全文获取类型
收费全文 | 289篇 |
免费 | 20篇 |
国内免费 | 32篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 4篇 |
2020年 | 6篇 |
2019年 | 4篇 |
2017年 | 4篇 |
2016年 | 3篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 5篇 |
2012年 | 18篇 |
2011年 | 24篇 |
2010年 | 8篇 |
2009年 | 12篇 |
2008年 | 9篇 |
2007年 | 9篇 |
2006年 | 9篇 |
2005年 | 6篇 |
2004年 | 7篇 |
2003年 | 4篇 |
2002年 | 9篇 |
2000年 | 4篇 |
1999年 | 7篇 |
1998年 | 4篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 10篇 |
1983年 | 7篇 |
1982年 | 3篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 6篇 |
1971年 | 7篇 |
1970年 | 5篇 |
1969年 | 5篇 |
1968年 | 2篇 |
1967年 | 5篇 |
1966年 | 7篇 |
排序方式: 共有341条查询结果,搜索用时 15 毫秒
11.
12.
13.
14.
15.
H. Caspers 《International Review of Hydrobiology》1984,69(1):40-40
16.
17.
Synthesis, processing and export of cytoplasmic endo-beta-1,4-xylanase from barley aleurone during germination 总被引:1,自引:0,他引:1
Caspers MP Lok F Sinjorgo KM van Zeijl MJ Nielsen KA Cameron-Mills V 《The Plant journal : for cell and molecular biology》2001,26(2):191-204
We have identified the major endo-beta-1,4-xylanase (XYN-1) in the aleurone of germinating barley grain, and show that it is expressed as a precursor of Mr 61 500 with both N- and C-terminal propeptides. XYN-1 is synthesized as an inactive enzyme in the cytoplasm, and only becomes active at a late stage of germination when the aleurone ceases to secrete hydrolases. A series of processing steps, mediated in part by aleurone cysteine endoproteases, yields a mature active enzyme of Mr 34 000. Processing and extracellular release of the mature enzyme coincide with the programmed cell death (PCD)-regulated disintegration of aleurone cells. We discuss the significance of delayed aleurone cell-wall degradation by endoxylanases in relation to the secretory capacity of the aleurone, and propose a novel role for aleurone PCD in facilitating the export of hydrolases. 相似文献
18.
van der Veen BA Uitdehaag JC Dijkstra BW Dijkhuizen L 《Biochimica et biophysica acta》2000,1543(2):336-360
19.
von Ahsen O Lim JH Caspers P Martin F Schönfeld HJ Rassow J Pfanner N 《Journal of molecular biology》2000,297(3):809-818
Cyclophilins accelerate slow protein folding reactions in vitro by catalyzing the cis/trans isomerization of peptidyl-prolyl bonds. Cyclophilins were reported to be involved in a variety of cellular functions, including the promotion of protein folding by use of the substrate mouse dihydrofolate reductase (DHFR). The interaction of cyclophilin with DHFR has only been studied under limited conditions so far, not taking into account that native DHFR exists in equilibrium with a non-native late-folding intermediate. Here we report a systematic analysis of catalysis of DHFR folding by cyclophilins. The specific ligand methotrexate traps DHFR in its native state, permitting a specific analysis of the action of cyclophilin on both denatured DHFR with non-native prolyl bonds and denatured DHFR with all-native prolyl bonds. Cyclophilins from yeast and Neurospora crassa as well as the related prolyl isomerase b from Escherichia coli promote the folding of different forms of DHFR to the enzymatically active form, demonstrating the generality of cyclophilin-catalyzed folding of DHFR. The slow equilibrium between the late-folding intermediate and native DHFR suggests that prolyl isomerization may be required for this final phase of conversion to native DHFR. However, by reversible trapping of the intermediate, we analyze the slow interconversion between native and late-folding conformations in the backward and forward reactions and show a complete independence of cyclophilin. We conclude that cyclophilin catalyzes folding of DHFR, but surprisingly not in the last slow folding step. 相似文献
20.
Lia Judice M. Relvas Maya Makhoul Remi Dewispelaere Laure Caspers Didier Communi Jean-Marie Boeynaems Bernard Robaye Catherine Bruyns Fran?ois Willermain 《PloS one》2015,10(2)
We aimed to study the role of the nucleotide receptor P2Y2R in the development of experimental autoimmune uveitis (EAU). EAU was induced in P2Y2+/+ and P2Y2-/- mice by immunization with IRBP peptide or by adoptive transfer of in vitro restimulated semi-purified IRBP-specific enriched T lymphocytes from spleens and lymph nodes isolated from native C57Bl/6 or P2Y2+/+ and P2Y2-/- immunized mice. Clinical and histological scores were used to grade disease severity. Splenocytes and lymph node cell phenotypes were analyzed using flow cytometry. Semi-purified lymphocytes and MACS-purified CD4+ T lymphocytes from P2Y2+/+ and P2Y2-/- immunized mice were tested for proliferation and cytokine secretion. Our data show that clinical and histological scores were significantly decreased in IRBP-immunized P2Y2-/- mice as in P2Y2-/- mice adoptively transfered with enriched T lymphocytes from C57Bl/6 IRBP-immunized mice. In parallel, naïve C57Bl/6 mice adoptively transferred with T lymphocytes from P2Y2-/- IRBP-immunized mice also showed significantly less disease. No differences in term of spleen and lymph node cell recruitment or phenotype appeared between P2Y2-/- and P2Y2+/+ immunized mice. However, once restimulated in vitro with IRBP, P2Y2-/- T cells proliferate less and secrete less cytokines than the P2Y2+/+ one. We further found that antigen-presenting cells of P2Y2-/- immunized mice were responsible for this proliferation defect. Together our data show that P2Y2-/- mice are less susceptible to mount an autoimmune response against IRBP. Those results are in accordance with the danger model, which makes a link between autoreactive lymphocyte activation, cell migration and the release of danger signals such as extracellular nucleotides. 相似文献