全文获取类型
收费全文 | 360篇 |
免费 | 31篇 |
出版年
2021年 | 3篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 15篇 |
2013年 | 15篇 |
2012年 | 23篇 |
2011年 | 16篇 |
2010年 | 10篇 |
2009年 | 13篇 |
2008年 | 10篇 |
2007年 | 20篇 |
2006年 | 13篇 |
2005年 | 10篇 |
2004年 | 13篇 |
2003年 | 10篇 |
2002年 | 5篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 12篇 |
1998年 | 7篇 |
1997年 | 10篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 11篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 5篇 |
1979年 | 7篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1974年 | 4篇 |
1973年 | 2篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1928年 | 2篇 |
1917年 | 3篇 |
1916年 | 3篇 |
1915年 | 3篇 |
排序方式: 共有391条查询结果,搜索用时 46 毫秒
151.
Paola Goldoni Lorena Cattani Stefania Carrara Maddalena Castellani Pastoris Laura Sinibaldi Nicola Orsi 《Microbiology and immunology》1998,42(4):271-279
A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila. Only cytochalasin A and 2-deoxy-d -glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect. 相似文献
152.
Lakshmi Narayan Shanmugham Maria Luisa Castellani Vincenzo Salini Katia Falasca Jacopo Vecchiet Pio Conti Claudia Petrarca 《Theoretical biology forum》2006,99(2):227-249
Protein-carbohydrate interactions are used for intercellular communication. Mammalian cells are known to bear a variety of glycoconjugates. Lectins, first discovered in plants, are proteins which can specifically bind carbohydrates. Given the high affinity of plant lectins for carbohydrates, they have always been important as molecular tools in the identification, purification and stimulation of specific glycoproteins on human cells. Lectins have provided important clues to the repertoire of carbohydrate structures in animal cells. The discovery of plant lectins gave a great impulse to modern glycobiology. They represent important biochemical reagents for numerous applications in the biomedical field and in research. Sequence determinations and structural characterization helped to understand the mechanism of action in many biologic systems. Plant lectins have been fundamental in human immunological studies because some of them are mitogenic/activating to lymphocytes. Understanding the molecular basis of lectin-carbohydrate interactions and of the intracellular signalling evoked holds promise for the design of novel drugs for the treatment of infectious, inflammatory and malignant diseases. It may also be of help for the structural and functional investigation of glycoconjugates and their changes during physiological and pathological processes. 相似文献
153.
154.
Transformed human cells release different fibronectin variants than do normal cells 总被引:14,自引:7,他引:7
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
P Castellani A Siri C Rosellini E Infusini L Borsi L Zardi 《The Journal of cell biology》1986,103(5):1671-1677
Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65% of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28% of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma. 相似文献
155.
Marina Veronesi Francesca Giacomina Elisa Romeo Beatrice Castellani Giuliana Ottonello Chiara Lambruschini Gianpiero Garau Rita Scarpelli Tiziano Bandiera Daniele Piomelli Claudio Dalvit 《Analytical biochemistry》2016
Nuclear magnetic resonance (NMR)-based screening has been recognized as a powerful approach for the identification and characterization of molecules interacting with pharmaceutical targets. Indeed, several NMR methods have been developed and successfully applied to many drug discovery projects. Whereas most of these approaches have targeted isolated biomolecular receptors, very few cases are reported with the screening performed in intact cells and cell extracts. Here we report the first successful application of the fluorine NMR-based assay n-FABS (n-fluorine atoms for biochemical screening) in living mammalian cells expressing the membrane protein fatty acid amide hydrolase (FAAH). This method allows the identification of both weak and potent inhibitors and the measurement of their potency in a physiological environment. 相似文献
156.
157.
158.
S Guizzardi L Leonardi M Franchi P P Castellani R Riva 《Bollettino della Società italiana di biologia sperimentale》1983,59(12):1767-1772
The collagen fibers observed in calcified valves appear randomly distributed without any preferential arrangement. In these areas also the hydroxylapatite crystals do not show any orientation. It is suggested that the disorder of the organic phase is a factor favouring the deposition of the calcium crystals. 相似文献
159.
160.
Abstract Using direct fluorescent antibody (DFA) staining technique, Legionella pneumophila SG 1, 3 and 5 was evident in water samples collected from different localities of central Italian regions, Marche and Abruzzi; L. pneumophila SG 1 and 3 was also detected in aquatic stages of arthropods living in the Legionella -positive waters. Diptera, Coleoptera, Collembola and Isopoda were found to be positive for legionellas by DFA. Diptera, the most common in the waters surveyed, were represented by Chironomidae and Culicidae families, the latter being larval and pupal stages of genus Anopheles and Culex . Mosquito adults, emerged in laboratory from pupae collected in one sample of positive water, were also positive. The findings that aquatic arthropods harbor legionellas and whether they could be involved in the maintenance and dissemination of legionellas in nature are discussed. 相似文献