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91.
Caruso M. Fiore C. Contursi M. Salzano G. Paparella A. Romano P. 《World journal of microbiology & biotechnology》2002,18(2):159-163
This work deals with biogenic amine production by yeast strains isolated from grapes and wines. A total of 50 strains were tested for their capacity to produce biogenic amines in wine. In general, all the species produced very low or non-detectable amounts of histamine, whereas methylamine and agmatine were formed by all the species considered. The highest concentration of total biogenic amines was formed by Brettanomyces bruxellensis, with an average value of 15 mg/l, followed by Saccharomyces cerevisiae with an average of 12.14 mg/l. The other species formed less than 10 mg of total biogenic amines per litre. Wines fermented with the most fermentative strains of S. cerevisiae species had the highest contents of ethanolamine, from 2.3 to 16 mg/l, and of agmatine, from 3.1 to 7.5 mg/l. The strains of the other species, which exhibited a low fermentative ability, Kloeckera apiculata, B. bruxellensis and Metschnikowia pulcherrima, varied in the production of agmatine and phenylethylamine. A significant variability in the production of cadaverine was characteristic of Candida stellata strains, which varied also in ethanolamine production. Our results emphasize the importance of using selected strains of S. cerevisiae, not only for the expression of desirable technological traits, but also to avoid potentially negative effects on human health. Therefore, the characterization of strains of S. cerevisiae for the 'production of biogenic amines' becomes of applicative interest. 相似文献
92.
Federico?LicastroEmail author Giuseppina?Candore Domenico?Lio Elisa?Porcellini Giuseppina?Colonna-Romano Claudio?Franceschi Calogero?Caruso 《Immunity & ageing : I & A》2005,2(1):8
The process of maintaining life for the individual is a constant struggle to preserve his/her integrity. This can come at
a price when immunity is involved, namely systemic inflammation. Inflammation is not per se a negative phenomenon: it is the
response of the immune system to the invasion of viruses or bacteria and other pathogens. During evolution the human organism
was set to live 40 or 50 years; today, however, the immune system must remain active for much a longer time. This very long
activity leads to a chronic inflammation that slowly but inexorably damages one or several organs: this is a typical phenomenon
linked to ageing and it is considered the major risk factor for age-related chronic diseases. Alzheimer's disease, atherosclerosis,
diabetes and even sarcopenia and cancer, just to mention a few – have an important inflammatory component, though disease
progression seems also dependent on the genetic background of individuals. Emerging evidence suggests that pro-inflammatory
genotypes are related to unsuccessful ageing, and, reciprocally, controlling inflammatory status may allow a better chance
of successful ageing. In other words, age-related diseases are "the price we pay" for a life-long active immune system: this
system has also the potential to harm us later, as its fine tuning becomes compromised. Our immune system has evolved to control
pathogens, so pro-inflammatory responses are likely to be evolutionarily programmed to resist fatal infections with pathogens
aggressively. Thus, inflammatory genotypes are an important and necessary part of the normal host responses to pathogens in
early life, but the overproduction of inflammatory molecules might also cause immune-related inflammatory diseases and eventually
death later. Therefore, low responder genotypes involved in regulation of innate defence mechanisms, might better control
inflammatory responses and age-related disease development, resulting in an increased chance of long life survival in a "permissive"
environment with reduced pathogen load, medical care and increased quality of life. 相似文献
93.
Amari G Armani E Ghirardi S Delcanale M Civelli M Caruso PL Galbiati E Lipreri M Rivara S Lodola A Mor M 《Bioorganic & medicinal chemistry》2004,12(14):3763-3782
The synthesis, binding affinity for estrogen receptor subtypes (ER alpha and ER beta) and pharmacological activity on rat uterus of a new class of potent ligands, characterized by a 3-phenylbenzopyran scaffold with a basic side chain in position 4, are reported. Some of these compounds, endowed with very high receptor affinity, showed potent inhibition of agonist-stimulated uterine growth, with no or limited proliferative effect. Binding affinity mostly depended on the nature and position of substituents at the 3-phenyl ring, while the uterine activity seems to be affected by basic chain length. Compound 9c (CHF4227) showed excellent binding affinity and antagonist activity on the uterus. The docking of benzopyran derivatives explained the structure-affinity relationships observed for 3-phenyl substitution: a small, hydrophobic 4'-substituent could interact with a small accessory binding cavity, while di-substitution at 4' and 3' led to some ER alpha selectivity. This selectivity can be ascribed to differences in amino acid composition and side chain conformation in the region accommodating the 3-phenyl ring at human ER alpha and ER beta ligand-binding domain. 相似文献
94.
Zhang Q Zulfiqar F Xiao X Riazuddin SA Ahmad Z Caruso R MacDonald I Sieving P Riazuddin S Hejtmancik JF 《Human genetics》2007,122(3-4):293-299
Mutation in the PROM1 gene previously has been identified in one family with retinal degeneration for which neither ERG recordings
nor detailed information about visual impairment is available. A large family with multiple individuals affected by retinal
degeneration was ascertained in the Punjab province of Pakistan. The visual acuity of all affected patients in the family
was severely compromised beginning in early childhood. The retinal disease in this family is a severe form of retinitis pigmentosa
(RP) accompanied by macular degeneration. Fundus changes advanced with age. Choriocapillaris atrophy and posterior RPE atrophy
were obvious allowing visualization of the large choroidal vessels in patients over 40 years of age. Rod and cone responses
on ERG recordings were extinguished in patient’s teens. A genome-wide scan mapped the disease to a 34.7 cM region of chromosome
4p14–p16 between D4S1599 and D4S405. A maximum lod score of 3.96 with D4S403 and D4S391 is seen at θ = 0. Sequence analysis
of PROM1 located in the linkage interval identified a c.1726C>T homozygous transition in exon 15: resulting in p.Gln576X in
the translated protein. This mutation is found in a homozygous state in all six affected individuals and was heterozygous
in five of the six unaffected family members examined. The mutation was not detected in 192 chromosomes of unrelated control
individuals of the same ethnicity and from the same region. This delineates the phenotypic characteristics of retinopathy
caused by mutations in PROM1.
Qingjiong Zhang, Fareeha Zulfiqar, Xueshan Xiao, Sheikh Riazuddin and J. Fielding Hejtmancik contributed equally. 相似文献
95.
Annibale A. Puca Anna Ferrario Anna Maciag Giulia Accardi Anna Aiello Caterina Maria Gambino Giuseppina Candore Calogero Caruso Aryan M. Namboodiri Janardan P. Pandey 《Immunity & ageing : I & A》2018,15(1):26
Background
The aim of this study was to analyse the role of GM allotypes, i.e. the hereditary antigenic determinants expressed on immunoglobulin polypeptide chains, in the attainment of longevity. The role played by immunoglobulin allotypes in the control of immune responses is well known as well as the role of an efficient immune response in longevity achievement. So, it is conceivable that particular GM allotypes may contribute to the generation of an efficient immune response that supports successful ageing, hence longevity.Methods
In order to show if GM allotypes play a role in the achievement of longevity, we typed the DNA of 95 Long-living individuals (LLIs) and 96 young control individuals (YCs) from South Italy for GM3/17 and GM23+/? alleles.Results
To demonstrate the role of GM allotypes in the attainment of longevity we compared genotype and allele frequencies of GM allotypes between LLIs and YCs. A global chi-square test (3?×?2) shows that the distribution of genotypes at the GM 3/17 locus is highly significantly different in LLIs from that observed in YCs (p?<?0.0001). The 2?×?2 chi-square test shows that the carriers of the GM3 allele contribute to this highly significant difference. Accordingly, GM3 allele is significantly overrepresented in LLIs. No significant differences were instead observed regarding GM23 allele.Conclusion
These preliminary results show that GM3 allotype is significantly overrepresented in LLIs. To best of our knowledge, this is the first study performed to assess the role of GM allotypes in longevity. So, it should be necessary to verify the data in a larger sample of individuals to confirm GM role in the attainment of longevity.96.
97.
98.
Caruso JF Hernandez DA Porter A Schweikert T Saito K Cho M De Garmo N Nelson NM 《Journal of strength and conditioning research / National Strength & Conditioning Association》2006,20(1):151-156
Integrated electromyography (IEMG) and performance outcomes from resistance exercise may be influenced by gender, repetition order, and contractile mode. A novel strength training apparatus employing inertial resistance operates unlike standard exercise equipment and may therefore evoke different IEMG and performance outcomes. Subjects performed 3-set, 10-repetition calf press workouts on an inertial device while IEMG and flywheel velocity were recorded. Dependent variables were examined with 2 (men, women) x 2 (first vs. 10th repetition) x 2 (concentric, eccentric) analyses of variance. Performance outcomes showed gender-by-repetition and gender-by-contractile-mode interactions, with men's 10th-repetition and men's concentric data, respectively, causing these effects. Medial gastrocnemius (MG) IEMG showed a gender-by-repetition interaction resulting from men's first-repetition data. Greater 10th-repetition performance despite higher first-repetition MG IEMG may result from heightened triceps surae elastic energy utilization and/or maintained cross-bridges during transitions from lengthening-to-shortening actions. Inertial strength training may improve performance outcomes without additional motor unit recruitment. 相似文献
99.
Portincasa P Grattagliano I Testini M Caruso ML Wang DQ Moschetta A Calamita G Vacca M Valentini AM Renna G Lissidini G Palasciano G 《Free radical biology & medicine》2007,42(9):1381-1391
Whereas long-term cholestasis results in intestinal alterations and increased permeability to hepatotoxins, the effect of short-term cholestasis is less known and was investigated in bile duct ligated (BDL) rats. In the intestinal mucosa, at Day 7 BDL, total glutathione and protein sulfhydryl contents had decreased, oxidized glutathione levels increased (P<0.05 vs baseline), and a reduced epithelium thickness with dissolving crypt phenomena was observed in 40% of rats. At Day 10, total protein content, glutathione-related enzyme activities, and the transmural electrophysiological activity had decreased (-50%); by contrast, oxidized proteins doubled (P<0.05), and histological changes were extended to 70% of rats. In vitro exposure to taurodeoxycholate at micellar concentrations determined dysepithelization in normal gut but dissolving crypt phenomena and necrosis in cholestatic bowels. In the liver, ongoing cholestasis was associated with early oxidative changes especially in mitochondria, where protein sulfhydryls were decreased and negatively correlated with glutathione-protein mixed disulfides (r=-0.807, P<0.001). Daily oral administration of tauroursodeoxycholate, a hydrophilic bile salt, and glutathione to BDL rats improved intestinal histology, function, and redox state. In conclusion, short-term cholestasis results in distinctive functional, oxidative, and morphological changes of intestinal mucosa, determined increased vulnerability to toxic injury, and parallel hepatic oxidative damage. 相似文献