全文获取类型
收费全文 | 5512篇 |
免费 | 619篇 |
国内免费 | 1篇 |
专业分类
6132篇 |
出版年
2021年 | 58篇 |
2020年 | 44篇 |
2019年 | 57篇 |
2018年 | 77篇 |
2017年 | 50篇 |
2016年 | 81篇 |
2015年 | 152篇 |
2014年 | 175篇 |
2013年 | 229篇 |
2012年 | 286篇 |
2011年 | 279篇 |
2010年 | 183篇 |
2009年 | 165篇 |
2008年 | 235篇 |
2007年 | 222篇 |
2006年 | 202篇 |
2005年 | 217篇 |
2004年 | 196篇 |
2003年 | 198篇 |
2002年 | 211篇 |
2001年 | 200篇 |
2000年 | 177篇 |
1999年 | 150篇 |
1998年 | 75篇 |
1997年 | 61篇 |
1996年 | 59篇 |
1995年 | 45篇 |
1994年 | 56篇 |
1993年 | 49篇 |
1992年 | 111篇 |
1991年 | 106篇 |
1990年 | 86篇 |
1989年 | 110篇 |
1988年 | 92篇 |
1987年 | 95篇 |
1986年 | 75篇 |
1985年 | 92篇 |
1984年 | 59篇 |
1983年 | 65篇 |
1982年 | 53篇 |
1981年 | 49篇 |
1980年 | 54篇 |
1979年 | 69篇 |
1978年 | 50篇 |
1977年 | 72篇 |
1976年 | 50篇 |
1975年 | 49篇 |
1974年 | 42篇 |
1973年 | 44篇 |
1971年 | 39篇 |
排序方式: 共有6132条查询结果,搜索用时 15 毫秒
41.
42.
43.
Robert S. Aronstam Michael W. Edwards John W. Daly X. Albuquerque 《Neurochemical research》1988,13(2):171-176
The interactions of eight piperidine derivatives with nicotinic receptor complexes fromTorpedo californica electric organ were studied using [125I]alpha-bungarotoxin ([125I]BGT) as a probe for the acetylcholine binding site and [3H]perhydrohistrionicotoxin ([3H]H12-HTX) as a probe for a site associated with the receptor-gated ion channel.Cis- andtrans-2-methyl-6-n-undecanyl piperidines (MUP), major constituents of fire ant venom, had a high-affinity for [3H]H12-HTX binding sites (Ki=0.08–0.24 M), but had no affect on receptor binding. MUP affinity for [3H]H12-HTX binding sites was approximately doubled in the presence of 1 M carbamylcholine. Introduction of a 2-hydroxyl group to the undecanyl side channel had little effect on activity of the alkaloid. The analog 2,6- (but not 3,5-) dimethylpiperidine was a moderately active inhibitor of [3H]H12-HTX binding (K
i-8.8 M). 2-Methylpiperidine was considerably less active (K
i=600 M), although it was more potent than either 3- or 4-methylpiperidine. The affinities of 2,6-dimethylpiperidine and 2-methylpiperidine for [3H]H12-HTX binding sites were decreased in the presence of 1 M carbamylcholine. Carbamylcholine affinity for the receptor was increased by up to 7 fold in the presence of 10 and 32 M MUP, but was decreased in the presence of 2,6-dimethylpiperidine and 2-methylpiperidine. Thecis- andtrans-isomers of MUP were equipotent in producing each of its effects. In these actions, MUP resembles a variety of other compounds derived from 2,6-disubstituted piperidines, including histrionicotoxins, gephyrotoxins and pumiliotoxins. These studies establish the importance of alkyl substitutions in theortho position of the piperidine ring in conferring ion channel specificity, and the importance of substantial alkyl side chains in conferring the ability of channel blockers to stabilize the nicotinic receptor complex in high affinity, desensitized conformations. 相似文献
44.
The effects of cultural conditions on growth and secondary metabolism in Streptomyces thermoviolaceus 总被引:2,自引:0,他引:2
Abstract Granaticin, an isochromate quinone antibiotic is synthesized as a secondary metabolite by Streptomyces thermoviolaceus . Antibiotic productivity was investigated under a variety of cultural conditions, including complex and defined media, mesophilic and thermophilic temperatures and a variety of sole carbon sources. In a defined medium growth was supported, to varying extents, by different carbon sources and in most cases granaticin production was observed. Highest biomass and granaticin yields were obtained when cultures were grown in the presence of xylan, fructose, glutamate or proline as carbon source. Changes in pH during growth affected both the timing and extent of granaticin production. 相似文献
45.
Analysis of a transformed cell line using antisense c-fos RNA 总被引:2,自引:0,他引:2
Simian sarcoma virus (SSV)-infected NIH-3T3 cells (SSV-NIH-3T3), express a homologue of platelet-derived growth factor, (PDGF) a powerful inducer of the c-fos gene. We have used these cells to test the hypothesis that autocrine stimulation by PDGF-like molecules leads to c-fos expression which is functional in the transformed phenotype. We have transfected SSV-NIH-3T3 cells with a c-fos antisense-RNA expression vector, pSVsof, or control plasmids. pSVsof-transfected cells exhibit markedly decreased c-fos mRNA and protein levels, restored density-dependent growth arrest and reduced (three of five clones) tumorigenicity compared to control lines. The results confirm that c-fos cooperates in the transformed phenotype of SSV-NIH-3T3 cells. 相似文献
46.
Age structure of nematode populations around maize growing in sandy soils in Iowa was studied at soil depths of 0-15and 15-30 cm for 2 years. Numbers of Longidorus breviannulatus were generally greater at 0-15 cm than at 15-30 cm deep until mid to late season. The decline in numbers of females as the season progressed indicates that fecundity slowed and is evidence of only one generation per year. Peak populations of Pratylenchus scribneri and Xiphinema americanum occurred in late August or early September. Adults of Hoplolaimus galeatus were few in the roots but common in the soil, indicating that fertilization occurred mostly in the soil. Numbers of P. scribneri were generally greater at the lower depth, especially late in the season. Community diversity (H'') was less when nematode biomass was used instead of numbers. Numbers of H. galeatus did not decline over the winter. Numbers of L. breviannulatus, P. scribneri, and X. americanum declined significantly over the winter, but not between spring cultivation and planting. 相似文献
47.
I Motelica-Heino M Castanier P Corbier D A Edwards J Roffi 《Journal of steroid biochemistry》1988,31(3):283-286
Newborn female and male C57BL6 mice were decapitated at birth or at different times during the first 24 h after birth and testosterone was determined by radioimmunoassay in plasma and testes. In newborn females, plasma testosterone is low and does not significantly change over the first 24 h after birth. In contrast, in newborn males, plasma testosterone more than doubles during the first 2 h after birth and then falls rapidly to remain relatively low for the remainder of the 24 h period after birth. The increase in plasma testosterone is of almost certain testicular origin since it follows a decrease in testicular testosterone content. It seems likely that the increase in plasma testosterone in male mice which reaches its peak at 2 h after birth is involved in an essential way in the development of well-documented sex differences in gonadotropin secretion and behavior. 相似文献
48.
An antiserum to glutathione S-transferase (EC 2.5.1.18) from maize (Zea mays L.) responsible for herbicide detoxification has been raised in rabbit. The antiserum was specific to the Mr 26000 subunit of the enzyme from maize seedlings and suspension-cultured cells, and recognized the isoenzymes active toward both atrazine and metolachlor. When plants were treated for 24 h with the herbicide antidote N,N-diallyl-2-2-dich-loroacetamide (DDCA), enzyme activities toward metolachlor were doubled in the roots and this was associated with a 70% increase in immunodetectable protein. Translation of polysomal RNA in vitro showed that the increase in the transferase in root tissue was brought about by a ninefold increase in mRNA activity encoding the enzyme. Treatment of suspension-cultured cells with cinnamic acid, metolachlor and DDCA raised enzyme activities but did not increase synthesis of glutathione S-transferase. In cultured maize cells, enzyme synthesis was maximal in mid-logarithmic phase, coinciding with the highest levels of enzyme activity. When callus cultures were established from the shoots of a maize line known to conjugate chloro-s-triazines, enzyme activity towards atrazine was lost during primary dedifferentiation. However, levels of total immunodetectable enzyme and activity toward metolachlor were increased in cultured cells compared with the parent shoot tissue. 相似文献
49.
Though the physiological role of specific binding proteins in serum is unknown, and the validity of the "free hormone hypothesis" has been challenged by several authors (including ourselves), we regard Pardridge's widely publicised objections to this hypothesis (and his recent suggestion of the existence of complex biochemical mechanisms causing the release of individual hormones in particular tissues) as deriving from an incorrect analysis of the effects of serum binding proteins on the kinetics of hormone transport, and therefore without foundation. This presentation is intended to substantiate this view. 相似文献
50.