全文获取类型
收费全文 | 19340篇 |
免费 | 1773篇 |
国内免费 | 9篇 |
出版年
2023年 | 80篇 |
2022年 | 213篇 |
2021年 | 357篇 |
2020年 | 217篇 |
2019年 | 260篇 |
2018年 | 318篇 |
2017年 | 267篇 |
2016年 | 473篇 |
2015年 | 873篇 |
2014年 | 924篇 |
2013年 | 1137篇 |
2012年 | 1411篇 |
2011年 | 1370篇 |
2010年 | 923篇 |
2009年 | 812篇 |
2008年 | 1114篇 |
2007年 | 1120篇 |
2006年 | 1046篇 |
2005年 | 967篇 |
2004年 | 910篇 |
2003年 | 919篇 |
2002年 | 943篇 |
2001年 | 184篇 |
2000年 | 140篇 |
1999年 | 219篇 |
1998年 | 235篇 |
1997年 | 190篇 |
1996年 | 159篇 |
1995年 | 159篇 |
1994年 | 155篇 |
1993年 | 152篇 |
1992年 | 129篇 |
1991年 | 118篇 |
1990年 | 124篇 |
1989年 | 119篇 |
1988年 | 117篇 |
1987年 | 113篇 |
1986年 | 101篇 |
1985年 | 100篇 |
1984年 | 122篇 |
1983年 | 126篇 |
1982年 | 146篇 |
1981年 | 123篇 |
1980年 | 98篇 |
1979年 | 72篇 |
1978年 | 92篇 |
1977年 | 79篇 |
1976年 | 69篇 |
1975年 | 62篇 |
1974年 | 77篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
51.
52.
53.
54.
55.
Mary C. Orland 《Ecology letters》2003,6(8):716-720
Interactions between intrinsic processes and extrinsic fluctuations can positively impact population persistence in ways often not predicted by classic ecological models. These interactions only arise when the intrinsic and extrinsic processes operate on the proper relative scales in time or space. Both metapopulation theory and resonance/attenuation theory suggest that interactions which lower population variability will occur when the intrinsic and extrinsic process occur on similar time scales. I performed an aquatic protist microcosm experiment to investigate how the relative frequencies of extrinsic density perturbations and intrinsic resource pulses impacted population variability. Population variability was lowest in the treatments of intermediate frequency, in which the extrinsic fluctuations and intrinsic processes were on the same time scale. This result is consistent with general theoretical predictions, and empirically documents the importance of considering scale in interactions between intrinsic and extrinsic processes that positively impact population persistence. 相似文献
56.
57.
Holthuisana transversa reabsorbs much of its exoskeletal calcium in the last 3 days before ecdysis and stores it in circulating granules in the haemocoel and in non-circulating granules in the subepidermal connective tissue. Calcium enters the epidermal cells from the moulting fluid, probably through their apical microvilli and is either incorporated into intracellular calcium granules or exits the cell via the basolateral membranes to be used in formation of two other granule types. Intracellular granules (0.4–2 μm long) form in large masses in the apical cytoplasm of the epidermal cells. They are formed as membrane-bound vesicles by the Golgi, and calcium and organic matrix material are added from the surrounding cytoplasm. As development proceeds, lamellae appear and calcium carbonate is deposited in the matrix. Granule masses move basally and are stored in the connective tissue. Calcium is also incorporated into extracellular large granules (0.8–3.8 μm long) which are formed in narrow intercellular channels between epidermal cells. A third granule type (small granules, 0.26 μm diameter) is formed in subepidermal connective tissue cells and released into the haemolymph in very large numbers. Calcium was identified in the two larger granule types using X-ray microanalysis and significant amounts of phosphorus and potassium were also present in the large granules. A model for ion cycling between the exoskeleton and granules is presented. 相似文献
58.
59.
Calcium-Dependent Release of Tyrosine in Brain Elicited by Stimulation of Neuropeptide Receptors 总被引:1,自引:1,他引:0
We sought to establish whether the endogenous opiate-receptor agonist Met-enkephalin (m-ENK) selectively modulates the release of endogenous tyrosine (Tyr) from brain slices prepared from the corpus striatum (CS). Amino acids (AAs) released from slices of CS and, for comparison, cerebral cortex (Cx) were measured by HPLC. Incubation of slices with m-ENK (1-10 microM) increased the basal release of Tyr (up to 293% of control) from CS, but not Cx, whereas other nonneurotransmitter AAs, phenylalanine (Phe) and valine (Val), were unchanged. The release of the putative neurotransmitter AAs glutamate (Glu), taurine (Tau), and glycine (Gly) were similarly increased by 50-150% with m-ENK in slices of CS, but not Cx. The enhanced release of AAs by m-ENK was prevented by removal of extracellular Ca2+ or by preincubation with the opiate receptor antagonist naloxone. Neuronal depolarization by potassium (5-55 mM) in the presence of Ca2+ did not affect the release of Tyr, whereas release of neurotransmitter AAs such as gamma-aminobutyric acid (GABA) were markedly increased. The increase in basal Tyr release by m-ENK was not the result of a decreased uptake of Tyr. Relative to slices, the basal release of Tyr, Phe, and Val from a synaptosomal (P2) preparation of CS was small (8-51%) compared to that of GABA, Gly, Glu, and Tau (49-123%). Nonetheless, m-ENK (10 microM) markedly increased the release of Tyr (to 833%), but not Glu, Gly, and Tau from the P2 fraction.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
60.
Structure and expression of the overlapping ND4L and ND5 genes of Neurospora crassa mitochondria 总被引:1,自引:0,他引:1
Summary Genes homologous to the mammalian mitochondrial NADH dehydrogenase subunit genes ND4L and ND5 were identified in the mitochondrial genome of the filamentous fungus Neurospora crassa, and the structure and expression of these genes was examined. The ND4L gene (interrupted by one intervening sequence) potentially encodes an 89 residue long hydrophobic protein that shares about 26% homology (or 41% homology if conservative amino acid substitutions are allowed) with the analogous human mitochondrial protein. The ND5 gene (which contains two introns) encodes a 715 residue polypeptide that shares 23% homology with the human analogue; a 300 amino acid long region is highly conserved (50% homology) in the two ND5 proteins. The stop codon of the ND4L gene overlaps the initiation codon of the downstream ND5 gene, and the two genes are contranscribed and probably cotranslated. A presumed mature dicistronic (ND4L plus ND5) RNA was detected. The postulated mRNA (about 3.2 kb) contains 5 and 3 non-coding regions of about 86 and 730 nucleotides, respectively; this species is generated from very large precursor RNAs by a complex processing pathway. The ND4L and ND5 introns are all stable after their excision from the precursor species.Abbreviations bp
base pairs
- rRNA
ribosomal RNA
- ND
NADH dehydrogenase
- URF
unidentified reading frame
- kDal
kilodaltons; a.a., amino acid 相似文献