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81.
M. R. Cerbino José Cavalcante Souza Vieira C. P. Braga G. Oliveira I. F. Padilha T. M. Silva L. F. Zara N. J. SilvaJr. P. M. Padilha 《Biological trace element research》2018,181(2):216-226
Mercury is a potentially toxic element that is present in the environment of the Brazilian Amazon and is responsible for adverse health effects in humans. This study sought to assess possible protein biomarkers of mercury exposure in breast milk samples from lactating women in the Madeira and Negro Rivers in the Brazilian Amazon. The mercury content of hair samples of lactating women was determined, and the proteome of breast milk samples was obtained using two-dimensional electrophoresis after protein precipitation with acetone. Mercury measurements of protein spots obtained via protein fractionation were performed by graphite furnace atomic absorption spectrometry (GFAAS), and it was observed that mercury is linked to proteins with molecular masses in the range of 14–26 kDa. The total mercury concentration was also determined by GFAAS in unprocessed milk, lyophilized milk, and protein pellets, with the purpose of determining the mercury mass balance in relation to the concentration of this element in milk and pellets. Approximately 85 to 97% of mercury present in the lyophilized milk from samples of lactating women of the Madeira River is bound in the protein fraction. From lactating women of the Negro River, approximately 49% of the total mercury is bound in the protein fraction, and a difference of 51% is bound in the lipid fraction. 相似文献
82.
Fábio F. Cardoso Rafael J. Borges Thiago R. Dreyer Guilherme H.M. Salvador Walter L.G. Cavalcante Maeli Dal Pai Márcia Gallacci Marcos R.M. Fontes 《Biochimica et Biophysica Acta (BBA)/General Subjects》2018,1862(12):2728-2737
Background
Specific compounds found in vegetal species have been demonstrated to be efficient inhibitors of snake toxins, such as phospholipase A2-like (PLA2-like) proteins. These particular proteins, present in several species of vipers (Viperidae), induce a severe local myotoxic effect in prey and human victims, and this effect is often not efficiently neutralized by the regular serum therapy. PLA2-like proteins have been functionally and structurally studied since the early 1990s; however, a comprehensive molecular mechanism was proposed only recently.Methods
Myographic and histological techniques were used to evaluate the inhibitory effect of chicoric acid (CA) against BthTX-I myotoxin. Isothermal titration calorimetry assays were used to measure the affinity between the inhibitor and the toxin. X-ray crystallography was used to reveal details of this interaction.Results
CA prevented the blockade of indirectly evoked muscle contraction and inhibited muscle damage induced by BthTX-I. The inhibitor binds to the toxin with the highest affinity measured for a natural compound in calorimetric assays. The crystal structure and molecular dynamics simulations demonstrated that CA binds at the entrance of the hydrophobic channel of the toxin and binds to one of the clusters that participates in membrane disruption.Conclusions
CA prevents the myotoxic activity of the toxin, preventing its activation by simultaneous binding with two critical regions.General significance
CA is a potential myotoxic inhibitor to other PLA2-like proteins and a possible candidate to complement serum therapy. 相似文献83.
Vascularized outer-table calvarial bone flaps 总被引:4,自引:0,他引:4
J M Psillakis J C Grotting R Casanova D Cavalcante L O Vasconez 《Plastic and reconstructive surgery》1986,78(3):309-317
Based on an anatomic study of the vascularization of the calvarium in cadavers, a technique for the transfer of vascularized outer-table calvarial bone has been developed. The outer table of the calvarium receives numerous small perforators from its overlying periosteum. The periosteum is continuous with a distinct fascial layer overlying the temporal aponeurosis which we have termed the innominate fascia. Because of a network of anastomosing vessels from proximal branches of the superficial temporal artery and perforating branches of the deep temporal artery, the outer table of the calvarium can be carried on a pedicle which contains the temporal aponeurosis, innominate fascia, and periosteum. Thirty-seven vascularized outer-table calvarial bone flaps have been performed for a variety of craniofacial reconstructive deformities. Remarkable stability and lack of resorption have led the authors to favor this method of reconstruction particularly in poorly vascularized or previously infected recipient beds. 相似文献
84.
Chaquip D. Netto Alcides J.M. da Silva Eduardo J.S. Salustiano Thiago S. Bacelar Ingred G. Riça Moises C.M. Cavalcante Vivian M. Rumjanek Paulo R.R. Costa 《Bioorganic & medicinal chemistry》2010,18(4):1610-1616
A new pterocarpanquinone (5a) was synthesized through a palladium catalyzed oxyarylation reaction and was transformed, through electrophilic substitution reaction, into derivatives 5b–d. These compounds showed to be active against human leukemic cell lines and human lung cancer cell lines. Even multidrug resistant cells were sensitive to 5a, which presented low toxicity toward peripheral blood mononuclear cells (PBMC) cells and decreased the production of TNF-α by these cells. In the laboratory these pterocarpanquinones were reduced by sodium dithionite in the presence of thiophenol at physiological pH, as NAD(P)H quinone oxidoredutase-1 (NQO1) catalyzed two-electron reduction, and the resulting hydroquinone undergo structural rearrangements, leading to the formation of Michael acceptors, which were intercepted as adducts of thiophenol. These results suggest that these compounds could be activated by bioreduction. 相似文献
85.
Nerivânia Nunes Godeiro George Pacheco Shanlin Liu Nikolas Gioia Cipola Waldir Miron Berbel-Filho Feng Zhang Marcus Thomas P. Gilbert Bruno Cavalcante Bellini 《Zoologica scripta》2020,49(3):329-339
Seirinae is one of the most diverse subfamilies of Collembola. To date no detailed phylogeny of Seirinae has been proposed, which leads to difficulties in the understanding of evolutionary patterns regarding this taxon. The main aim of this study is to clarify the phylogenetic relationships within the Neotropical Seirinae, by generating and analysing the mitochondrial genomes of 26 terminal taxa of Entomobryidae, and one species of Paronellidae. Specifically, we first generated Illumina HiSeq 2000 shotgun sequence data from each species, then reconstructed the mitochondrial genome of each species using two methods: MitoZ and MIRA/MITOBim. Using these data, we were able to generate a well-supported phylogeny that combined all the above species as well as three publicly available mitogenomes from other species. Bayesian and maximum likelihood methods were applied using all 13 protein coding genes. In this way, monophyly for the internal groups of Seirinae was obtained based on molecular evidence for the first time, as was the potential validity of three main internal taxa of the subfamily. We furthermore validated that Tyrannoseira is a distinct lineage and propose the elevation of Lepidocyrtinus to genus. Lastly, we anticipate that these newly available mitogenomes will serve as a useful dataset for future studies on the evolution of the Collembola and Hexapoda. 相似文献
86.
87.
Effects of collagenase and elastase on the mechanical properties of lung tissue strips 总被引:2,自引:0,他引:2
Yuan Huichin; Kononov Stefanida; Cavalcante Francisco S. A.; Lutchen Kenneth R.; Ingenito Edward P.; Suki Bela 《Journal of applied physiology》2000,89(1):3-14
The dynamic stiffness (H), dampingcoefficient (G), and harmonic distortion (kd)characterizing tissue nonlinearity of lung parenchymal strips fromguinea pigs were assessed before and after treatment with elastase orcollagenase between 0.1 and 3.74 Hz. After digestion, data wereobtained both at the same mean length and at the same mean force of thestrip as before digestion. At the same mean length, G and H decreasedby ~33% after elastase and by ~47% after collagenase treatment.At the same mean force, G and H increased by ~7% after elastase andby ~25% after collagenase treatment. The kdincreased more after collagenase (40%) than after elastase (20%)treatment. These findings suggest that, after digestion, the fractionof intact fibers decreases, which, at the same mean length, leads to adecrease in moduli. At the same mean force, collagen fibers operate ata higher portion of their stress-strain curve, which results in anincrease in moduli. Also, G and H were coupled so that hysteresivity(G/H) did not change after treatments. However,kd was decoupled from elasticity and wassensitive to stretching of collagen, which may be of value in detectingstructural alterations in the connective tissue of the lung. 相似文献
88.
Simone Raposo Cotta Armando Cavalcante Franco Dias Ivanildo Evódio Marriel Fernando Dini Andreote Lucy Seldin Jan Dirk van Elsas 《Applied and environmental microbiology》2014,80(20):6437-6445
The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrogen-transforming (ammonia-oxidizing) archaea and bacteria as well as nitrogen-fixing bacteria driven by genetic modification of their maize host plants. The data show that significant changes in the abundances (revealed by quantitative PCR) of ammonia-oxidizing bacterial and archaeal communities occurred as a result of the maize host being genetically modified. In contrast, the structures of the total communities (determined by PCR-denaturing gradient gel electrophoresis) were mainly driven by factors such as soil type and season and not by plant genotype. Thus, the abundances of ammonia-oxidizing bacterial and archaeal communities but not structures of those communities were revealed to be responsive to changes in maize genotype, allowing the suggestion that community abundances should be explored as candidate bioindicators for monitoring the possible impacts of cultivation of genetically modified plants. 相似文献
89.
Betina Durovni Valeria Saraceni Susan van den Hof Anete Trajman Marcelo Cordeiro-Santos Solange Cavalcante Alexandre Menezes Frank Cobelens 《PLoS medicine》2014,11(12)
BackgroundAbundant evidence on Xpert MTB/RIF accuracy for diagnosing tuberculosis (TB) and rifampicin resistance has been produced, yet there are few data on the population benefit of its programmatic use. We assessed whether the implementation of Xpert MTB/RIF in routine conditions would (1) increase the notification rate of laboratory-confirmed pulmonary TB to the national notification system and (2) reduce the time to TB treatment initiation (primary endpoints).ConclusionsReplacing smear microscopy with Xpert MTB/RIF in Brazil increased confirmation of pulmonary TB. An additional benefit was the accurate detection of rifampicin resistance. However, no increase on overall notification rates was observed, possibly because of high rates of empirical TB treatment.
Trial registration
ClinicalTrials.gov Please see later in the article for the Editors'' Summary NCT01363765相似文献90.
Raffael AC Oliveira Ricardo VM Almeida Márcia DA Dantas Felipe N Castro Jo?o Paulo MS Lima Daniel CF Lanza 《BMC bioinformatics》2014,15(1)