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101.
Calleida desenderi Casale, sp. n., is described from Ecuador, Napo Province, surroundings of San Rafael. The new taxon is mostly characterized by the head and appendages rufous, the disc of elytra with marked metallic green reflection, the median lobe of aedeagus ring-like, and the endophallus with a long, twisted flagellum. A key for identification of the closer Neotropical species described so far is also provided. 相似文献
102.
Typhloreicheia monachasp. n. and Typhloreicheia ilianaesp. n. are described from two caves of Central-Eastern Sardinia (Nuoro province): the Bue Marino cave and the Nurra 'e Pradu cave, respectively. Both caves are located in the part of the island where many highly specialised subterranean carabid beetles are localised. Typhloreicheia monacha is apparently related to two other species of the same area, i.e. Typhloreicheia onnisi Casale & Magrini, 2004 and Typhloreicheia elegans (Dodero, 1916); Typhloreicheia ilianae is closely related to Typhloreicheia henroti Jeannel, 1957, known from a cave near Dorgali. Relationships and diagnostic features among these taxa are discussed and illustrated, and a key for identification of the specialised subterranean Typhloreicheia species of Sardinia is provided. The hypothesis of adaptive radiation of Reicheiina species in Sardinia, recently proposed by the senior author of this contribution, is further elaborated in light of new data. 相似文献
103.
Immacolata Andolfo Vito A. Lasorsa Francesco Manna Barbara E. Rosato Daniela Formicola Achille Iolascon Mario Capasso 《Journal of cellular and molecular medicine》2020,24(11):6459-6471
Neuroblastoma (NB) is the most common extracranial neoplasm in children. The overall outcome for high-risk NB patients is still unacceptable, therefore, it is critical to deeply understand molecular mechanisms associated with NB, which in turn can be utilized for developing drugs towards the treatment of NB. Protein kinases (TKs) play an essential role in the regulation of cell survival and proliferation. Different kinases, such as anaplastic lymphoma kinase (ALK), Aurora kinase, RET receptor tyrosine kinase, are potential therapeutic targets in various cancers, including NB. We analysed a cohort of 45 high-risk NB patients and 9 NB cell lines by a targeted—(t)NGS custom gene panel (genes codifying for the kinase domains of 90 TKs). We identified somatic variants in four TK genes (ALK, EPHB4, LMTK3 and EPHB6) in NB patients and we functionally characterized an interesting somatic variant, V871I, in EPHB4 gene. EPHB4 plays a crucial role in cardiovascular development and regulates vascularization in cancer-promoting angiogenesis, tumour growth and metastasis. Several EPHB4 mutations have previously been identified in solid and haematological tumour specimens but EPHB4 mutations were not described until now in NB. Interestingly, a re-analysis of public CGH-array showed that the EPHB4 gain is associated with advanced diseases in NB. We further demonstrated that higher EPHB4 expression is correlated to stage 4 of NB and with poor overall survival. Additionally, we also revealed that the EPHB4-V871I accounts for increased proliferation, migration and invasion properties in two NB cell lines by acting on VEGF, c-RAF and CDK4 target genes and by increasing the phosphorylation of ERK1-2 pathway. The use of two EPHB4 inhibitors, JI-101 and NVP-BHG712, was able to rescue the phenotype driven by the variant. Our study suggested that EPHB4 is a promising therapeutic target in high-risk NB. 相似文献
104.
Chiara Nicolò Gabriele Di Sante Annabella Procoli Giuseppe Migliara Alessia Piermattei Mariagrazia Valentini Giovanni Delogu Achille Cittadini Gabriela Constantin Francesco Ria 《PloS one》2013,8(2)
DC deliver information regulating trafficking of effector T cells along T-cell priming. However, the role of pathogen-derived motives in the regulation of movement of T cells has not been studied. We hereinafter report that amount of M tuberculosis in the adjuvant modulates relocation of PLP139-151 specific T cells. In the presence of a low dose of M tuberculosis in the adjuvant, T cells (detected by CDR3 BV-BJ spectratyping, the so-called “immunoscope”) mostly reach the spleen by day 28 after immunization (“late relocation”) in the SJL strain, whereas T cells reach the spleen by d 14 with a high dose of M tuberculosis (“early relocation”). The C57Bl/6 background confers a dominant “early relocation” phenotype to F1 (SJL×C57Bl/6) mice, allowing early relocation of T cells in the presence of low dose M tuberculosis. A single non-synonymous polymorphism of TLR2 is responsible for “early/late” relocation phenotype. Egress of T lymphocytes is regulated by TLR2 expressed on T cells. Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand. 相似文献
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Francesco Casuscelli Elena Ardini Nilla Avanzi Elena Casale Giovanni Cervi Matteo D’Anello Daniele Donati Daniela Faiardi Ronald D. Ferguson Gianpaolo Fogliatto Arturo Galvani Aurelio Marsiglio Danilo G. Mirizzi Marisa Montemartini Christian Orrenius Gianluca Papeo Claudia Piutti Barbara Salom Eduard R. Felder 《Bioorganic & medicinal chemistry》2013,21(23):7364-7380
A novel series of PIM inhibitors was derived from a combined effort in natural product-inspired library generation and screening. The novel pyrrolo[1,2-a]pyrazinones initial hits are inhibitors of PIM isoforms with IC50 values in the low micromolar range. The application of a rational optimization strategy, guided by the determination of the crystal structure of the complex in the kinase domain of PIM1 with compound 1, led to the discovery of compound 15a, which is a potent PIM kinases inhibitor exhibiting excellent selectivity against a large panel of kinases, representative of each family. The synthesis, structure–activity relationship studies, and pharmacokinetic data of compounds from this inhibitor class are presented herein. Furthermore, the cellular activities including inhibition of cell growth and modulation of downstream targets are also described. 相似文献
107.
Patrick Faubert Päivi Tiiva Tchamga Achille Nakam Jarmo K. Holopainen Toini Holopainen Riikka Rinnan 《Biogeochemistry》2011,106(3):503-516
Boreal peatlands have significant emissions of non-methane biogenic volatile organic compounds (BVOCs). Climate warming is
expected to affect these ecosystems both directly, with increasing temperature, and indirectly, through water table drawdown
following increased evapotranspiration. We assessed the combined effect of warming and water table drawdown on the BVOC emissions
from boreal peatland microcosms. We also assessed the treatment effects on the BVOC emissions from the peat soil after the
7-week long experiment. Emissions of isoprene, monoterpenes, sesquiterpenes, other reactive VOCs and other VOCs were sampled
using a conventional chamber technique, collected on adsorbent and analyzed by GC–MS. Carbon emitted as BVOCs was less than
1% of the CO2 uptake and up to 3% of CH4 emission. Water table drawdown surpassed the direct warming effect and significantly decreased the emissions of all BVOC
groups. Only isoprene emission was significantly increased by warming, parallel to the increased leaf number of the dominant
sedge Eriophorum vaginatum. BVOC emissions from peat soil were higher under the control and warming treatments than water table drawdown, suggesting
an increased activity of anaerobic microbial community. Our results suggest that boreal peatlands could have concomitant negative
and positive radiative forcing effects on climate warming following the effect of water table drawdown. The observed decrease
in CH4 emission causes a negative radiative forcing while the increase in CO2 emission and decrease in reactive BVOC emissions, which could reduce the cooling effect induced by the lower formation rate
of secondary organic aerosols, both contribute to increased radiative forcing. 相似文献
108.
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Graeme C. Hays Helen Bailey Steven J. Bograd W. Don Bowen Claudio Campagna Ruth H. Carmichael Paolo Casale Andre Chiaradia Daniel P. Costa Eduardo Cuevas P.J. Nico de Bruyn Maria P. Dias Carlos M. Duarte Daniel C. Dunn Peter H. Dutton Nicole Esteban Ari Friedlaender Kimberly T. Goetz Ana M.M. Sequeira 《Trends in ecology & evolution》2019,34(5):459-473