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971.
Guilherme O. Barbosa Juliete A. F. Silva Aline Siqueira-Berti Umar Nishan Rafaela Rosa-Ribeiro Silvia B. P. Oliveira Mariana O. Baratti Danilo Ferrucci Julio C. O. Santana Danilo M. Damas-Souza Alexandre Bruni-Cardoso Taize M. Augusto Felipe Corrêa-da-Silva Pedro M. Moraes-Vieira Dagmar R. Stach-Machado Sergio L. Felisbino Gustavo B. Menezes Carlos L. Cesar Hernandes F. Carvalho 《Journal of cellular physiology》2019,234(10):19048-19058
Prostate development and function are regulated by androgens. Epithelial cell apoptosis in response to androgen deprivation is caspase-9-dependent and peaks at Day 3 after castration. However, isolated epithelial cells survive in the absence of androgens. Znf142 showed an on-off expression pattern in intraepithelial CD68-positive macrophages, with the on-phase at Day 3 after castration. Rats treated with gadolinium chloride to deplete macrophages showed a significant drop in apoptosis, suggesting a causal relationship between macrophages and epithelial cell apoptosis. Intraepithelial M1-polarization was also limited to Day 3, and the inducible nitric oxide synthase (iNOS) knockout mice showed significantly less apoptosis than wild-type controls. The epithelial cells showed focal DNA double-strand breaks (DSB), 8-oxoguanine, and protein tyrosine-nitrosylation, fingerprints of exposure to peroxinitrite. Cultured epithelial cells induced M1-polarization and showed focal DSB and underwent apoptosis. The same phenomena were reproduced in LNCaP cells cocultured with Raw 264.7 macrophages. In conclusion, the M1 142-macrophage (named after Znf142) attack causes activation of the intrinsic apoptosis pathway in epithelial cells after castration. 相似文献
972.
Ying Zhang Longhui Ma Changhong Ren Kaiyin Liu Xin Tian Di Wu Yuchuan Ding Junfa Li Cesar V. Borlongan Xunming Ji 《Journal of cellular physiology》2019,234(8):12637-12645
Remote ischemic postconditioning (RIPC) is a promising neuroprotective strategy for ischemic stroke. Here, we employed a focal ischemic stroke mouse model to test the hypothesis that poststroke collateral circulation as a potent mechanism of action underlying the therapeutic effects of immediate RIPC. During reperfusion of cerebral ischemia, the mice were randomly assigned to receive RIPC, granulocyte colony-stimulating factor (G-CSF) as a positive control, or no treatment. At 24 hr, we found RIPC and G-CSF increased monocytes/macrophages in the dorsal brain surface and in the spleen, coupled with enhanced leptomeningeal collateral flow compared with nontreatment group. Blood monocytes depletion by 5-fluorouracil (5-FU) significantly limited the neuroprotection of RIPC or G-CSF treatment. The protein expression of proangiogenic factors such as Ang-2 was increased by ischemia, but treatment with either RIPC or G-CSF showed no further upregulation. Thus, immediate RIPC confers neuroprotection, in part, by enhancing leptomeningeal collateral circulation in a mouse model of ischemic stroke. 相似文献
973.
974.
Alexandre Pires Marceniuk Tibrio Cesar Tortola Burlamaqui Claudio Oliveira Jeferson Carneiro Bruno Eleres Soares Joo Brullio de Luna Sales 《Journal of Zoological Systematics and Evolutionary Research》2019,57(2):400-417
The Atlantic coast of South America is characterized by significant variation in environmental attributes, such as the salinity and transparency of the water, substrate, temperatures, and oceanic currents, which contribute to the existence of heterogeneous environments with distinct biotic and abiotic conditions. The present study shows that the Aspistor quadriscutis from the equatorial Amazon–Orinoco plume and Aspistor luniscutis from the northeast to southeast coast of Brazil represent different morphological lineages, with distinct life habits and behavior, belonging to a single genetic lineage. This condition is related to the distinct selective pressures (ecological conditions) found in the geographic regions in which the two species occur. Intermediate morphological patterns correspond to the ancestral traits, present in fossil species, which, together with the absence of differentiation in mitochondrial markers, characterize a process of incipient speciation. 相似文献
975.
de Lucena Marcos B. Cordeiro Cesar A. M. M. Valentin Jean L. Aguiar Aline A. 《Environmental Biology of Fishes》2022,105(5):561-573
Environmental Biology of Fishes - Invertivorous fish species such as Halichoeres radiatus feed on benthic active prey and need to spend most of their time foraging. Even though the Labridae family... 相似文献
976.
977.
The symbiosis between legumes and rhizobia is characterised by the formation of dinitrogen-fixing root nodules. In natural
conditions, nitrogen fixation is strongly impaired by abiotic stresses which generate over-production of reactive oxygen species.
Since one of the nodule main antioxidant systems is the ascorbate–glutathione cycle, NADPH recycling that is involved in glutathione
reduction is of great relevance under stress conditions. NADPH is mainly produced by glucose 6-phosphate dehydrogenase (G6PDH;
EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44) from the oxidative pentose phosphate pathway, and also
by NADP+-dependent isocitrate dehydrogenase (ICDH; EC 1.1.1.42). In this work, 10 μM paraquat (PQ) was applied to pea roots in order
to determine the in vivo relationship between oxidative stress and the activity of the NADPH-generating enzymes in nodules.
Whereas G6PDH and 6PGDH activities remained unchanged, a remarkable induction of ICDH gene expression and a dramatic increase
of the ICDH activity was observed during the PQ treatment. These results support that ICDH has a key role in NADPH recycling
under oxidative stress conditions in pea root nodules. 相似文献
978.
Mitochondrial function and nitric oxide metabolism are modified by enalapril treatment in rat kidney
Piotrkowski B Fraga CG de Cavanagh EM 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,292(4):R1494-R1501
The renal and cardiac benefits of renin-angiotensin system (RAS) inhibition in hypertension exceed those attributable to blood pressure reduction, and seem to involve mitochondrial function changes. To investigate whether mitochondrial changes associated with RAS inhibition are related to changes in nitric oxide (NO) metabolism, four groups of male Wistar rats were treated during 2 wk with a RAS inhibitor, enalapril (10 mg x kg(-1) x day(-1); Enal), or a NO synthase (NOS) inhibitor, N(omega)-nitro-L-arginine methyl ester (L-NAME) (1 mg x kg(-1) x day(-1)), or both (Enal+L-NAME), or were untreated (control). Blood pressure and body weight were lower in Enal than in control. Electron transfer through complexes I to III and cytochrome oxidase activity were significantly lower, and uncoupling protein-2 content was significantly higher in kidney mitochondria isolated from Enal than in those from control. All of these changes were prevented by L-NAME cotreatment and were accompanied by a higher production/bioavailability of kidney NO. L-NAME abolished mitochondrial NOS activity but failed to inhibit extra-mitochondrial kidney NOS, underscoring the relevance of mitochondrial NO in those effects of enalapril that were suppressed by L-NAME cotreatment. In Enal, kidney mitochondria H(2)O(2) production rate and MnSOD activity were significantly lower than in control, and these effects were not prevented by L-NAME cotreatment. These findings may clarify the role of NO in the interactions between RAS and mitochondrial metabolism and can help to unravel the mechanisms involved in renal protection by RAS inhibitors. 相似文献
979.
980.
Oscar Fernando Mikery Pacheco Julio Cesar Rojas León Eduardo Alfonso Rebollar-Téllez Alfredo Castillo Vera 《Memórias do Instituto Oswaldo Cruz》2015,110(1):142-144
Monitoring phlebotomine sandflies in urban areas is key for epidemiological studies
in susceptible populations. This paper describes sandfly fauna that were present in
an urban area of the municipality of Tapachula, Chiapas, Mexico, and were captured
with Shannon and CDC light traps. During February and March of 2014, 1,442 sandflies
were captured, specifically Lutzomyia cruciata (Coquillet) (98.8%), Lutzomyia
cayennensis cayennensis (Floch and Abonnenc) (0.8%), Lutzomyia chiapanensis (Dampf)
(0.3%) and Lutzomyia atulapai (De León) (0.1%). Lu. cruciata was the most abundant
and the most frequently trapped species. This is the first record of its remarkable
ability to adapt to urban green areas. The three other species trapped represent new
records of geographic distribution for the study region. These results indicate the
need to establish measures for reducing both human contact with this vector and the
risk of possible sites of infection. 相似文献