全文获取类型
收费全文 | 29752篇 |
免费 | 2599篇 |
国内免费 | 228篇 |
出版年
2023年 | 247篇 |
2022年 | 286篇 |
2021年 | 645篇 |
2020年 | 463篇 |
2019年 | 503篇 |
2018年 | 931篇 |
2017年 | 767篇 |
2016年 | 979篇 |
2015年 | 1170篇 |
2014年 | 1196篇 |
2013年 | 1764篇 |
2012年 | 2243篇 |
2011年 | 2336篇 |
2010年 | 1332篇 |
2009年 | 1062篇 |
2008年 | 1777篇 |
2007年 | 1612篇 |
2006年 | 1523篇 |
2005年 | 1302篇 |
2004年 | 1352篇 |
2003年 | 1234篇 |
2002年 | 1124篇 |
2001年 | 835篇 |
2000年 | 867篇 |
1999年 | 593篇 |
1998年 | 329篇 |
1997年 | 225篇 |
1996年 | 214篇 |
1995年 | 228篇 |
1994年 | 184篇 |
1993年 | 179篇 |
1992年 | 251篇 |
1991年 | 268篇 |
1990年 | 185篇 |
1989年 | 198篇 |
1988年 | 177篇 |
1987年 | 171篇 |
1986年 | 129篇 |
1985年 | 181篇 |
1984年 | 144篇 |
1983年 | 119篇 |
1982年 | 94篇 |
1981年 | 92篇 |
1980年 | 96篇 |
1979年 | 99篇 |
1978年 | 93篇 |
1977年 | 84篇 |
1975年 | 67篇 |
1974年 | 70篇 |
1973年 | 69篇 |
排序方式: 共有10000条查询结果,搜索用时 32 毫秒
81.
The pattern of the activity of arginine decarboxylase (ADC) and omithine decarboxylase (ODC) involved in polyamine synthesis
in ripening wheat seeds was examined. The aim was to study the polyamines and the activity of the two enzymes in correlation
with the growth processes occurring in the developing wheat seeds. The results obtained showed a very different pattern of
polyamine content in the two organs of caryopsis, and that the two enzymes in the embryos have a higher activity than in the
endosperms. Moreover, while in the embryos the ADC exhibits higher activity than the ODC, in the endosperms the activity of
ODC is about similar to that of ADC. This pattern is discussed in relation to the different histological characteristics of
embryo and endosperm tissues during seed development. 相似文献
82.
The immobilization of penicillin G acylase on chitosan 总被引:3,自引:0,他引:3
83.
Permeability of human granulocytes to dimethyl sulfoxide 总被引:1,自引:0,他引:1
The permeability of the membrane of human granulocytes to the permeating solute dimethyl sulfoxide (DMSO) was studied using the Onsager form of the phenomenological equations derived from the theory of irreversible thermodynamics. Changes in cellular volume were monitored with an electronic particle counter as samples of that population were introduced into hypertonic osmotica. Temperature and concentration sensitivity analyses of the permeability coefficients were carried out. It is shown that the introduction of the Onsager formalism allows further insight into the observed transport phenomena. It was found that DMSO may affect the water permeability properties of the membrane for that population of cells. 相似文献
84.
Pathophysiological variations in the rat liver plasma membrane serine proteinase activity 总被引:2,自引:0,他引:2
The effects of fasting, diabetes, cholestasis, two-third hepatectomy and adrenalectomy on the rat liver plasma membrane serine proteinase activity were studied. Our results show a significant decrease of the enzyme activity during fasting (-50%), during experimental diabetes (-50%), in regenerating liver after partial hepatectomy (-70%) and after extrahepatic cholestasis (-70%). No modifications are noted when the rats are bilaterally adrenalectomized. These findings suggest that the enzyme activity may be linked to the level of circulating insulin, and may be regulated in physiological cellular proliferation so as to prevent undesirable protein degradation. 相似文献
85.
Effects of acetylene on hydrogenases from the sulfate reducing and methanogenic bacteria 总被引:1,自引:0,他引:1
S H He S B Woo D V DerVartanian J Le Gall H D Peck 《Biochemical and biophysical research communications》1989,161(1):127-133
The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase being 10-50 fold more sensitive than the (NiFeSe) hydrogenase. In addition the Ni(III) EPR signal (g approximately 2.3) of the "as isolated" (NiFe) hydrogenase was significantly decreased in intensity upon exposure to acetylene. 相似文献
86.
Robert A. Mueller Thomas J. McCown Ricky D. Hunt Cecilia Lundberg George R. Breese 《Cellular and molecular neurobiology》1990,10(3):327-336
1. | Rats which survived hypoglycemia by insulin, hypoxia by 10% O2, or ischemia by carotid ligation and hypotension to 40 mm Hg, evidenced no changes in cerebrospinal fluid (CSF) uridine. Animals which died soon after the above interventions or as a result of KCl-induced cardiac arrest had elevated CSF uridine concentrations. |
2. | Injection of whole blood or the soluble contents of lysed blood cells into the lateral ventricle of rats reduced CSF uridine to less than one-half normal at 24 hrs but values returned to normal 3 days later. Changes in hypoxanthine resembled those of uridine, but were less dramatic, whereas xanthine concentrations were largely unaltered. Intraventricular injection of plasma or saline did not alter CSF uridine. |
3. | It seems most likely that low CSF uridine concentrations previously reported in head injury patients may be secondary to the effects of blood cell contents in the cerebrospinal fluid, rather than responses to altered metabolism in neurons or glia cells. |
87.
Enrique Palacián Pedro J. González Manuel Piñeiro Francisco Hernández 《Molecular and cellular biochemistry》1990,97(2):101-111
Dissociation of protein-containing structures by modification of protein amino groups with dicarboxylic acid anhydrides is a mild procedure which, in some cases, offers advantages over treatment with alternative dissociating agents, such as urea, guanidine hydrochloride, detergents, high ionic strength, and extremes of pH: In addition to dissociating multimeric proteins and protein aggregates, dicarboxylic acid anhydrides are effective dissociating agents for membrane-bound proteins and nucleoprotein particles. With most dicarboxylic acid anhydrides reviewed, the introduced reagent residues can be eliminated under moderate acid conditions, which allows the purification of unmodified individual components, and the use of disassembly-reconstitution systems valuable for investigating the structural and functional roles played by the individual components of complex particles:Each reagent can be suitable for a particular purpose, depending on the required specificity of the modification and stability of the modified groups: The stability of the acylated amino groups ranges from the very stable succinylated amino groups to the very labile acylation obtained with dimethylmaleic anhydride: Between these extremes, the stability of the modified amino groups decreases stepwise in the following order: maleic, exo-cis-3,6-endoxo-4-tetrahydrophthalic, citraconic, and 3,4,5,6-tetrahydrophthalic anhydride. With respect to the selectivity of the produced modification, little or no modification of hydroxyamino acid and cysteine residues has been observed with dimethylmaleic, exo-cis-3,6-endoxo-4-tetrahydrophthalic, and 3,4,5,6-tetrahydrophthalic anhydrides: With the other reagents, the extent of modification of hydroxyamino acid residues increases in the order citraconic, maleic and succinic anhydride: Citraconic and maleic anhydrides can produce irreversible modification of cysteine residues, the reactivity of sulfhydryl groups being higher with maleic anhydride: 相似文献
88.
Mean square optical anisotropies and molar Kerr constants were calculated for homopolypeptides of the 20 natural amino acids and of several enzymes and proteins in the random-coil state. The effect of hydration was taken into account in constructing the molecular potential that gives the conformational energies as a function of the rotational angles phi and psi of the backbone and chi(1) of the side chain. The Rotational Isomeric State model was used in calculated energies, the Valence Optical Scheme and the matrix calculus technique of Flory being employed in the evaluation of the optical properties. The results are compared with calculations for the same substances that were performed without taking into account the solvent, as well as with other similar studies. The Kerr constant is confirmed as being one of the most sensitive properties of a given polypeptide to the residue class and to the sequence of those residues. 相似文献
89.
Cecilia Gotor Eloísa Pajuelo Luís C. Romero Antonio J. Márquez José M. Vega 《Archives of microbiology》1990,153(3):230-234
Polyclonal antiserum specific for ferredoxin-nitrite reductase (EC 1.7.7.1) from the green alga Chlamydomonas reinhardii recognized the nitrite reductase from other green algae, but did not cross-react with the corresponding enzyme from different cyanobacteria or higher plant leaves. An analogous situation was also found for ferredoxin-glutamate synthase (EC 1.4.7.1), using its specific antiserum. Besides, the antibodies raised against C. reinhardii ferredoxin-glutamate synthase were able to inactivate the ferredoxin-dependent activity of nitrite reductase from green algae.These results suggest that there exist similar domains in ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from green algae. In addition, both types of enzymes share common antigenic determinants, probably located at the ferredoxin-binding domain. In spite of their physicochemical resemblances, no apparent antigenic correlation exists between the corresponding enzymes from green algae and those from higher plant leaves or cyanobacteria.Abbreviations Fd
ferredoxin
- GOGAT
glutamate synthase
- MV+
reduced methyl viologen (radical cation)
- NiR
nitrite reductase
- PMSF
phenylmethylsulphonyl fluoride
- SDS
sodium dodecyl sulfate 相似文献
90.
Protoplasts were isolated from female gametophytes of Chondrus crispus (Stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria. Depending on the nature of the donor tissue (apices or whole thallus, wild or cultivated strains), yields ranged from 1.0–8.5×108 protoplasts per gram of fresh tissue. Preincubating the tissue with a potassium chelator, Kryptofix 222, enhanced protoplast yields by 30–50 %. Based on staining with fluorescein diacetate most protoplasts were viable. A few protoplasts regenerated a cell wall and divided. 相似文献