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91.
Association of Rab25 and Rab11a with the Apical Recycling System of Polarized Madin–Darby Canine Kidney Cells 下载免费PDF全文
James E. Casanova Xiaoye Wang Ravindra Kumar Sheela G. Bhartur Jennifer Navarre Julie E. Woodrum Yoram Altschuler Greg S. Ray James R. Goldenring 《Molecular biology of the cell》1999,10(1):47-61
Recent evidence suggests that apical and basolateral endocytic pathways in epithelia converge in an apically located, pericentriolar endosomal compartment termed the apical recycling endosome. In this compartment, apically and basolaterally internalized membrane constituents are thought to be sorted for recycling back to their site of origin or for transcytosis to the opposite plasma membrane domain. We report here that in the epithelial cell line Madin–Darby Canine Kidney (MDCK), antibodies to Rab11a label an apical pericentriolar endosomal compartment that is dependent on intact microtubules for its integrity. Furthermore, this compartment is accessible to a membrane-bound marker (dimeric immunoglobulin A [IgA]) internalized from either the apical or basolateral pole, functionally defining it as the apical recycling endosome. We have also examined the role of a closely related epithelial-specific Rab, Rab25, in the regulation of membrane recycling and transcytosis in MDCK cells. When cDNA encoding Rab25 was transfected into MDCK cells, the protein colocalized with Rab11a in subapical vesicles. Rab25 transfection also altered the distribution of Rab11a, causing the coalescence of immunoreactivity into multiple denser vesicular structures not associated with the centrosome. Nevertheless, nocodazole still dispersed these vesicles, and dimeric IgA internalized from either the apical or basolateral membrane was detected in endosomes labeled with antibodies to both Rab11a and Rab25. Overexpression of Rab25 decreased the rate of IgA transcytosis and of apical, but not basolateral, recycling of internalized ligand. Conversely, expression of the dominant-negative Rab25T26N did not alter either apical recycling or transcytosis. These results indicate that both Rab11a and Rab25 associate with the apical recycling system of epithelial cells and suggest that Rab25 may selectively regulate the apical recycling and/or transcytotic pathways. 相似文献
92.
Armstrong Andrew M. Sobsey Mark D. Casanova Lisa M. 《Applied microbiology and biotechnology》2017,101(18):6891-6897
Applied Microbiology and Biotechnology - Households that lack piped water supply are often forced to meet water needs by storing in the home, leaving water vulnerable to contamination by viruses.... 相似文献
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In this article, the lithic reduction systems from Middle Paleolithic levels at Roca dels Bous and Tragó are presented. These two sites are located in the South-eastern Pyrenees in Catalunya (Spain) and yield Mousterian levels which are attributed to MIS 3 and MIS 5. At the two studied sites, there is coexistence between expedient knapping systems and more complex techniques such as the Levallois method. Furthermore, cores are heavily exhausted, showing a pattern that cannot be explained by the absence or scarcity of raw material. This technical pattern can be traced across several Mousterian assemblages in the South-eastern Pyrenees, suggesting technocognitive continuity in the Middle Palaeolithic during the Upper Pleistocene, in which changes in lithic reduction patterns are not evident. In this paper, the implications of such observations are contextualized within the general discussion on the behaviour of the South-eastern Pyrenees Neanderthals. 相似文献
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A detailed analysis of Bupleurum fruticosum oil was carried out by combination of GC (RI), GC/MS, and 13C‐NMR analyses. After fractionation by column chromatography, 34 components accounting for 97.8% of the oils were identified. The main component was β‐phellandrene (67.7%), followed by sabinene (9.3%), and limonene (5.6%). The evolution of the chemical composition according to the stages of development of the plant was investigated as well as the composition of leaf, twig, and flower oils. A solvent‐free microwave extraction (SFME) of aerial parts was carried out and the composition of the extract compared with that of the essential oil. Finally, 57 oil samples isolated from aerial parts of individual plants, collected all around Corsica, were analyzed, and the data were submitted to statistical analysis. Although the contents of the main components varied, only one group emerged, accompanied with some atypical compositions. 相似文献
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Andreas Birbach Emilio Casanova Johannes A. Schmid 《Genesis (New York, N.Y. : 2000)》2009,47(11):757-764
Tissue‐specific transgene expression in the prostate epithelium has previously been achieved using short prostate‐specific promoters, rendering transgenic mouse lines susceptible to integration site‐dependent effects. Here we demonstrate the applicability of bacterial artificial chromosome (BAC) technology to transgene expression in the prostate epithelium. We present mouse lines expressing an inducible Cre protein (MerCreMer) under the control of regulatory elements of the probasin gene on a BAC. These mouse lines show high organ specificity, high transgene expression in anterior, dorsal and lateral prostate lobes, no background Cre recombination using a reporter strain and adjustable amounts of Cre‐induced recombination upon tamoxifen induction. Together with two recently reported transgenic lines expressing the Cre‐ERT2 protein from small prostate‐specific promoters, these mouse lines will be useful in research focused on prostate‐specific disorders such as benign hyperplasia or cancer. genesis 47:757–764, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
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Verónica Veses Manuel Casanova Amelia Murgui Neil A.R. Gow & José P. Martínez 《FEMS yeast research》2009,9(2):293-300
The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis. 相似文献
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Marta Rodríguez-García Núria Climent Harold Oliva Víctor Casanova Rafael Franco Agathe Leon José M. Gatell Felipe García Teresa Gallart 《PloS one》2010,5(2)