全文获取类型
收费全文 | 11811篇 |
免费 | 962篇 |
国内免费 | 11篇 |
出版年
2023年 | 56篇 |
2022年 | 97篇 |
2021年 | 238篇 |
2020年 | 106篇 |
2019年 | 153篇 |
2018年 | 214篇 |
2017年 | 164篇 |
2016年 | 288篇 |
2015年 | 507篇 |
2014年 | 591篇 |
2013年 | 795篇 |
2012年 | 892篇 |
2011年 | 923篇 |
2010年 | 577篇 |
2009年 | 499篇 |
2008年 | 772篇 |
2007年 | 783篇 |
2006年 | 708篇 |
2005年 | 682篇 |
2004年 | 677篇 |
2003年 | 638篇 |
2002年 | 568篇 |
2001年 | 120篇 |
2000年 | 85篇 |
1999年 | 131篇 |
1998年 | 173篇 |
1997年 | 108篇 |
1996年 | 89篇 |
1995年 | 95篇 |
1994年 | 103篇 |
1993年 | 93篇 |
1992年 | 78篇 |
1991年 | 54篇 |
1990年 | 59篇 |
1989年 | 54篇 |
1988年 | 56篇 |
1987年 | 38篇 |
1986年 | 34篇 |
1985年 | 37篇 |
1984年 | 45篇 |
1983年 | 35篇 |
1982年 | 35篇 |
1981年 | 40篇 |
1980年 | 45篇 |
1979年 | 37篇 |
1978年 | 23篇 |
1977年 | 25篇 |
1976年 | 20篇 |
1974年 | 20篇 |
1973年 | 22篇 |
排序方式: 共有10000条查询结果,搜索用时 93 毫秒
231.
Olivier Maurin Artemis Anest Sidonie Bellot Edward Biffin Grace Brewer Tristan Charles-Dominique Robyn S. Cowan Steven Dodsworth Niroshini Epitawalage Berta Gallego Augusto Giaretta Renato Goldenberg Deise J.P. Gonçalves Shirley Graham Peter Hoch Fiorella Mazine Yee Wen Low Catherine McGinnie Fabián A. Michelangeli Sarah Morris Darin S. Penneys Oscar Alejandro Pérez Escobar Yohan Pillon Lisa Pokorny Gustavo Shimizu Vanessa G. Staggemeier Andrew H. Thornhill Kyle W. Tomlinson Ian M. Turner Thais Vasconcelos Peter G. Wilson Alexandre R. Zuntini William J. Baker Félix Forest Eve Lucas 《American journal of botany》2021,108(7):1087-1111
232.
233.
234.
235.
Yulia Mostovoy Feyza Yilmaz Stephen K Chow Catherine Chu Chin Lin Elizabeth A Geiger Naomi J L Meeks Kathryn C Chatfield Curtis R Coughlin II Urvashi Surti Pui-Yan Kwok Tamim H Shaikh 《Genetics》2021,217(2)
Segmental duplications (SDs) are a class of long, repetitive DNA elements whose paralogs share a high level of sequence similarity with each other. SDs mediate chromosomal rearrangements that lead to structural variation in the general population as well as genomic disorders associated with multiple congenital anomalies, including the 7q11.23 (Williams–Beuren Syndrome, WBS), 15q13.3, and 16p12.2 microdeletion syndromes. Population-level characterization of SDs has generally been lacking because most techniques used for analyzing these complex regions are both labor and cost intensive. In this study, we have used a high-throughput technique to genotype complex structural variation with a single molecule, long-range optical mapping approach. We characterized SDs and identified novel structural variants (SVs) at 7q11.23, 15q13.3, and 16p12.2 using optical mapping data from 154 phenotypically normal individuals from 26 populations comprising five super-populations. We detected several novel SVs for each locus, some of which had significantly different prevalence between populations. Additionally, we localized the microdeletion breakpoints to specific paralogous duplicons located within complex SDs in two patients with WBS, one patient with 15q13.3, and one patient with 16p12.2 microdeletion syndromes. The population-level data presented here highlights the extreme diversity of large and complex SVs within SD-containing regions. The approach we outline will greatly facilitate the investigation of the role of inter-SD structural variation as a driver of chromosomal rearrangements and genomic disorders. 相似文献
236.
Jrme Goudeau Catherine S Sharp Jonathan Paw Laura Savy Manuel D Leonetti Andrew G York Dustin L Updike Cynthia Kenyon Maria Ingaramo 《Genetics》2021,217(4)
We create and share a new red fluorophore, along with a set of strains, reagents and protocols, to make it faster and easier to label endogenous Caenorhabditis elegans proteins with fluorescent tags. CRISPR-mediated fluorescent labeling of C. elegans proteins is an invaluable tool, but it is much more difficult to insert fluorophore-size DNA segments than it is to make small gene edits. In principle, high-affinity asymmetrically split fluorescent proteins solve this problem in C. elegans: the small fragment can quickly and easily be fused to almost any protein of interest, and can be detected wherever the large fragment is expressed and complemented. However, there is currently only one available strain stably expressing the large fragment of a split fluorescent protein, restricting this solution to a single tissue (the germline) in the highly autofluorescent green channel. No available C. elegans lines express unbound large fragments of split red fluorescent proteins, and even state-of-the-art split red fluorescent proteins are dim compared to the canonical split-sfGFP protein. In this study, we engineer a bright, high-affinity new split red fluorophore, split-wrmScarlet. We generate transgenic C. elegans lines to allow easy single-color labeling in muscle or germline cells and dual-color labeling in somatic cells. We also describe a novel expression strategy for the germline, where traditional expression strategies struggle. We validate these strains by targeting split-wrmScarlet to several genes whose products label distinct organelles, and we provide a protocol for easy, cloning-free CRISPR/Cas9 editing. As the collection of split-FP strains for labeling in different tissues or organelles expands, we will post updates at doi.org/10.5281/zenodo.3993663 相似文献
237.
238.
In times where many people have suffered loss and others of us are dealing with stress, disruption, and fear, there is a lot of comfort to be taken in reading. If we are not able to meet up and discuss our work in person, exploring published studies provides some succor, even without the cheese, wine, and other traditions of our usual get-togethers. Fortunately, recent months have seen many high-quality papers around the topic of glycosaminoglycans. I can only pick up on a very few here, those that I have particularly enjoyed, but the following collection of reviews will also be a treat and hopefully tide us over until our research community can regroup: 相似文献
239.
Michael A.P. Bloomfield Tinya Chang Maximillian J. Woodl Laura M. Lyons Zhen Cheng Clarissa Bauer‐Staeb Catherine Hobbs Sophie Bracke Helen Kennerley Louise Isham Chris Brewin Jo Billings Talya Greene Glyn Lewis 《World psychiatry》2021,20(1):107-123
Experiencing psychological trauma during childhood and/or adolescence is associated with an increased risk of psychosis in adulthood. However, we lack a clear knowledge of how developmental trauma induces vulnerability to psychotic symptoms. Understanding the psychological processes involved in this association is crucial to the development of preventive interventions and improved treatments. We sought to systematically review the literature and combine findings using meta‐analytic techniques to establish the potential roles of psychological processes in the associations between developmental trauma and specific psychotic experiences (i.e., hallucinations, delusions and paranoia). Twenty‐two studies met our inclusion criteria. We found mediating roles of dissociation, emotional dysregulation and post‐traumatic stress disorder (PTSD) symptoms (avoidance, numbing and hyperarousal) between developmental trauma and hallucinations. There was also evidence of a mediating role of negative schemata, i.e. mental constructs of meanings, between developmental trauma and delusions as well as paranoia. Many studies to date have been of poor quality, and the field is limited by mostly cross‐sectional research. Our findings suggest that there may be distinct psychological pathways from developmental trauma to psychotic phenomena in adulthood. Clinicians should carefully ask people with psychosis about their history of developmental trauma, and screen patients with such a history for dissociation, emotional dysregulation and PTSD symptoms. Well conducted research with prospective designs, including neurocognitive assessment, is required in order to fully understand the biopsychosocial mechanisms underlying the association between developmental trauma and psychosis. 相似文献
240.
McCulloch Lindsay A. Kropp Heather Kholodov Alexander Cardels Catherine L. Natali Susan M. Loranty Michael M. 《Ecosystems》2021,24(6):1332-1347
Ecosystems - Carbon cycle perturbations in high-latitude ecosystems associated with rapid warming can have implications for the global climate. Belowground biomass is an important component of the... 相似文献