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61.
Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova. Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidity show optimal bioluminescence emission at 25 degrees C in the presence of 1.0% sugar cane molasses, 0.10% yeast extract and pH 6.0 (nonbuffered). Temperature and pH are the most important factors for both mycelial growth and bioluminescence. 相似文献
62.
Tadeu Siqueira Luis Mauricio Bini Marcus Vinicius Cianciaruso Fabio Oliveira Roque Susana Trivinho-Strixino 《Hydrobiologia》2009,620(1):163-172
In situ measurements of both community metabolism (primary production and respiration) and PAM fluorometry were conducted
during emersion on intertidal sediments in the Mont Saint-Michel Bay, in areas where oysters and mussels were cultivated.
Results highlighted a low benthic metabolism compared to other intertidal areas previously investigated with the same methods.
Comparisons between gross community primary production and relative electron transport rates confirmed this statement. More
specifically, primary productivity remained very low all over the year, whereas the associated microalgal biomass was estimated
to be high. We suggest that the microphytobenthic community studied was characterized by a self-limitation of its primary
productivity by its own biomass, as previously shown in Marennes-Oléron Bay for example. The almost permanent high biomass
would represent a limiting factor for micromigration processes within the first millimetres of the sediment. This could be
explained by very low resuspension processes occurring in the western part of the bay, enhanced by the occurrence of numerous
aquaculture structures that could decrease tidal currents in the benthic boundary layer.
Handling editor: N. Desroy 相似文献
63.
Aline C. Intorne Marcos Vinicius V. de Oliveira Mariana L. Lima Juliana F. da Silva Fábio L. Olivares Gonçalo Apolinário de Souza Filho 《Archives of microbiology》2009,191(5):477-483
Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance.
The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones,
action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus
and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective
for phosphorous [Ca5(PO4)3OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently,
allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate
that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
A. C. Intorne and M. V. V. de Oliveira contributed equally to this work. 相似文献
64.
Thaís Helena Gasparoto Narciso Almeida Vieira Vinicius Carvalho Porto Ana Paula Campanelli Vanessa Soares Lara 《Immunity & ageing : I & A》2009,6(1):3
Background
Ageing leads to a decline in the function of the immune system, increasing the body's susceptibility to infections through the impairment of T-cells, macrophages, neutrophils and dendritic cells Denture stomatitis is a primary oral disease affecting elderly denture wearers. The major etiologic factor involved in this pathology is the infection by Candida albicans, an opportunistic pathogen that causes local and disseminated diseases in immunosuppressed humans. Neutrophils play a critical role in the immune response against C. albicans and are continually present in the salivary fluid and in the blood. The aim of this study was to determine ageing-related changes in salivary and blood neutrophils and their potential implications in Candida-related denture stomatitis. 相似文献65.
Polyclad flatworms have a troubled classification history, with two contradicting systems in use. They both rely on a ventral adhesive structure to define the suborders Acotylea and Cotylea, but superfamilies were defined according to eyespot arrangement (Prudhoe’s system) or prostatic vesicle characters (Faubel’s system). Molecular data available cover a very limited part of the known polyclad family diversity and have not allowed testing morphology-based classification systems on Polycladida yet. We thus sampled a suitable marker, partial 28S ribosomal DNA (rDNA), from Polycladida (19 families and 32 genera), generating 136 new sequences and the first comprehensive genetic dataset on polyclads. Our maximum likelihood (ML) analyses recovered Polycladida, but the traditional suborders were not monophyletic, as the supposedly acotyleans Cestoplana and Theama were nested within Cotylea; we suggest that these genera should be included in Cotylea. The partial 28S rDNA trees were generally well supported and robust but in conflict with both Faubel’s and Prudhoe’s superfamilies. Therefore, we compiled morphological and anatomical characters for all taxa used and examined their distribution on our molecular tree. Combining morphological and molecular evidence, we redefined polyclad superfamilies. Acotylea contain tentaculated and atentaculated groups and is now divided in three superfamilies. The suborder Cotylea can be divided in five superfamilies. In general, there is a trait of anteriorization of sensory structures, from the plesiomorphic acotylean body plan to the cotylean gross morphology. Traditionally used characters, such as prostatic vesicle, eyespot distribution, and type of pharynx, are all homoplastic and likely have misled polyclad systematics so far. 相似文献
66.
Maldaner G Marangon P Ilha V Caro MS Burrow RA Dalcol II Morel AF 《Phytochemistry》2011,72(8):804-809
Scutianene E (1), 3,4,28-tris-epi-scutiaene E (2), 28-epi-scutianene E (3) and scutianene L (4), four neutral cyclopeptide alkaloids, were isolated from Scutia buxifolia Reiss, together with four known cyclopeptide alkaloids, scutianines B, C, D and E. Scutianenes 1-3 are diastereoisomeric compounds, with 3-hydroxyleucine as a β-hydroxy amino acid unit, which is connected to the styryl fragment via an ether bridge, β-phenylserine, as a common ring-bonded amino acid residue. Attached to the amino group of β-hydroxyamino acid is a side chain [trans-CH = CH-Ph]. The structures of the peptides were elucidated by means of spectroscopic analysis, including extensive 2D NMR studies. The stereochemistry for the diastereomeric 3,4,28-tris-epi-scutiaene E and 28-epi-scutianene E was confirmed by X-ray diffraction analysis of their O-acetyl derivatives. 相似文献
67.
Da Silva AS Fanfa VR Otto MA Gressler LT Tavares KC Lazzarotto CR Tonin AA Miletti LC Duarte MM Monteiro SG 《The Korean journal of parasitology》2011,49(4):427-430
The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a strain of T. evansi and human plasma (plasmas 1, 2, and 3) from 3 adult males clinically healthy were used. In vivo test used 50 mice divided in 5 groups (A to E) with 10 animals in each group. Animals of groups B to E were infected, and then treated with 0.2 ml of human plasma in the following outline: negative control (A), positive control (B), treatment with plasma 1 (C), treatment with plasma 2 (D), and treatment with plasma 3 (E). Mice treated with human plasma showed an increase in longevity of 40.9 ± 0.3 (C), 20 ± 9.0 (D) and 35.6 ± 9.3 (E) days compared to the control group (B) which was 4.3 ± 0.5 days. The number of surviving mice and free of the parasite (blood smear and PCR negative) at the end of the experiment was 90%, 0%, and 60% for groups C, D, and E, respectively. The quantification of APOL1 was performed due to the large difference in the treatments that differed in the source plasma. In plasmas 1, 2, and 3 was detected the concentration of 194, 99, and 115 mg/dl of APOL1, respectively. However, we believe that this difference in the treatment efficiency is related to the level of APOL1 in plasmas. 相似文献
68.
Matheus R. de Mendonça Leandro G. Rizzi Vinicius Contessoto Vitor B. P. Leite Nelson A. Alves 《Proteins》2014,82(1):119-129
A number of studies have demonstrated that simple elastic network models can reproduce experimental B‐factors, providing insights into the structure–function properties of proteins. Here, we report a study on how to improve an elastic network model and explore its performance by predicting the experimental B‐factors. Elastic network models are built on the experimental coordinates, and they only take the pairs of atoms within a given cutoff distance rc into account. These models describe the interactions by elastic springs with the same force constant. We have developed a method based on numerical simulations with a simple coarse‐grained force field, to attribute weights to these spring constants. This method considers the time that two atoms remain connected in the network during partial unfolding, establishing a means of measuring the strength of each link. We examined two different coarse‐grained force fields and explored the computation of these weights by unfolding the native structures. Proteins 2014; 82:119–129. © 2013 Wiley Periodicals, Inc. 相似文献
69.
Purification,characterization and partial sequence of a pro‐inflammatory lectin from seeds of Canavalia oxyphylla Standl. & L. O. Williams
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Mayara Q. Santiago Cintia C. F. Leitão Francisco N. Pereira‐Junior Vanir R. Pinto‐Junior Vinicius J. S. Osterne Claudia F. Lossio João B. Cajazeiras Helton C. Silva Francisco V. S. Arruda Livia P. Pereira Ana M. S. Assreuy Kyria S. Nascimento Celso S. Nagano Benildo S. Cavada 《Journal of molecular recognition : JMR》2014,27(3):117-123
Recent studies have shown that lectins are promising tools for use in various biotechnological processes, as well as studies of various pathological mechanisms, isolation, and characterization of glycoconjugates and understanding the mechanisms underlying pathological mechanisms conditions, including the inflammatory response. This study aimed to purify, characterize physicochemically, and predict the biological activity of Canavalia oxyphylla lectin (CoxyL) in vitro and in vivo. CoxyL was purified by a single‐step affinity chromatography in Sephadex® G‐50 column. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the pure lectin consists of a major band of 30 kDa (α‐chain) and two minor components (β‐chain and γ‐chain) of 16 and 13 kDa, respectively. These data were further confirmed by electrospray ionization mass spectrometry, suggesting that CoxyL is a typical ConA‐like lectin. In comparison with the average molecular mass of α‐chain, the partial amino acid sequence obtained corresponds to approximately 45% of the total CoxyL sequence. CoxyL presented hemagglutinating activity that was specifically inhibited by monosaccharides (D‐glucose, D‐mannose, and α‐methyl‐D‐mannoside) and glycoproteins (ovalbumin and fetuin). Moreover, CoxyL was shown to be thermostable, exhibiting full hemagglutinating activity up to 60°C, and it was pH‐sensitive for 1 h, exhibiting maximal activity at pH 7.0. CoxyL caused toxicity to Artemia nauplii and induced paw edema in rats. This biological activity highlights the importance of lectins as important tools to better understand the mechanisms underlying inflammatory responses. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
70.
Julián E. Muñoz Vinicius D. Luft Juliana Amorim Adriana Magalhães Luciana Thomaz Joshua D. Nosanchuk Luiz R. Travassos Carlos P. Taborda 《Mycopathologia》2014,178(3-4):177-188
Paracoccidioidomycosis is a systemic granulomatous disease caused by Paracoccidioides spp. A peptide from the major diagnostic antigen gp43, named P10, induces a T-CD4+ helper-1 immune response in mice and protects against intratracheal challenge with virulent P. brasiliensis. Previously, we evaluated the efficacy of the P10 peptide alone or combined with antifungal drugs in mice immunosuppressed and infected with virulent isolate of P. brasiliensis. In the present work, our data suggest that P10 immunization leads to an effective cellular immune response associated with an enhanced T cell proliferative response. P10-stimulated splenocytes increased nitric oxide (NO) production and induced high levels of IFN-γ, IL-1β and IL-12. Furthermore, significantly increased concentrations of pro-inflammatory cytokines were also observed in lung homogenates of immunized mice. P10 immunization was followed by minimal fibrosis in response to infection. Combined with antifungal drugs, P10 immunization most significantly improved survival of anergic infected mice. Administration of either itraconazole or sulfamethoxazole/trimethoprim together with P10 immunization resulted in 100 % survival up to 200 days post-infection, whereas untreated mice died within 80 days. Hence, our data show that P10 immunization promotes a strong specific immune response even in immunocompromised hosts and thus P10 treatment represents a powerful adjuvant therapy to chemotherapy. 相似文献