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991.
Carmen Rojas-Martínez Roger Iván Rodríguez-Vivas Julio Vicente Figueroa Millán Carlos Ramón Bautista-Garfias Roberto Omar Castañeda-Arriola José Juan Lira-Amaya Patricia Vargas Urióstegui Juan José Ojeda Carrasco Jesús Antonio Álvarez Martínez 《Parasitology international》2018,67(2):190-195
An attenuated live vaccine containing Babesia bovis and B. bigemina cultured in vitro with a serum-free medium was assessed for its clinical protection conferred of naïve cattle, under natural tick-challenge in a high endemicity zone to Babesia spp. Three groups of six animals were treated as follows: group I (GI) received a vaccine derived from parasites cultured with a free-serum medium; group II (GII) were immunized with the standard vaccine, with parasites cultured in a medium supplemented with 40% (v/v) bovine serum; and a control group (GIII) inoculated with non-infected bovine erythrocytes. Inocula were administered by IM route. Experimental animals were kept during 23 days after vaccination in a cattle farm free of ticks and Babesia spp. Thereafter, cattle were moved to a high endemicity farm for natural exposure to Babesia spp. transmitted by Rhipicephalus microplus ticks. Protection against clinical babesiosis was observed in bovines belonging to GI (100%) and GII (83.33%), while the control animals (GIII) were not protected, and showed severe clinical signs, closely related to babesiosis, were observed for at least three consecutive days during the challenge. These were fever, anemia, which were measured simultaneously, and circulating parasites were detected by optic light microscopy. All cattle showed B. bovis and B. bigemina in stained blood films during the challenge; B. bovis antibody titers were higher than those to B. bigemina in GI and GII, and lower titers were determined in GIII. The protective capacity of the vaccine derived from B. bovis and B. bigemina cultured in vitro in a serum-free medium was demonstrated. 相似文献
992.
Theresa Ohlig Do Viet Le Andreas Gardemann Carmen Wolke Sarah Gürtler Daniela Peter Lorenz Schild Uwe Lendeckel 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2018,1863(4):379-387
The mitochondrial phospholipid cardiolipin (CL) has been implicated with mitochondrial morphology, function, and cell proliferation. Changes in CL are often paralleled by changes in the lipid environment of mitochondria that may contribute to mitochondrial function and proliferation. This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72?h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of tafazzin had no consequence with respect to the pattern of cellular fatty acids but caused a decrease in cell proliferation. It significantly changed the distribution of molecular CL species, increased CL content, decreased oxygen consumption, and decreased cell proliferation when cultured in the presence of linoleic acid (LA). The addition of linoleic acid to the culture medium caused significant changes in the pattern of cellular fatty acids and the composition of molecular CL species. These data suggest that tafazzin is required for efficient bioenergetics and for proliferation of glioma cells. Supplementation of fatty acids can be a powerful tool to direct specific changes in these parameters. 相似文献
993.
Christopher?M.?SchalkEmail author Carmen?G.?Monta?a Kelsey?Kralman Daniel?J.?Leavitt 《Biological invasions》2018,20(8):1945-1952
More than 80% of animals have complex life cycles and undergo distinct changes in ecology and morphology during development. The strength and type of factors regulating each life-stage may differ as an organism may occupy different niches during ontogeny. We examined the functional distance at larval and adult life-stages of two non-native anurans (Green Tree Frog [Hyla cinerea] and Bullfrog [Lithobates catesbeianus]) that have established in a Chihuahuan Desert anuran assemblage in Big Bend National Park. Both life stages of both non-native species occupied niche space outside of the native assemblage. At the larval stage, the ability of the tadpoles to utilize permanent aquatic habitats and coexist with predatory fishes differentiated the non-native species from the majority of the native species that are restricted to temporary pools. At the post-metamorphic life stage, each species appears to have established by exploiting unoccupied habitat and trophic niches in the recipient community. The arboreal habits of H. cinerea may enable it to utilize resources in microhabitats that are otherwise not used by native species because arboreal frogs are absent from this native assemblage. The large body size of post-metamorphic L. catesbeianus may enable it to utilize larger food resources that are otherwise unavailable to the smaller-bodied natives. Separate comparison of larval and adult functional traits between non-natives and the native community may help predict their potential establishment or invasion success as well as aid in the development of stage-specific control or eradication efforts. 相似文献
994.
Adult Sox2+ stem cell exhaustion in mice results in cellular senescence and premature aging
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Jéssica M. Vilas Carmen Carneiro Sabela Da Silva‐Álvarez Alba Ferreirós Patricia González María Gómez Sagrario Ortega Manuel Serrano Tomás García‐Caballero Miguel González‐Barcia Anxo Vidal Manuel Collado 《Aging cell》2018,17(5)
Aging is characterized by a gradual functional decline of tissues with age. Adult stem and progenitor cells are responsible for tissue maintenance, repair, and regeneration, but during aging, this population of cells is decreased or its activity is reduced, compromising tissue integrity and causing pathologies that increase vulnerability, and ultimately lead to death. The causes of stem cell exhaustion during aging are not clear, and whether a reduction in stem cell function is a cause or a consequence of aging remains unresolved. Here, we took advantage of a mouse model of induced adult Sox2+ stem cell depletion to address whether accelerated stem cell depletion can promote premature aging. After a short period of partial repetitive depletion of this adult stem cell population in mice, we observed increased kyphosis and hair graying, and reduced fat mass, all of them signs of premature aging. It is interesting that cellular senescence was identified in kidney after this partial repetitive Sox2+ cell depletion. To confirm these observations, we performed a prolonged protocol of partial repetitive depletion of Sox2+ cells, forcing regeneration from the remaining Sox2+ cells, thereby causing their exhaustion. Senescence specific staining and the analysis of the expression of genetic markers clearly corroborated that adult stem cell exhaustion can lead to cellular senescence induction and premature aging. 相似文献
995.
Wood mice aggressiveness and flight response to human handling: Effect of individual and environmental factors
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MªCarmen Hernández Álvaro Navarro‐Castilla Ana Piñeiro Isabel Barja 《Ethology : formerly Zeitschrift fur Tierpsychologie》2018,124(8):559-569
Numerous studies have examined human disturbance repercussions on wildlife, mainly focused on the effects on behaviour, reproductive success and population dynamics. However, few studies have addressed the behaviour of prey species during and after human capture and handling and how this may correlate to individual characteristics or variation in their physical environment they inhabit. We explored wood mouse’s fleeing and aggressive behaviours in response to captures by human in their natural habitat. Eighty‐seven wood mice were caught using Sherman live traps. For each trapped individual, aggressiveness was measured as the total number of bites inflicted upon the investigator during handling time. Afterwards, each mouse was released in a two‐metre radius partially covered vegetation area that allowed visual mouse tracking by the observer and flight behaviour was registered by individual one‐zero focal sampling technique during 2 min. Both aggressiveness and fleeing behaviour were analysed regarding individual (sex, reproductive status, age) and environmental factors (habitat and season). Males, adults and breeding individuals showed heightened aggression levels. Higher aggressiveness levels were found in wood mice occupying scrubland and during summer and autumn. The flight response was exclusively explained by reproductive status, whereby breeding individuals spent more time on fast escape than nonbreeding ones. These results indicate that both individual and environmental factors seem to influence defensive behaviours in the wood mouse during and after being captured by a human. Since human disturbance shares many aspects with the predation risk, behavioural responses found to captures may likely be influenced by previous experience of individuals with predators as well as to seasonal and habitat features conditioning predators’ densities but also protection against them. 相似文献
996.
Krolicka Malgorzata Hinz Sandra W. A. Koetsier Martijn J. Eggink Gerrit van den Broek Lambertus A. M. Boeriu Carmen G. 《Applied microbiology and biotechnology》2018,102(17):7441-7454
Applied Microbiology and Biotechnology - Thermostable enzymes are a promising alternative for chemical catalysts currently used for the production of N-acetylglucosamine (GlcNAc) from chitin. In... 相似文献
997.
998.
Wenxue Li Aurelie Papilloud Laura Lozano‐Montes Nan Zhao Xueting Ye Xiaozhe Zhang Carmen Sandi Gregor Rainer 《Proteomics》2018,18(7)
Adverse life experiences increase the lifetime risk to several stress‐related psychopathologies, such as anxiety or depressive‐like symptoms following stress in adulthood. However, the neurochemical modulations triggered by stress have not been fully characterized. Neuropeptides play an important role as signaling molecules that contribute to physiological regulation and have been linked to neurological and psychiatric diseases. However, little is known about the influence of stress on neuropeptide regulation in the brain. Here, we have performed an exploratory study of how neuropeptide expression at adulthood is modulated by experiencing a period of multiple stressful experiences. We have targeted hippocampus and prefrontal cortex (PFC) brain areas, which have previously been shown to be modulated by stressors, employing a targeted liquid chromatography‐mass spectrometry (LC‐MS) based approach that permits broad peptide coverage with high sensitivity. We found that in the hippocampus, Met‐enkephalin, Met‐enkephalin‐Arg‐Phe, and Met‐enkephalin‐Arg‐Gly‐Leu were upregulated, while Leu‐enkephalin and Little SAAS were downregulated after stress. In the PFC area, Met‐enkephalin‐Arg‐Phe, Met‐enkephalin‐Arg‐Gly‐Leu, peptide PHI‐27, somatostatin‐28 (AA1‐12), and Little SAAS were all downregulated. This systematic evaluation of neuropeptide alterations in the hippocampus and PFC suggests that stressors impact neuropeptides and that neuropeptide regulation is brain‐area specific. These findings suggest several potential peptide candidates, which warrant further investigations in terms of correlation with depression‐associated behaviors. 相似文献
999.
Rita Cruz Fernando Esteves Carmen Vasconcelos-Nóbrega Carla Santos Ana Sofia Ferreira Ana Cristina Mega Ana Claudia Coelho Helena Vala João Rodrigo Mesquita 《EcoHealth》2018,15(4):871-874
Q fever is a zoonotic disease caused by Coxiella burnetii that is highly prevalent across the world. In this study, a prospective serosurvey was performed to study C. burnetii circulation in a population of sheep in the central region of Portugal. Blood from a representative sample of 168 animals was drawn in both 2015 and 2016, and sera were tested for IgG anti-C. burnetii by EIA. In 2015, 7.7% (13/168) animals tested positive for IgG anti-C. burnetii, while in 2016, 17.3% (29/168) tested positive, showing a statistically significant (P?=?0.008) increase in anti-C. burnetii seroprevalence. Results support the notion that Q fever is emerging in central Portugal. 相似文献