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排序方式: 共有219条查询结果,搜索用时 18 毫秒
51.
Renata Damásio de Souza Milene Tavares Batista Wilson Barros Luiz Rafael Ciro Marques Cavalcante Jaime Henrique Amorim Raíza Sales Pereira Bizerra Eduardo Gimenes Martins Luís Carlos de Souza Ferreira 《PloS one》2014,9(1)
Bacillus subtilis spores have received growing attention regarding potential biotechnological applications, including the use as probiotics and in vaccine formulations. B. subtilis spores have also been shown to behave as particulate vaccine adjuvants, promoting the increase of antibody responses after co-administration with antigens either admixed or adsorbed on the spore surface. In this study, we further evaluated the immune modulatory properties of B. subtilis spores using a recombinant HIV gag p24 protein as a model antigen. The adjuvant effects of B. subtilis spores were not affected by the genetic background of the mouse lineage and did not induce significant inflammatory or deleterious effects after parenteral administration. Our results demonstrated that co-administration, but not adsorption to the spore surface, enhanced the immunogenicity of that target antigen after subcutaneous administration to BALB/c and C57BL/6 mice. Spores promoted activation of antigen presenting cells as demonstrated by the upregulation of MHC and CD40 molecules and enhanced secretion of pro-inflammatory cytokines by murine dendritic cells. In addition, in vivo studies indicated a direct role of the innate immunity on the immunomodulatory properties of B. subtilis spores, as demonstrated by the lack of adjuvant effects on MyD88 and TLR2 knockout mouse strains. 相似文献
52.
Cavalcante Deborah Garcia Mariana Aranha Maria Almeida André Merey Felipe Mendes do Amaral Crispim Bruno Barufatti Alexeia Pisani Luciana Fonseca Gustavo Braga Anna Rafaela Cavalcante de Rosso Veridiana Vera Perobelli Juliana 《Journal of applied phycology》2021,33(3):1853-1867
Journal of Applied Phycology - The present study aimed to characterize in vitro antioxidant properties of red algae (Gracilaria birdiae) powder and to investigate its potential protective and... 相似文献
53.
Cavalcante Thiago Strier Karen B. Bicca-Marques Júlio César 《International journal of primatology》2021,42(4):533-547
International Journal of Primatology - The main factors influencing feeding competition among members of diurnal primate groups are the distribution, availability, and quality of food resources.... 相似文献
54.
Sandy Sampaio Videira Michele de Cássia Pereira e Silva Péricles de Souza Galisa Armando Cavalcante Franco Dias Riitta Nissinen Vera Lúcia Baldani Divan Jan Dirk van Elsas José Ivo Baldani Joana Falcão Salles 《Plant and Soil》2013,373(1-2):737-754
Aims
Previous studies have shown that elephant grass is colonized by nitrogen-fixing bacterial species; however, these results were based on culture-dependent methods, an approach that introduces bias due to an incomplete assessment of the microbial community. In this study, we used culture-independent methods to survey the diversity of endophytes and plant-associated bacterial communities in five elephant grass genotypes used in bioenergy production.Methods
The plants of five genotypes of elephant grass were harvested from the experimental area of Embrapa Agrobiologia and divided into stem and root tissues. Total DNA and RNA were extracted from plant tissues and the bacterial communities were analyzed by DGGE and clone library of the 16S rRNA and nifH genes at both the cDNA and DNA levels.Results
Overall, the patterns based on DNA- and RNA-derived DGGE-profiles differed, especially within tissue samples. DNA-based DGGE indicated that both total bacterial and diazotrophic communities associated with roots (rhizoplane?+?endophytes) differed clearly from those obtained from stems (endophytes). These results were confirmed by the phylogenetic analyses of RNA-derived sequences of 16S rRNA (total bacteria; 586 sequences), but not for nifH (186). In fact, rarefaction analyses showed a higher diversity of diazotrophic organisms associated with stems than roots. Based on 16S rRNA sequences, the clone libraries were dominated by sequences affiliated to members of Leptotrix (12.8 %) followed by Burkholderia (9 %) and Bradyrhizobium (6.5 %), while most of the nifH clones were closely related to the genus Bradyrhizobium (26 %).Conclusions
Our results revealed an unexpectedly large diversity of metabolically active bacteria, providing new insights into the bacterial species predominantly found in association with elephant grass. Furthermore, these results can be very useful for the development of new strategies for selection of potential bacteria that effectively contribute to biological nitrogen fixation and enhance the sustainable production of elephant grass as bioenergy crop. 相似文献55.
Bruno Frederico Aguilar Calegare Augusto Azzolini Julia Ribeiro da Silva Vallim Edson Guimares Lo Turco Priscila Farias Tempaku Vanessa Cavalcante da Silva Sergio Tufik Vnia D'Almeida 《Genesis (New York, N.Y. : 2000)》2020,58(3-4)
A previous animal study by our group found that sleep deprivation during preimplantation was associated with decreased pregnancy maintenance. Given its impact on human society, we aimed in the current study to assess whether sleep deprivation affects blastocyst gene expression and/or the implantation process. For this, pregnant mice (gestational day 0 [GD 0]) were assigned into paradoxical sleep deprivation (SD, 72 hr; multiple platform method) and, a control (CT) group. Animals were euthanized on GD 3.5 and blood, uterus (embryos) and fallopian tube were collected. Then, 89% of CT presented blastocysts in the uterus versus 25% from SD group. Compared to CT, SD presented lighter relative uterus weight, increased plasma concentrations of corticosterone and testosterone, decreased concentrations of progesterone and luteinizing hormone, but no statistical differences in plasma concentrations of 17β‐estradiol and follicle stimulating hormone. There were no differences in uterus and blastocyst gene expression related to embryo implantation and development, and no alteration in blastocysts global DNA methylation. Considering this, the decreased pregnancy maintenance after sleep deprivation seems not to be associated with implantation losses or developmental problems related to the blastocysts. It is likely that complications in morula development and/or its movement through the fallopian tubes affect the pregnancy rate, since only 25% of SD females presented a blastocyst on the GD 3.5. In fact, three out of four females without blastocysts in the uterus presented morula in the fallopian tubes due to a phase delay. Additionally, we suggest that the observed hormonal changes may play a role in this outcome. 相似文献
56.
57.
de Lima Paula Monteiro Vieira José Cavalcante Souza Cavecci-Mendonça Bruna Fleuri Luciana Francisco de Lima Leite Aline Buzalaf Marília Afonso Rabelo Pezzato Luiz Edivaldo Braga Camila Pereira de Magalhães Padilha Pedro 《Biological trace element research》2020,194(1):259-272
Biological Trace Element Research - The development of metallomics techniques has allowed for metallomics analysis of biological systems, enabling a better understanding of the response mechanisms... 相似文献
58.
João Gabriel Silva Souza Martinna Bertolini Raphael Cavalcante Costa Carolina Veloso Lima 《Biofouling》2020,36(4):428-441
AbstractTitanium–zirconium (Ti–Zr) alloy has been widely used as a biomaterial for implant devices, and it is commonly treated by sandblasting followed by acid etching (SLA) to improve biological responses. Although protein adsorption is the first biological response, the effect of this SLA treatment on the proteomic profile of proteins adsorbed from saliva and blood plasma has not been tested. In this study, the proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). Streptococcus sanguinis was used to test whether the protein layer affects bacterial adhesion. SLA treatment affected the proteomic profile, showing exclusive proteins adsorbed from saliva (14) and plasma (3). However, both groups exhibited close patterns of intensity for common proteins, molecular functions and biological processes mediated by proteins. Interestingly, Ti–ZrSLA showed higher bacterial adhesion (~1.9 fold over) for the surface coated with plasma proteins. Therefore, SLA treatment of Ti–Zr alloy changed the proteomic profile, which may affect bacterial adhesion. 相似文献
59.
Litia A. Carvalho Louise C. Vitorino Roberta P.M. Guimarães Silvana Allodi Ricardo A. de Melo Reis Leny A. Cavalcante 《Biochemical and biophysical research communications》2014
We examined the effects of conditioned medium from olfactory ensheathing glia (OEGCM) on the differentiation of oligodendrocytes in mixed cultures of early postnatal hippocampi. Differentiation was judged from the numerical density (ND) of cells immunoreactive to 2′3′ cyclic nucleotide 3′phosphodiesterase (CNPase) and O4 antibodies. NDs increased according to inverted-U dose–response curves, particularly for CNPase+ cells (9-fold at optimal dilution) and these changes were blocked by inhibitors of ERK1, p38-MAPK, and PI3K. Our results raise the possibility that OEG secreted factor(s) may counteract demyelination induced by trauma, neurodegenerative diseases, and advanced age, and should stimulate novel methods to deliver these factors and/or potentiating chemicals. 相似文献
60.
Ricardo de Souza Cavalcante Tatiane Fernanda Sylvestre Adriele Dandara Levorato Lídia Rachel de Carvalho Rinaldo Poncio Mendes 《PLoS neglected tropical diseases》2014,8(4)