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91.
Naturally occurring benzodiazepines in human milk 总被引:1,自引:0,他引:1
C Pe?a J H Medina M Piva L E Diaz C Danilowicz A C Paladini 《Biochemical and biophysical research communications》1991,175(3):1042-1050
The presence of benzodiazepine-like molecules was detected radioimmunologically in the plasma and milk of 12 women and in the plasma of 9 men. All subjects were non-users of benzodiazepines. The concentration of these biological materials expressed as diazepam equivalents per mL amounted to 2.54 +/- 0.74 ng in male plasma; to 2.20 +/- 0.35 ng in female plasma and to 1.91 +/- 0.54 ng in milk. Further investigation of the active compounds in milk permitted the unequivocal identification of diazepam, both free and bound to a presumably protein carrier and, at least, three more benzodiazepine-like molecules. Their origin either from dietary sources or as a result of endogenous biosynthesis is still unclear. 相似文献
92.
Thyroid hormones control lipid composition and membrane fluidity of skeletal muscle sarcolemma. 总被引:1,自引:0,他引:1
Sarcolemma membrane lipid phase of skeletal muscles of hyperthyroid animals was compared to that of control (euthyroid) ones. Hyperthyroidism caused 15% decrease in cholesterol and 70% increase in the phospholipid content of the membrane. This was accompanied by the alterations in proportions between individual phospholipid classes, and was followed by changes in the composition of phospholipid fatty acids. The calculated fatty acid unsaturation index was higher for membrane lipid phase of hyperthyroid animals than of euthyroid ones. Thyroxine-induced alterations in the lipid composition of sarcolemma caused changes in the membrane fluidity and the activity of calmodulin-stimulated (Ca(2+)-Mg(2+)-ATPase. Measurements of the steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene indicated that the lipid phase transition of membrane vesicles occurred at 25.9 degrees C and at 28.9 degrees C for preparations isolated from hyperthyroid and euthyroid rabbits, respectively. Arrhenius plot break-point temperature for CaM-stimulated (Ca(2+)-Mg(2+)-ATPase activity was lower in membrane preparations isolated from hyperthyroid (26.9 degrees C) than from euthyroid ones (30.0 degrees C). Thus, the increase of the membrane fluidity presumably caused that the enzyme was characterized by the lower activation energy value. This phenomenon may be viewed as a supplementary mechanism for activation of the enzyme by thyroid hormones to previously reported elevation of the amount of (Ca(2+)-Mg(2+)-ATPase protein exerted by hyperthyroidism (Famulski et al. (1988) Eur. J. Biochem., 171, 363-368; Famulski and Wrzosek (1988) in The Ion Pumps-Structure, Function and Regulation (Stein, W.D., ed.), pp. 355-360, Alan R. Liss, New York). 相似文献
93.
J. Jesús García R. Snchez Olea H. Pasantes-Morales 《Journal of cellular biochemistry》1991,45(2):207-212
Rabbit lymphocytes exposed to hyposmotic media first swell and then recover their initial volume within 6 min. During volume recovery, free amino acids (FAA) decrease from 451.1 to 208 nmoles/mg protein. Taurine was the dominating FAA, accounting for 70% of the FAA pool. The time course of 3H-taurine release induced by hyposmolarity followed that of volume recovery. Efflux of 3H-taurine in an 8 min period was 17.8% (of total labeled taurine accumulated during loading) in an isosmotic medium. Reducing osmolarity to 0.87, 0.75, 0.62, and 0.5 increased this release to 24.8%, 38.1%, 56.4% and 70.9%, respectively. The volume-sensitive release of 3H-taurine was unaffected by omission of external Na+ or Ca++ and was reduced by 23% in the absence of Cl-. It was unaffected by agents disrupting the cytoskeleton or by tetraethylammonium, barium, quinidine, and gadolinium, but was 26% reduced by DIDS. Taurine release was inhibited at 4 degrees C, but was unchanged at 15 degrees C or 25 degrees C. An involvement of FAA, particularly taurine, in lymphocyte volume regulation is suggested. 相似文献
94.
In vivo and in vitro effects of temperature on monoamine oxidase activity in brain and other tissues of the goldfish. Carassius auratus L 总被引:1,自引:0,他引:1
T R Hall G Urue?a H R Figueroa 《Comparative biochemistry and physiology. C: Comparative pharmacology》1982,73(1):177-180
1. The maximum velocity (Vmax) and apparent Michaelis constant (Km) of brain and liver monoamine oxidase (MAO) in goldfish were different in fish acclimated to 22 degrees C and to 7 degrees C ambient temperature. 2. In brain, Vmax and Km were dependent upon incubation temperature, but both parameters were lower in 7 degrees C, adapted fish over most of the incubation temperature range. 3. The values obtained for Km showed a plateau at incubation temperatures at and below 25 degrees C for warm water fish, and at and below 20 degrees C for cold water fish. The activation energy of brain MAO was lower in fish adapted to the colder water. 4. These results show that goldfish MAO displays changes in functional activity in response to a change in environmental temperature. Apparently the purpose of this adaptation is to compensate for a reduction in enzyme concentration. 相似文献
95.
Carol J. Maslansky Gary M. Williams 《In vitro cellular & developmental biology. Plant》1982,18(8):683-693
Summary Hepatocyte primary cultures (HPC) derived from rat, mouse, hamster, and rabbit liver were characterized for a variety of parameters.
The conditions that maximized recovery, attachment, and survival varied between species. Hepatocytes from all four species
were capable of attaching in serum-free Williams’ medium E (WME), but optimal attachment as monolayer cultures was achieved
for mouse and hamster HPC in medium receiving 1% calf serum supplementation. Hamster hepatocytes required additional cations,
whereas rabbit and rat hepatocytes displayed maximal attachment in medium supplemented with 10% calf serum. Survival of mouse
and rabbit hepatocytes after 24 h in serum supplemented media was in the order of 90%. Rat and hamster hepatocyte 24 h survival
was approximately 70 and 60%, respectively, and was not significantly affected by serum supplementation. Hepatocytes from
each species varied in their content of cytochromeP450 at the time of isolation and in the rate of reduction during culture. Mouse and rat hepatocytes demonstrated the most
rapid decline in content during the initial 24 h in culture, whereas concentrations in rabbit hepatocytes were virtually unchanged.
The rate of decline inP450 concentrations in hamster hepatocytes was intermediate between those displayed by rat and rabbit hepatocytes. These studies
have delineated conditions useful for the culture of hepatocytes from four species and have documented the status of an important
parameter of their functional capability.
This study was supported by EPA contract 68-01-6179. C. J. Maslansky was a recipient of a Monsanto Fund Fellowship in Toxicology. 相似文献
96.
97.
98.
99.
Summary A mutant of S. fradiae producing higher amounts of tylosin than its parent also showed higher intracellular cAMP and DNA. Similarly the addition of chloroquine to producing cultures of the parent strain significantly increased the production of tylosin, cAMP, and DNA. The most likely hypothesis is that cAMP acts on tylosin production through a stimulation of the synthesis of DNA, which may prevent aging of the producing cells and lead to higher overvall antibiotic production. 相似文献
100.
A simple filter method for the fluorometric estimation of DNA in alkaline and neutral sucrose gradient fractions with DABA·2HCl is discussed. Alpha-450 membrane filters of regenerated cellulose (Gelman Instrument Co., Ann Arbor, Michigan) were used. In the proposed method washing of the DNA precipitate as well as the reaction with DABA·2HCl were performed directly on the filters, thus avoiding repeated washing and centrifugations of DNA precipitates applied hitherto in analogous fluorometric techniques. A good coincidence of the results concerning localization of DNA sedimentation profiles determined by radioisotopic and fluorometric methods was obtained. The method is very convenient for DNA estimation in alkaline and neutral sucrose density gradient fractions obtained by ultracentrifugation of DNA of nonproliferating cells where DNA labeling is very difficult, and in the case of human lymphocytes even impossible without stimulation for blastic transformation. Its other advantages are a considerably simplified procedure and a higher precision with respect to other fluorometric methods for determination of DNA. 相似文献