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Scrotal infrared digital thermography as a predictor of seasonal effects on sperm traits in Braford bulls 总被引:1,自引:0,他引:1
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Keity Souza Santos Marco Antonio Stephano José Roberto Marcelino Virginia Maria Resende Ferreira Thalita Rocha Celso Caricati Hisako Gondo Higashi Ana Maria Moro Jorge Elias Kalil Osmar Malaspina Fabio Fernandes Morato Castro Mário Sérgio Palma 《PloS one》2013,8(11)
Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)´2-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time. 相似文献
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Eutimio Gustavo Fernández Núñez Jaci Leme Letícia de Almeida Parizotto Alexandre Gonçalves de Rezende Bruno Labate Vale da Costa Vera Lucia Lopes Boldorini Soraia Attie Calil Jorge Renato Mancini Astray Carlos Augusto Pereira Celso Pereira Caricati Aldo Tonso 《Cytotechnology》2016,68(1):95-104
Mammalian cells are the most frequently used hosts for biopharmaceutical proteins manufacturing. Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation expansion process is to generate large volume of viable cells in the shortest time. The aim of this paper was to optimize the inoculum preparation stage of baby hamster kidney (BHK)-21 cells for suspension cultures in benchtop bioreactors, by means of a combination of static and agitated culture systems. Critical parameters for static (liquid column height: 5, 10, 15 mm) and agitated (working volume: 35, 50, 65 mL, inoculum volume percentage: 10, 30 % and agitation speed: 25, 60 rpm) cultures were study in T-flask and spinner flask, respectively. The optimal liquid column height was 5 mm for static culture. The maximum viable cell concentration in spinner flask cultures was reached with 50 mL working volume and the inoculum volume percentage was not significant in the range under study (10–30 %) at 25 rpm agitation. Agitation speed at 60 rpm did not change the main kinetic parameters with respect to those observed for 25 rpm. These results allowed for a schedule to produce more than 4 × 109 BHK-21 cells from 4 × 106 cells in 13 day with 1,051 mL culture medium. 相似文献
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Human tracks discovered in 1874 at a site named El Cauce or Acahualinca near the shores of Lake Managua, Nicaragua, are the most famous and abundant human tracks in the Americas. They represent a landmark ichnological discovery during the late 19th century that generated much debate regarding their age and origins. Reported dates for the tracks range from 2,120 to 6,500 B.P. The site, which is now situated within the limits of Nicaragua's capital city of Managua, forms the basis of the Acahualinca Tracks Museum (Huellas de Acahualinca) and has been in place since 1953. However, it is still little known and has not been systematically studied, despite being an important window into Meso-American prehistory. Two exposures of a surface of volcanic ash reveal hundreds of human tracks comprising a minimum of 12 clearly defined trackways, and a trampled zone or path representing at least three more individuals, all heading in the same northwesterly direction. Tracks of deer, opossum and at least one bird are also present. Bison tracks collected from another nearby site (El Recreo) that is no longer accessible are on display at the museum. The Acahualinca tracks are noteworthy for the exceptional quality of preservation. We herein present the first maps of the two exposures, which combine to tell a story of human and animal activity along the shores of ancient Lake Managua several thousand years ago. 相似文献
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Fernando Espinoza 《Science activities》2013,50(3):9-17
This article describes an activity that integrates both mathematics and science while inviting students to make connections between the two and learn significant concepts in a meaningful way. Students work within the real-world context of wildlife population scenarios to make predictions, test their hypotheses, and determine and construct graphs that best represent their data while learning the importance of animal habitats and the factors that impact wildlife populations in continually changing ecosystems. Discussion and experiences with students prior to this activity should ensure that they understand ideas about carrying capacity, components of habitat, and limiting factors. 相似文献