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21.
Xia M Hou C Demong D Pollack S Pan M Brackley J Singer M Matheis M Cavender D Wachter M 《Bioorganic & medicinal chemistry letters》2008,18(12):3562-3564
The synthesis and biological evaluation of a series of substituted dipiperidine alcohols are described. Structure-activity relationship studies led to the discovery of potent CCR2 antagonists displaying IC(50) values in the nanomolar or subnanomolar range. The cinnamoyl compounds had higher binding affinities than the corresponding urea analogs. 相似文献
22.
Michael A Haq S Chen X Hsich E Cui L Walters B Shao Z Bhattacharya K Kilter H Huggins G Andreucci M Periasamy M Solomon RN Liao R Patten R Molkentin JD Force T 《The Journal of biological chemistry》2004,279(20):21383-21393
Glycogen synthase kinase (GSK) 3beta is a negative regulator of stress-induced cardiomyocyte hypertrophy. It is not clear, however, if GSK-3beta plays any role in regulating normal cardiac growth and cardiac function. Herein we report that a transgenic mouse expressing wild type GSK-3beta in the heart has a dramatic impairment of normal post-natal cardiomyocyte growth as well as markedly abnormal cardiac contractile function. The most striking phenotype, however, is grossly impaired diastolic relaxation, which leads to increased filling pressures of the left ventricle and massive atrial enlargement. This is due to profoundly abnormal calcium handling, leading to an inability to normalize cytosolic [Ca2+] in diastole. The alterations in calcium handling are due at least in part to direct down-regulation of the sarcoplasmic reticulum calcium ATPase (SERCA2a) by GSK-3beta, acting at the level of the SERCA2 promoter. These studies identify GSK-3beta as a regulator of normal growth of the heart and are the first of which we are aware, to demonstrate regulation of expression of SERCA2a, a critical determinant of diastolic function, by a cytosolic signaling pathway, the activity of which is dynamically modulated. De-regulation of GSK-3beta leads to severe systolic and diastolic dysfunction and progressive heart failure. Because down-regulation of SERCA2a plays a central role in the diastolic and systolic dysfunction of patients with heart failure, these findings have potential implications for the therapy of this disorder. 相似文献
23.
Raffaella Ponassi Barbara Biasotti Valeria Tomati Silvia Bruno Alessandro Poggi Davide Malacarne Guido Cimoli Annalisa Salis Sarah Pozzi Maurizio Miglino Gianluca Damonte Pietro Cozzini Francesca Spyrakis Barbara Campanini Luca Bagnasco Nicoletta Castagnino Lorenzo Tortolina Anna Mumot Francesco Frassoni Antonio Daga Michele Cilli Federica Piccardi Ilaria Monfardini Miriam Perugini Gabriele Zoppoli Cristina D’Arrigo Raffaele Pesenti Silvio Parodi 《Cell cycle (Georgetown, Tex.)》2012,11(19):3703
24.
Qayyum R Snively BM Ziv E Nalls MA Liu Y Tang W Yanek LR Lange L Evans MK Ganesh S Austin MA Lettre G Becker DM Zonderman AB Singleton AB Harris TB Mohler ER Logsdon BA Kooperberg C Folsom AR Wilson JG Becker LC Reiner AP 《PLoS genetics》2012,8(3):e1002491
Several genetic variants associated with platelet count and mean platelet volume (MPV) were recently reported in people of European ancestry. In this meta-analysis of 7 genome-wide association studies (GWAS) enrolling African Americans, our aim was to identify novel genetic variants associated with platelet count and MPV. For all cohorts, GWAS analysis was performed using additive models after adjusting for age, sex, and population stratification. For both platelet phenotypes, meta-analyses were conducted using inverse-variance weighted fixed-effect models. Platelet aggregation assays in whole blood were performed in the participants of the GeneSTAR cohort. Genetic variants in ten independent regions were associated with platelet count (N?=?16,388) with p<5×10(-8) of which 5 have not been associated with platelet count in previous GWAS. The novel genetic variants associated with platelet count were in the following regions (the most significant SNP, closest gene, and p-value): 6p22 (rs12526480, LRRC16A, p?=?9.1×10(-9)), 7q11 (rs13236689, CD36, p?=?2.8×10(-9)), 10q21 (rs7896518, JMJD1C, p?=?2.3×10(-12)), 11q13 (rs477895, BAD, p?=?4.9×10(-8)), and 20q13 (rs151361, SLMO2, p?=?9.4×10(-9)). Three of these loci (10q21, 11q13, and 20q13) were replicated in European Americans (N?=?14,909) and one (11q13) in Hispanic Americans (N?=?3,462). For MPV (N?=?4,531), genetic variants in 3 regions were significant at p<5×10(-8), two of which were also associated with platelet count. Previously reported regions that were also significant in this study were 6p21, 6q23, 7q22, 12q24, and 19p13 for platelet count and 7q22, 17q11, and 19p13 for MPV. The most significant SNP in 1 region was also associated with ADP-induced maximal platelet aggregation in whole blood (12q24). Thus through a meta-analysis of GWAS enrolling African Americans, we have identified 5 novel regions associated with platelet count of which 3 were replicated in other ethnic groups. In addition, we also found one region associated with platelet aggregation that may play a potential role in atherothrombosis. 相似文献
25.
Warrener P Kim S Williams SM Biery M Gordon M Toniatti C Cleary MA Linsley PS Carleton M 《Apoptosis : an international journal on programmed cell death》2012,17(7):691-701
Tumor suppressor genes BRCA1 and BRCA2 function in a complex gene network that regulates homologous recombination and DNA double-strand break repair. Disruption of the BRCA-network through gene mutation, deletion, or RNAi-mediated silencing can sensitize cells to small molecule inhibitors of poly (ADP-ribose) polymerase (PARPi). Here, we demonstrate that BRCA-network disruption in the presence of PARPi leads to the selective induction and enhancement of interferon pathway and apoptotic gene expression in cultured tumor cells. In addition, we report PARPi cytotoxicity in BRCA1-deficient tumor cells is enhanced >10-fold when combined with interferon-γ. These findings establish a link between synthetic lethality of PARPi in BRCA-network disrupted cells and interferon pathway activation triggered by genetic instability. 相似文献
26.
Valerio Mori Adolfo Amici Francesca Mazzola Michele Di Stefano Laura Conforti Giulio Magni Silverio Ruggieri Nadia Raffaelli Giuseppe Orsomando 《PloS one》2014,9(11)
NAD plays essential redox and non-redox roles in cell biology. In mammals, its de novo and recycling biosynthetic pathways encompass two independent branches, the “amidated” and “deamidated” routes. Here we focused on the indispensable enzymes gating these two routes, i.e. nicotinamide mononucleotide adenylyltransferase (NMNAT), which in mammals comprises three distinct isozymes, and NAD synthetase (NADS). First, we measured the in vitro activity of the enzymes, and the levels of all their substrates and products in a number of tissues from the C57BL/6 mouse. Second, from these data, we derived in vivo estimates of enzymes''rates and quantitative contributions to NAD homeostasis. The NMNAT activity, mainly represented by nuclear NMNAT1, appears to be high and nonrate-limiting in all examined tissues, except in blood. The NADS activity, however, appears rate-limiting in lung and skeletal muscle, where its undetectable levels parallel a relative accumulation of the enzyme''s substrate NaAD (nicotinic acid adenine dinucleotide). In all tissues, the amidated NAD route was predominant, displaying highest rates in liver and kidney, and lowest in blood. In contrast, the minor deamidated route showed higher relative proportions in blood and small intestine, and higher absolute values in liver and small intestine. Such results provide the first comprehensive picture of the balance of the two alternative NAD biosynthetic routes in different mammalian tissues under physiological conditions. This fills a gap in the current knowledge of NAD biosynthesis, and provides a crucial information for the study of NAD metabolism and its role in disease. 相似文献
27.
Ion Migration and the Role of Preconditioning Cycles in the Stabilization of the J–V Characteristics of Inverted Hybrid Perovskite Solar Cells 下载免费PDF全文
28.
Dieter Blottner Najet Serradj Michele Salanova Chadi Touma Rupert Palme Mitchell Silva Jean Marie Aerts Daniel Berckmans Laurence Vico Yi Liu Alessandra Giuliani Franco Rustichelli Ranieri Cancedda Marc Jamon 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2009,179(4):519-533
Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space.
Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type
mice (C57BL/6JRj, 30 g b.wt., total n = 24) housed for 25 days in a prototypical ground-based and fully automated life support habitat device called “Mice in Space”
(MIS). Compared with control housing (individually ventilated cages) the MIS mice revealed no significant changes in soleus
muscle size and myofiber distribution (type I vs. II) and quality of bone (3-D microarchitecture and mineralisation of calvaria,
spine and femur) determined by confocal and micro-computed tomography. Corticosterone metabolism measured non-invasively (faeces)
monitored elevated adrenocortical activity at only start of the MIS cage confinement (day 1). Behavioural tests (i.e., grip
strength, rotarod, L/D box, elevated plus-maze, open field, aggressiveness) performed subsequently revealed only minor changes
in motor performance (MIS vs. controls). The MIS habitat will not, on its own, produce major effects that could confound interpretation
of data induced by microgravity exposure during spaceflight. Our results may be even more helpful in developing multidisciplinary
protocols with adequate scenarios addressing molecular to systems levels using mice of various genetic phenotypes in many
laboratories.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
29.
30.
Comparative genomic mapping between a 754 kb region flanking DREB1A in Arabidopsis thaliana and maize 总被引:3,自引:0,他引:3
Comparative mapping between model plant species for which the complete genome sequence is known and crop species has been suggested as a new strategy for the isolation of agronomically valuable genes. In this study, we tested whether comparative mapping between Arabidopsisand maize of a small region (754 kb) surrounding the DREB1A gene in Arabidopsis could lead to the identification of an orthologous region in maize containing the DREB1A homologue. The genomic sequence information available for Arabidopsis allowed for the selection of conserved, low-copy genes that were used for the identification of maize homologues in a large EST database. In total, 17 maize homologues were mapped. A second BLAST comparison of these genes to the recently completed Arabidopsis sequence revealed that 15 homologues are likely to be orthologous as the highest similarity score was obtained either with the original Arabidopsis gene or with a highly similar Arabidopsis gene localized on a duplication of the investigated region on chromosome 5. The map position of these genes showed a significant degree of orthology with the Arabidopsis region. Nevertheless, extensive duplications and rearrangements in the Arabidopsisand maize genomes as well as the evolutionary distance between Arabidopsis and maize make it unlikely that orthology and collinearity between these two species are sufficient to aid gene prediction and cloning in maize. 相似文献