensor was 10 ng/ml, time of 1 measurement--15-30 min. Duration of the analysis can be even shorter if measurements are performed in the kinetic regime. The comparison of the characteristics of the immune sensor and standard method ELISA is also given in the paper. 相似文献
57.
Gubskiĭ IuI Goriushko AG Litvinova NV Pirmak RG Kruskaia NM Shnurko EV Sachenko LG 《Ukrainski? biokhimicheski? zhurnal》1999,71(4):35-39
Interaction of alpha-tocopherol and 1,4-dihydropyridine with endoplasmic reticulum membranes and model systems, human serum albumin and phospholipid bilayer, was studied using the microcalorimetry and fluorescent probes procedures. Dependence of microviscosity changes in the endoplasmic reticulum membranes on the place of antioxidants localization (protein structures or phospholipid phase) was shown. Increase of membrane structuralization under the influence of 1,4-dihydropyridines blocked their antioxidant action in spontaneous and induced lipid peroxidation. 相似文献
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