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991.
美国生物质资源研究规划与举措分析及启示 总被引:3,自引:0,他引:3
发展生物质资源研究对于国家的可持续发展具有重要的战略意义。近年来,美国相继制订若干研究计划或发展路线图,并采取有效举措,持续推动生物质资源的研究、开发和利用。通过分析美国在生物质资源研究领域的重要举措,特别是分析其相关重要规划的具体内容,总结生物质资源研究面临的重要问题和研究热点,对发展我国生物质资源研究提出一些思考建议。 相似文献
992.
993.
Identification and characterization of NARROW AND
ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice 总被引:7,自引:0,他引:7
Jiang Hu Li Zhu Dali Zeng Zhenyu Gao Longbiao Guo Yunxia Fang Guangheng Zhang Guojun Dong Meixian Yan Jian Liu Qian Qian 《Plant molecular biology》2010,73(3):283-292
Leaf morphology is an important agronomic trait in rice breeding. We isolated three allelic mutants of NARROW AND ROLLED LEAF 1 (nrl1) which showed phenotypes of reduced leaf width and semi-rolled leaves and different degrees of dwarfism. Microscopic analysis indicated that the nrl1-1 mutant had fewer longitudinal veins and smaller adaxial bulliform cells compared with the wild-type. The NRL1 gene was mapped to the chromosome 12 and encodes the cellulose synthase-like protein D4 (OsCslD4). Sequence analyses revealed single base substitutions in the three allelic mutants. Genetic complementation and over-expression of the OsCslD4 gene confirmed the identity of NRL1. The gene was expressed in all tested organs of rice at the heading stage and expression level was higher in vigorously growing organs, such as roots, sheaths and panicles than in elsewhere. In the mutant leaves, however, the expression level was lower than that in the wild-type. We conclude that OsCslD4 encoded by NRL1 plays a critical role in leaf morphogenesis and vegetative development in rice. 相似文献
994.
Stearoyl-CoA Desaturase (SCD) Gene Polymorphism in Goat Breeds 总被引:1,自引:0,他引:1
Chun-Lei Zhang Xue-Yuan Gao Ru-Ying Shao Yan-Hong Wang Xing-Tang Fang Hong Chen 《Biochemical genetics》2010,48(9-10):822-828
995.
Linsheng Liu Jiye Aa Guangji Wang Bei Yan Xinwen Wang Bei Cao Mengjie Li Yuanting Zheng Fang Zhou Zimei Wu 《Analytical biochemistry》2010,406(2):105-6697
In metabolomic research, blood plasma and serum have been considered to possess similar compositions and properties. Their perceived equivalence has resulted in researchers choosing arbitrarily between serum and plasma for analysis. Here, routine serum and plasma were prepared and their low-molecular-weight compounds were determined using gas chromatography/time-of-flight mass spectrometry. Principal components analysis was applied to process the acquired data, and marked differences in metabolite profiles were observed between serum and plasma. Of the 72 identified compounds, 36 (50%) discriminate serum from plasma, with 29 and 7 metabolites showing a significantly higher abundance (t test, P < 0.05) in serum and plasma, respectively. Incubation of blood had distinct effects on the analyte peak areas, with the effects being more pronounced for plasma than for serum and more pronounced for a shorter incubation than for a longer incubation. These results highlight the importance in choosing serum or plasma as the analytical sample and in stipulating the incubation time. Because incubation affected the analyte peak areas less in serum than in plasma, we recommend serum as the sample of choice in metabolomic studies. 相似文献
996.
Chia-Cheng Hung Shee-Uan Chen Shin-Yu Lin Mei-Ya Fang Yi-Yi Tsai Yu-Shih Yang Yi-Ning Su 《Analytical biochemistry》2010,400(1):69-77
Preimplantation genetic diagnosis (PGD) is employed increasingly to allow transfer of embryos to the uterus in assisted reproduction procedures. There are three stages of biopsy: polar bodies, one or two blastomeres from the cleavage-stage embryos, and trophectoderm cells (∼5 cells) from the blastocyst-stage embryos. Validation of polymerase chain reaction (PCR)-based assays are challenging because only limited genetic material can be obtained for PGD. In the current study, we modified a valid single-cell PCR protocol for PGD using real-time PCR assay with fluorescence resonance energy transfer (FRET) hybridization probes followed by melting curve analysis. We optimized and clinically applied the protocol, permitting molecular genetic analysis to amplify a specific region on the beta-globin (HBB) gene for a couple, carriers of two mutations: c.-78A>G and c.52A>T. Among a total of eight embryos obtained after ovarian stimulation, a single blastomere per embryo at the six- to eight-cell stage was biopsied. This PGD method showed that four embryos were unaffected, two embryos were selected for transfer, and one pregnancy was achieved. Finally, a healthy male baby was delivered at 38 weeks’ gestation. The results obtained using the new method, FRET hybridization probes, were compared with findings using an existing method, primer extension minisequencing. 相似文献
997.
Y. L. Jiao L. H. Wang B. H. Jiao S. J. Wang Y. W. Fang S. Liu 《Applied Biochemistry and Microbiology》2010,46(2):148-153
The function of a new starter unit acyltransferase (SAT) domain SAT-EF080951 (GenBank accession number) encoded in a new type I polyketide synthase (PKS) gene cluster EF568935 (GenBank accession number) isolated for this study was analyzed by domain replacement with an extender unit AT (EAT) domain of avermectin PKS. It was shown that the SAT-EF080951 incorporated malonyl-CoA specifically in vivo, which contradicted the specificity that we had previously determined by substrate binding test in vitro. The result of this study indicates that type I PKS-SAT can alter its specificity in vivo and functions well in extender units and proved the feasibility of the SAT-EAT domain replacement in type I PKS. We propose that SAT-EAT replacement strategy could be a novel route for increasing the diversity of new polyketides combinatorially biosynthesized. The new type I PKS-SAT-EF080951 studied herein may be further employed for related studies on enzymology or combinatorial biosynthesis of polyketides. 相似文献
998.
目的:研究HLA-DRB1基因多态性与新疆哈萨克族人群结核病(TB)的相关性。方法:采用病例-对照的研究方法,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术对231例新疆哈萨克族肺结核患者和230例新疆哈萨克族健康对照者的13个HLA-DRB1等位基因进行分型,比较其等位基因频率(GF)并计算其比值比(OR)。结果:与新疆哈萨克族人群对照组相比,新疆哈萨克族人群结核病例组中HLA-DRB1*04显著增高(11.72%比6.75%,p0.05,OR=1.889),HLA-DRB1*10也增高(2.86%比1.09%),但统计学上无显著性差异(Pc0.05)。结论:HLA-DRB1*04可能是新疆哈萨克族人群结核病的易感基因。 相似文献
999.
The diploid species Brassica rapa(genome AA)and B.oleracea(genome CC)were compared by fuU-seale proteome analyses of seedling.A total of 28.2% of the proteins was common to both species,indicating the existence of a basal or ubiquitous proteome.How-ever,a number of discriminating proteins(32.0%)and specific proteins(39.8%)of the Brassica A and C genomes,respectively,were identified,which could represent potentially species-specific functions.Based on these A or C genome-specific proteins,a number of PCR-based markers to distinguish B.rapa and B.oleracea species were also developed. 相似文献
1000.
Huang Huang Na Wei Yingfei Xiong Feng Yang Huaqiang Fang Wenjun Xie Zhuan Zhou Heping Cheng Zicai Liang Quan Du 《生物学前沿》2010,5(3):272-281
To modulate gene expression in research studies or in potential clinical therapies, transfection of exogenous nucleic acids
including plasmid DNA and small interference RNA (siRNA) are generally performed. However, the cellular processing and the
fate of these nucleic acids remain elusive. By investigating the cellular behavior of transfected nucleic acids using confocal
imaging, here we show that when siRNA was co-transfected into cultured cells with other nucleic acids, including single-stranded
RNA oligonucleotides, single and double-stranded DNA oligonucleotides, as well as long double-stranded plasmid DNA, they all
aggregate in the same cytoplasmic granules. Interestingly, the amount of siRNA aggregating in granules was found not to correlate
with the gene silencing activity, suggesting that assembly of cytoplasmic granules triggered by siRNA transfection may be
separable from the siRNA silencing event. Our results argue against the claim that the siRNA-aggregating granules are the
functional site of RNA interference (RNAi). Taken together, our studies suggest that, independent of their types or forms,
extraneously transfected nucleic acids are processed through a common cytoplasmic pathway and trigger the formation of a new
type of cytoplasmic granules “transfection granules”. 相似文献