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61.
MG Oliveira Alves CFL Carta M-E Padín-Iruegas M Pérez-Sayáns JM Suarez-Peñaranda JS Issa 《Biotechnic & histochemistry》2016,91(4):263-268
We investigated the gene and protein expressions of V-type ATPase protein subunit C1 (ATP6V1C1) in cases of oral squamous cell carcinoma (OSCC) and contralateral normal mucosa in smokers, nonsmokers and former smokers. Subjects were separated into five groups of 15: group 1, smokers with OSCC; group 2, normal contralateral mucosa of OSCC patients; group 3, chronic smokers; group 4, former smokers who had stopped smoking 1 year earlier; group 5, individuals who had never smoked. Exfoliative cytology specimens from oral mucosa of smokers, former smokers and nonsmokers showed normal gene and protein expression. We found significantly greater gene expression in the OSCC group than in the nonsmoker groups. No difference in gene expression was observed between normal contralateral mucosa and nonsmoker groups, smoker and nonsmoker groups or former smoker and nonsmoker groups. We observed intense immunostaining for ATP6V1C1 protein in all cases of OSCC and weak or no staining in smoker, former smoker and nonsmoker groups. Significantly greater expression of ATP6V1C1 protein was observed in the OSCC group compared to the other groups, which supports the role of ATP6V1C1 in effecting changes associated with oral cancer. Analysis of the mucosae of chronic smokers, former smokers and the normal contralateral mucosa of patients with OSCC showed unaltered ATP6V1C1 gene and protein expression. Early stages of carcinogenesis, represented by altered epithelium of chronic smokers, had neither gene nor protein alterations as seen in OSCC. Therefore, we infer that the changes in ATP6V1C1 occur during later stages of carcinogenesis. Our preliminary study provides a basis for future studies of using ATP6V1C1 levels for detecting early stage OSCC. 相似文献
62.
Recovery of a recombinant cutinase with reversed micelles in a continuous perforated rotating disc contactor 总被引:1,自引:0,他引:1
M. G. Carneiro-da-Cunha M. R. Aires-Barros E. B. Tambourgi J. M. S. Cabral 《Biotechnology Techniques》1994,8(6):413-418
Summary A continuous perforated rotating disc contactor was used for the extraction of a recombinant cutinase from an aqueous solution to a reversed micellar phase of AOT in isooctane. Cutinase was extracted to the organic phase with protein yield of 78% after 70 minutes of operation. 相似文献
63.
M. G. Carneiro-da-Cunha M. R. Aires-Barros J. M. S. Cabral E. B. Tambourgi 《Bioprocess and biosystems engineering》1996,15(5):253-256
This work reports on the continuous extraction of a recombinant cutinase with reversed micelles using a perforated rotating
disc contactor. Intracellular cutinase was directly extracted at 20°C with 100 mM AOT in isooctane from complex biological
media of Escherichia coli disrupted cells. The optimal conditions for the direct extraction of the enzyme from media containing
cell debris led to an extraction yield of 54.4%. 相似文献
64.
5-Aminolevulinic acid (ALA), a precursor of porphyrin synthesis, increased the production of various porphyrin compounds in Candida guilliermondii cells. Metalloporphyrins and protoporphyrin IX (PPIX) were predominantly accumulated, respectively, at ALA concentrations of 0.2-0.4 mM and at those higher than 1.5 mM. 2,2;-Dipyridyl which complexed with bivalent metals significantly increased the content of endogenous PPIX even at ALA concentrations lower than 0.5 mM. Under these conditions, the yeast sensitivity to photodynamic effect of visible light (400-600 nm) dramatically increased due to photosensitization by endogenous PPIX. 相似文献