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Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium. 总被引:4,自引:2,他引:2 下载免费PDF全文
A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic maps of megacinogenic plasmids pBM309 and pBM113. Limited sequence homology between pBM309 and pBM113 was detected by Southern blot hybridization and was mapped to, at most, a 6.4-kilobase-pair region of pBM309 and a 6.1-kilobase-pair region of pBM113. 相似文献
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Temporary sterility produced in male mice by 5-thio-D-glucose. 总被引:1,自引:0,他引:1
J R Zysk A A Bushway R L Whistler W W Carlton 《Journal of reproduction and fertility》1975,45(1):69-72
When 5-thio-D-glucose was fed to male mice at daily dose levels greater than 30 mg/kg sperm development was completely inhibited within 3 weeks and remained so without impairment of libido for the experimental period of 7 weeks. Removal of this substance from the diet resulted in a resumption of sperm development and fertility within 5 to 8 weeks. Normal litters were sired by males which had recovered after this treatment. 相似文献
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Barnett Carlton C. Jr.; Moore Ernest E.; Mierau Gary W.; Partrick David A.; Biffl Walter L.; Elzi David J.; Silliman Christopher C. 《American journal of physiology. Cell physiology》1998,274(6):C1634
Interaction ofthe 2-integrin complex on thepolymorphonuclear neutrophil (PMN) with intercellular adhesionmolecule-1 (ICAM-1) has been implicated in PMN-mediated cytotoxicity.This study examined interaction of the CD11a, CD11b, and CD18 subunitsof the 2-integrin with ICAM-1,transfected into Chinese hamster ovarian (CHO) cells to avoid effectsof other adhesion molecules. Incubation of quiescent PMNs withwild-type and ICAM-1-transfected CHO cells produced nominal cell lysis.Similarly, when phorbol myristate acetate (PMA)-activated PMNs wereincubated with wild-type CHO cells, minimal cytotoxicity was produced.However, when ICAM-1-transfected CHO cells were incubated withPMA-activated PMNs, 40% cell lysis occurred. Blockade with amonoclonal antibody (MAb) to ICAM-1 or MAbs to CD11a, CD11b, or CD18reduced PMN-mediated cytotoxicity to baseline. To examine the role ofadhesion in cytotoxicity, we studied2-integrin-mediated PMNadhesion to ICAM-1-transfected CHO cells and found that MAbs for CD11a,CD11b, and CD18 all abrogated PMN cytotoxicity despite disparateeffects on adhesion. To assess the role of CD18,2-integrin subunits werecross-linked, and CD18 alone mediated protease release. Moreover,ICAM-1 was immunoprecipitated from transfected CHO cells and incubatedwith PMNs. This soluble ICAM-1 provoked elastase release, similar toPMA, which could be inhibited by MAbs to CD18 but not MAbs to other2-integrin subunits. Inaddition, coincubation with protease inhibitors eglin C and AAPVCKreduced PMN-mediated cytotoxicity to control levels. Finally,ICAM-1-transfected CHO cells were exposed to activated PMNs from apatient with chronic granulomatous disease that caused significant celllysis, equivalent to that of PMNs from normal donors. Collectively,these data suggest that ICAM-1 provokes PMN-mediated cytotoxicity viaCD18-mediated protease release. 相似文献
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