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451.
Kripa K. Krishna V. Bhumika Monson Thomas P. Anil Kumar T. N. R. Srinivas 《Antonie van Leeuwenhoek》2013,103(5):1015-1021
A novel Gram-negative, spiral shaped, motile bacterium, designated strain NIO-S6T, was isolated from a sediment sample collected from Off-shore Rameswaram, Tamilnadu, India. Strain NIO-S6T was found to be positive for oxidase, DNase and lysine decarboxylase activities and negative for catalase, gelatinase, lipase, ornithine decarboxylase, nitrate reductase, aesculinase, amylase and urease activities. The fatty acids were determined to be dominated by C10:0 3OH, C16:0, C16:1 and C18:1. Strain NIO-S6T contains Q-8 as the major respiratory quinone. The DNA G+C content of the strain NIO-S6T was determined to be 49.5 ± 0.6 mol %. Phylogenetic analysis based on 16S rRNA gene sequence of strain NIO-S6T indicated Oceanospirillum linum and Oceanospirillum maris of the family Oceanospirillaceae (phylum Proteobacteria) are the closest related species with sequence similarities of 98.4 and 97.8 % respectively. Other members of the family showed sequence similarities <96.4 %. However, DNA–DNA hybridization with Oceanospirillum linum LMG 5214T and Oceanospirillum maris LMG 5213T showed a relatedness of 31.5 and 46.9 % with respect to strain NIO-S6T. Based on the phenotypic characteristics and on phylogenetic inference, strain NIO-S6T is proposed as a novel species of the genus Oceanospirillum as Oceanospirillum nioense sp. nov. and the type strain is NIO-S6T (=MTCC 11154T = KCTC 32008T). 相似文献
452.
A drip irrigation delivery system was used to infest field sites with the plant-parasitic root-knot nematodes, Meloidogyne incognita. Juvenile or egg inocula passed through the system without blockage of emitters or harm to the nematodes. Field sites so infested were available for experimentation. Delivery of approximately 5 x 10⁴ to 10⁵ juveniles or 10⁵ to 3 x 10⁵ eggs per emitter through the drip system resulted in heavy root galling of tomatoes planted next to the drip emitters. Nematodes feeding on bacteria (Acrobeloides sp.) and on fungi (Deladenus durus) also were successfully applied through the drip system. This method has potential for uniformly infesting experimental sites with plant-parasitic or entomogenous nematodes and for manipulation of nematode community structure for soil ecological studies. 相似文献
453.
Plasmid DNA in colorless filamentous gliding bacteria 总被引:2,自引:0,他引:2