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81.
Linda Randsalu-Wendrup Daniel J. Conley Jacob Carstensen Ian Snowball Catherine Jessen Sherilyn C. Fritz 《Ecosystems》2012,15(8):1336-1350
A detailed diatom record from Lake K?lksj?n, west-central Sweden, reveals two periods of abrupt ecological change correlative with the 8.2?ka cooling event. Using a combination of abrupt step changes and piece-wise linear regressions, the diatom data were analyzed for change points over time, and two sudden and large events that are described as regime shifts were detected. During the first event at c. 8040?cal. y BP, a doubling in diatom biomass took place over 5?C10?years. This increase in primary productivity can be connected to an erosion event in the catchment that resulted in an abrupt increase in nutrient supply to the lake. The second event was characterized by a substantial shift within the planktonic diatom community from taxa indicative of colder conditions to those indicating warm over 5?C10?years at c. 7850?cal. y BP. This event was superimposed on a successive change from periphytic to planktonic diatom dominance over a 250-year period and a gradual diversification of the periphytic community that spanned c. 150?years. Rapid climate warming following the 8.2?ka event likely caused these changes and both regime shifts are examples of externally driven abrupt ecological change. This study demonstrates that it is possible to detect, quantify and test for regime shifts in paleoecological data, and it highlights the need for high sampling resolution and precise chronological control. High-resolution paleoecological reconstructions of ecological regime shifts in response to climate change can provide useful analogues of future changes in ecosystem structure and functioning. 相似文献
82.
Susi M. C. Edrén Signe M. Andersen Jonas Teilmann Jacob Carstensen Pernille B. Harders Rune Dietz Lee A. Miller 《Marine Mammal Science》2010,26(3):614-634
This study investigates the effects of the construction and operation of a large Danish offshore wind farm on harbor and gray seal haul-out behavior within a nearby (4 km) seal sanctuary. Time-lapse photography, visual monitoring, and aerial surveys were used to monitor the number of seals on land in daylight hours. Seals were monitored during two preconstruction periods (19 June–31 August 2001 and April–August 2002), a construction period of the wind farm (August 2002–December 2003), and a period of operation of the wind farm (December 2003–December 2004). Monthly aerial surveys were conducted to estimate the proportion of seals in the sanctuary relative to neighboring haul-out sites. From preconstruction to construction and through the first year of operation the number of harbor seals in the sanctuary increased at the same rate as the number of seals at the neighboring haul-out sites. No long-term effects on haul-out behavior were found due to construction and operation of the wind farm. However, a significant short-term decrease was seen in the number of seals present on land during sheet pile driving in or near the wind farm. Acoustic deterrents were utilized simultaneously to avoid hearing damage. 相似文献
83.
84.
Passive Electrical Properties of Microorganisms: I. Conductivity of Escherichia coli and Micrococcus lysodeikticus
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Effective conductivities are reported for the bacteria Escherichia coli and Micrococcus lysodeikticus over a range of environmental conductivity. The apparent conductivities of the organisms can be explained in terms of the properties of the cell wall. At low conductivities of the environment, the conductivity of the cell appears to be dominated by the counterions of the fixed charge of the cell wall. At higher conductivities of the suspending medium, evidence suggests that ions from the environment invade the cell wall causing an increase in the effective conductivity of the cell so that it takes on values roughly proportional to that of the environment. The model points to the usefulness of dielectric techniques in studies of the properties of intact cell walls. 相似文献
85.
A. V. Prasad M. W. Miller E. L. Carstensen Ch. Cox M. Azadniv A. A. Brayman 《Radiation and environmental biophysics》1991,30(4):305-320
Summary An attempt was made to verify a report (Liboff et al. 1987) that a unique combination of DC and AC magnetic field exposures at room temperature results in a 3-fold increase in45Ca2+ uptake by human lymphocytes in vitro. Exposures at resonance condition, as well as at frequencies and amplitudes above and below the reported effective exposure conditions, were without effect, as were exposures at 37° C. Treatment with ionomycin (0.25 µM), a positive control, resulted in a highly significant increase45Ca2+ uptake. Some experiments were performed simultaneously by different investigators. Their results did not differ significantly. All experiments were conducted double blindly. 相似文献
86.
Structural insight into the function of myelin basic protein as a ligand for integrin alpha M beta 2
Stapulionis R Oliveira CL Gjelstrup MC Pedersen JS Hokland ME Hoffmann SV Poulsen K Jacobsen C Vorup-Jensen T 《Journal of immunology (Baltimore, Md. : 1950)》2008,180(6):3946-3956
Multiple sclerosis (MS) is an inflammatory disease where phagocytic cells infiltrate the nerve tissue and act as terminal agents in destruction of the myelin sheath. However, the mechanism that triggers the ability of these cells to recognize myelin remains obscure. We show that myelin basic protein (MBP), a major autoantigen in MS, is a potent and specific ligand for the integrin alpha(M)beta(2) (Mac-1, CD11b/CD18) expressed mainly on phagocytic cells. MBP undergoes a dramatic conformational change when liberated from the lipid-rich environment of the myelin sheath. The MS drug glatiramer acetate mimics the conformationally labile regions of MBP, interacts in the unfolded state strongly with alpha(M)beta(2), and inhibits the MBP binding to alpha(M)beta(2). Our study reveals a link between MBP, glatiramer acetate, and the alpha(M)beta(2) integrin, and suggests a new model for MS pathogenesis based on the recognition of unfolded MBP by the alpha(M)beta(2) integrin. 相似文献
87.
Carstensen B 《Biostatistics (Oxford, England)》2004,5(3):399-413
In studies designed to compare different methods of measurement where more than two methods are compared or replicate measurements by each method are available, standard statistical approaches such as computation of limits of agreement are not directly applicable. A model is presented for comparing several methods of measurement in the situation where replicate measurements by each method are available. Measurements are viewed as classified by method, subject and replicate. Models assuming exchangeable as well as non-exchangeable replicates are considered. A fitting algorithm is presented that allows the estimation of linear relationships between methods as well as relevant variance components. The algorithm only uses methods already implemented in most statistical software. 相似文献
88.
Warming of Subarctic waters accelerates development of a key marine zooplankton Calanus finmarchicus
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Agata Weydmann Waldemar Walczowski Jacob Carstensen Sławomir Kwaśniewski 《Global Change Biology》2018,24(1):172-183
Recent observations confirm the rising temperatures of Atlantic waters transported into the Arctic Ocean via the West Spitsbergen Current (WSC). We studied the overall abundance and population structure of the North Atlantic keystone zooplankton copepod Calanus finmarchicus, which is the main prey for pelagic fish and some seabirds, in relation to selected environmental variables in this area between 2001 and 2011, when warming in the Arctic and Subarctic was particularly pronounced. Sampling within a 3‐week time window each summer demonstrated that trends in the overall abundance of C. finmarchicus varied between years, with the highest values in “extreme” years, due to high numbers of nauplii and early copepodite stages in colder years (2001, 2004, 2010), and contrary to that, the fifth copepodite stage (C5) peaking in warm years (2006, 2007, 2009). The most influential environmental variable driving C. finmarchicus life cycle was temperature, which promoted an increased C5 abundance when the temperature was above 6°C, indicating earlier spawning and/or accelerated development, and possibly leading to their development to adults later in the summer and spawning for the second time, given adequate food supply. Based on the presented high interannual and spatial variability, we hypothesize that under a warmer climate, C. finmarchicus may annually produce two generations in the southern part of the WSC, what in turn could lead to food web reorganization of important top predators, such as little auks, and induce northward migrations of fish, especially the Norwegian herring. 相似文献
89.
In coastal areas seagrasses have considerable ecological importance and respond to eutrophication pressures. Seagrasses have, therefore, become an important parameter for assessing ecological status of marine water bodies. In this study we analyzed the sources of uncertainty associated with the monitoring of the maximum depth limit of eelgrass (Zostera marina). Based on a long-term marine monitoring of eelgrass depth limit in Danish coastal waters we estimated the uncertainty contribution of years, divers, transects, and replicates in water bodies and their sub-areas. For all these components the absolute uncertainty increased with the maximum depth limit. We used either a Spheric or a Gaussian function to describe the relationship between uncertainty and the maximum depth limit for each variable. This parameterization of the depth-specific uncertainty allowed estimation of the total variance associated with monitoring, which can be used to evaluate monitoring designs. The variance components were included in a time budget for optimizing eelgrass monitoring. With a maximum time limit of 100 or 200 h allocated for monitoring, the design that resulted in the lowest variance of the estimated maximum depth limit used 2 or 3 divers, respectively, and involved 2 or 3 years of monitoring and 4–8 transects. 相似文献
90.
Maren Carstensen Claudia Wiza Karin R?hrig Pia Fahlbusch Michael Roden Christian Herder D. Margriet Ouwens 《PloS one》2014,9(1)
Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) α-treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p<0.05), but did not affect MCP-1 and adiponectin release. In MCP-1-treated hSkMC, Sfrp5 did not affect cytokine secretion. In untreated adipocytes, Sfrp5 decreased the insulin-mediated phosphorylation of Akt-Ser473, Akt-Thr308, GSK3α-Ser21 and PRAS40-Thr246 by 34% (p<0.01), 31% (p<0.05), 37% (p<0.05) and 34% (p<0.01), respectively, and the stimulation of glucose uptake by 25% (p<0.05). Incubation with TNFα increased the phosphorylation of JNK and NFκB, and impaired insulin signaling. When Sfrp5 and TNFα were combined, there was no additional effect on insulin signaling and JNK phosphorylation, but phosphorylation of NFκB was reversed to basal levels. Sfrp5 had no effect on insulin signaling in untreated or in MCP-1 treated hSkMC. Thus, Sfrp5 lowered IL-6 release and NFκB phosphorylation in cytokine-treated human adipocytes, but not under normal conditions, and decreased insulin signaling in untreated human adipocytes. Sfrp5 did not act on hSkMC. Therefore, the cellular actions of Sfrp5 seem to depend on the type of tissue as well as its inflammatory and metabolic state. 相似文献