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491.
Colonization of nascent, deep-sea hydrothermal vents by a novel Archaeal and Nanoarchaeal assemblage
McCliment EA Voglesonger KM O'Day PA Dunn EE Holloway JR Cary SC 《Environmental microbiology》2006,8(1):114-125
Active deep-sea hydrothermal vents are areas of intense mixing and severe thermal and chemical gradients, fostering a biotope rich in novel hyperthermophilic microorganisms and metabolic pathways. The goal of this study was to identify the earliest archaeal colonizers of nascent hydrothermal chimneys, organisms that may be previously uncharacterized as they are quickly replaced by a more stable climax community. During expeditions in 2001 and 2002 to the hydrothermal vents of the East Pacific Rise (EPR) (9 degrees 50'N, 104 degrees 17'W), we removed actively venting chimneys and in their place deployed mineral chambers and sampling units that promoted the growth of new, natural hydrothermal chimneys and allowed their collection within hours of formation. These samples were compared with those collected from established hydrothermal chimneys from EPR and Guaymas Basin vent sites. Using molecular and phylogenetic analysis of the 16S rDNA, we show here that at high temperatures, early colonization of a natural chimney is dominated by members of the archaeal genus Ignicoccus and its symbiont, Nanoarchaeum. We have identified 19 unique sequences closely related to the nanoarchaeal group, and five archaeal sequences that group closely with Ignicoccus. These organisms were found to colonize a natural, high temperature protochimney and vent-like mineral assemblages deployed over high temperature outflows within 92 h. When compared phylogenetically, several of these colonizing organisms form a unique clade independent of those found in mature chimneys and low-temperature mineral chamber samples. As a model ecosystem, the identification of pioneering consortia in deep-sea hydrothermal vents may help advance the understanding of how early microbial life forms gained a foothold in hydrothermal systems on early Earth and potentially on other planetary bodies. 相似文献
492.
Cary A. Kuliasha John A. Finlay Sofia C. Franco Anthony S. Clare Shane J. Stafslien 《Biofouling》2017,33(3):252-267
There is currently strong motivation due to ecological concerns to develop effective anti-biofouling coatings that are environmentally benign, durable, and stable for use by the maritime industry. The antifouling (AF) and fouling-release (FR) efficacy of amphiphilic, charged copolymers composed of ~52% acrylamide, ~34% acrylic acid, and ~14% methyl acrylate grafted to poly(dimethyl siloxane) (PDMSe) surfaces were tested against zoospores of the green alga Ulva linza and the diatom Navicula incerta. The biofouling response to molecular weight variation was analyzed for grafts ranging from ~100 to 1,400 kg mol?1, The amphiphilic coatings showed a marked improvement in the FR response, with a 55% increase in the percentage removal of diatoms and increased AF efficacy, with 92% reduction in initial attachment density of zoospores, compared to PDMSe controls. However, graft molecular weight, in the range tested, was statistically insignificant. Grafting copolymers to PDMSe embossed with the Sharklet? microtopography did not produce enhanced AF efficacy. 相似文献
493.
494.
Assimilation of carbon and nitrogen from pollen and nectar by a predaceous larva and its effects on growth and development 总被引:7,自引:0,他引:7
Joseph M. Patt Sam C. Wainright George C. Hamilton Dexter Whittinghill Keith Bosley Jan Dietrick James H. Lashomb 《Ecological Entomology》2003,28(6):717-728
Abstract. 1. Predaceous insects may benefit from feeding on non‐prey foods, such as pollen, nectar, and honeydew, because they can provide nutrients that help maintain metabolism and enhance overall nutrient intake. Yet, the extent to which predaceous insects can assimilate non‐prey food and the importance of diet mixing during particular life history stages is poorly understood. In this study the relative contribution of an omnivorous diet to the growth and survivorship of a predaceous larva was tested in a hypothetical situation in which nutritionally optimal prey was not available. The study system comprised a predaceous larva (second‐ and third‐instar larvae of the green lacewing Chrysoperla carnea), nutritionally poor prey (larvae of Drosophila melanogaster), and non‐prey food (pollen suspension, a mixture of bee pollen and artificial nectar (1 M sucrose solution)). Chrysoperla carnea larvae in the mixed diet treatment were provided with both Drosophila larvae and pollen suspension, while those reared on the prey and non‐prey diet treatments received only Drosophila larvae or pollen suspension respectively. 2. The inclusion of pollen and sucrose in their diet enhanced the growth of C. carnea larvae. Second instars reared on the mixed diet developed significantly faster than their cohorts reared on the prey diet, however third instars reared on the mixed diet did not develop faster than their cohorts reared on the prey diet. Larvae reared on the mixed diet became larger adults than did those reared on either the prey or non‐prey diets. Third instars reared on the non‐prey diet completed their development while second instars in the non‐prey diet treatment failed to pupate. 3. Stable isotope analysis indicated that the larvae obtained most of their carbon (55–73%) and nitrogen (71–73%) from Drosophila but acquired only a minor amount of carbon (2–5%) and nitrogen (3–11%) from pollen. Larvae reared on the mixed and non‐prey diets acquired a relatively significant amount of carbon (23–51%) from sucrose. 4. A model, which included a novel fractionation factor to account for the isotopic effect of metamorphosis, was developed to explain the proportion of larval growth attributable to each diet item. It explained the adult δ13C values to within 0.2‰ and adult δ15N values to within 0.7‰ in all treatments. 5. Adults fed 15N‐labelled pollen as larvae retained the 15N signal of the pollen as adults. 6. The collective results of this study support the view that, despite their dependence on prey arthropods to obtain most of their dietary nitrogen, omnivorous lacewing larvae can enhance their growth and development by supplementing their diets with alternative non‐prey food resources. This finding is consistent with the notion that omnivory has evolved as a feeding strategy to acquire both additional nitrogen as well as trace nutrients. 相似文献
495.
Molecular genetic evidence for the involvement of a specific polygalacturonase, P2c, in the invasion and spread of Aspergillus flavus in cotton bolls. 总被引:3,自引:0,他引:3 下载免费PDF全文
M T Shieh R L Brown M P Whitehead J W Cary P J Cotty T E Cleveland R A Dean 《Applied microbiology》1997,63(9):3548-3552
Isolates of Aspergillus flavus can be differentiated based on production of the polygalacturonase P2c. One group of isolates produces P2c, whereas the other group does not. In general, the group that produces P2c causes more damage and spreads to a greater extent in cotton bolls than those isolates that do not produce P2c. To determine whether P2c contributes to disease, the expression of pecA, the gene previously determined to encode P2c, was genetically altered. Adding the pecA gene to a strain previously lacking the gene resulted in the ability to cause significantly more damage to the intercarpellary membrane and the ability spread to a greater extent within the adjacent locule compared to the abilities of a control transformant. Conversely, eliminating the expression of pecA by targeted disruption caused a significant reduction in aggressiveness compared to that of a nondisrupted control transformant. These results provide direct evidence that P2c contributes to the invasion and spread of A. flavus during infection of cotton bolls. However, other factors not evaluated in this study also contribute to aggressiveness. 相似文献
496.
497.
Genetic variation among endosymbionts of widely distributed vestimentiferan tubeworms 总被引:2,自引:0,他引:2
Di Meo CA Wilbur AE Holben WE Feldman RA Vrijenhoek RC Cary SC 《Applied and environmental microbiology》2000,66(2):651-658
Vestimentiferan tubeworms thriving in sulfidic deep-sea hydrothermal vents and cold seeps are constrained by their nutritional reliance on chemoautotrophic endosymbionts. In a recent phylogenetic study using 16S ribosomal DNA, we found that endosymbionts from vent and seep habitats form two distinct clades with little variation within each clade. In the present study, we used two different approaches to assess the genetic variation among biogeographically distinct vestimentiferan symbionts. DNA sequences were obtained for the noncoding, internal transcribed spacer (ITS) regions of the rRNA operons of symbionts associated with six different genera of vestimentiferan tubeworms. ITS sequences from endosymbionts of host genera collected from different habitats and widely distributed vent sites were surprisingly conserved. Because the ITS region was not sufficient for distinguishing endosymbionts from different habitats or locations, we used a DNA fingerprinting technique, repetitive-extragenic-palindrome PCR (REP-PCR), to reveal differences in the distribution of repetitive sequences in the genomes of the bacterial endosymbionts. Most of the endosymbionts displayed unique REP-PCR patterns. A cladogram generated from these fingerprints reflected relationships that may be influenced by a variety of factors, including host genera, geographic location, and bottom type. 相似文献
498.
499.
A 40–50% reduction in soybean [ Glycine max (L.) Merr. cv. Century 84] hypocotyl elongation occurred 24 h after application of mechanical stress. Exogenous at 10 m M inhibited growth by 28% if applied with the Ca2+ ionophore A23187 to the zone of maximum hypocotyl elongation. La3+ was even more inhibitory than Ca2+ , especially above 5 m M . Treatment with ethyleneglycol-bis-(β3-aminoethylether)-N, N, N', N'-tetraacetic acid (EGTA) alone had no effect on growth of non-stressed seedlings at the concentrations used but negated stress-induced growth reduction by 36% at 4 m M when compared to non-treated, stressed controls. Treatment with EDTA was ineffective in negating stress-induced growth inhibition. Calmodulin antagonists calmidazolium, chlorpromazine, and 48/80 also negated stress-induced growth reduction by 23, 50, and 35%, respectively. 相似文献
500.
Maternal smoking during pregnancy affects neuroendocrine cells in the neonate hamster lung 总被引:1,自引:0,他引:1
Primigravid Syrian golden hamsters were exposed in a Walton smoking machine to the smoke from either weak or strong cigarettes for 10 minute periods, 4 times a day from the 3rd to 14th (2nd last) day of pregnancy. Control hamsters were either similarly restrained in a Walton machine equipped with an unlit cigarette, or were not placed in the machine or restrained. Examination of the progeny in the first 6 days of life showed changes in density indices of grouped pulmonary neuroendocrine (NE) cells (neuroepithelial bodies, NEB) that were related to in utero exposure to maternal smoking. Argyrophil NEB were more numerous, larger, and contained more cells at birth among neonates whose mothers smoked the strong cigarette (2.45 mg nicotine and 36.8 mg tar) during pregnancy. This suggests a dose-related effect as the weak cigarette (0.37 mg nicotine and 33.8 mg tar) group did not show such changes. However, some of the changes described did not last through 3 or 6 days of age. The stress resulting from restraint alone also appeared to increase argyrophil NEB indices. Lung tissue volume fraction was increased in the weak cigarette group over all other groups at birth and 3 days; this suggests that low nicotine has the strongest pharmacological effect on lung tissue growth. The medial thickness of pulmonary arterioles was unchanged by either treatment; this provides morphometric evidence that chronic pulmonary hypertension was not present. We could not determine whether the increased NEB indices were caused by increased stainability, by activation of resident reserve cells, or by actual mitosis. 相似文献